Chikara Aizawa

C1q solid-phase radioimmunoassay: Binding properties of solid-phase C1q and evidence that C1q-binding IgG complexes in systemic lupus erythematosus are not bound to endogenous C1q

The binding properties of C1q solid-phase radioimmunoassay (C1q SPRIA) were examined, using heat-aggregated IgG (HAG) as the model of immune complexes (IC). The free, liquid-phase C1q, which was added to the C1q-coated tubes prior to the addition of HAG, had little inhibitory effect on binding of HAG to the solid-phase C1q, suggesting that the solid-phase C1q has a higher affinity for HAG than the liquid-phase C1q. On the other hand, more than 60% inhibition was seen when HAG was preincubated with the liquid-phase C1q. These binding properties of HAG to the solid-phase C1q in the presence of the liquid-phase C1q were not essentially altered by the heat inactivation or the addition of EDTA, suggesting that these pretreatments are not essential in C1q SPRIA. Next, in similar kinds of experiments, the binding properties of C1q-binding IgG complexes in SLE sera were investigated. In contrast to HAG, the binding capacity of IgG complexes in SLE sera to the solid-phase C1q was not inhibited by the preincubation with excess liquid-phase C1q. These findings suggest that C1q-binding IgG complexes in SLE sera detected by C1q SPRIA may not be bound to endogenous C1q in the circulation.

A CASE OF SARCOIDOSIS ASSOCIATED WITH OCCLUSION OF AQUEDUCT OF MIDBRAIN AND INAPPROPRIATE SECRETION OF ADH SYNDROME

サルコイドーシスの経過中,中脳水道閉塞によると思われる頭痛,悪心,嘔吐,めまい.難聴,記銘力・指南力低下などの症状を呈し, 副腎皮質ステロイド薬投与および髄液圧減圧術により,一時,小康をえたが,再び,記銘力・指南力低下とともに,低Na,低Cl血症,尿中Na, Cl排泄増加,高張尿などの所見を呈し,腎機能に異常なく,副腎機能不全もないことから, ADH異常分泌症候群と考えられた症例を報告する.本例の脳圧亢進症状,水電解質代謝異常は副腎皮質ステロイド薬が著効を示したことから,いずれもサルコイドーシスによると考えた.