Choemon Kanno

Antigenic Identity between the Soluble Glycoprotein of Milk Fat Globule Membrane and a Heat-stable Protein Fraction of Whey

The protein which is antigenically equivalent to the soluble glycoprotein (SGP) isolated from milk fat globule membrane was immunologically found to be contained in whey protein. The identical antigeneicity between the SGP and an unknown protein component of whey was certified by double immunodiffusion and immunoelectrophoretic assays, using the antiserum of the SGP and its antiserum absorbed with whey protein, and using the whey protein fractions fractionated by gel filtration or ion exchange chromatography. The concentration of this protein in whey (anti-SGP reacting protein) was estimated to be about 15% of total whey protein by simple radial immunodiffusion assay. The major whey protein components, such as ƒÀ-lactoglobulin, ƒ¿-lactalbumin, bovine serum albumin, and IgG-immunoglobulin were confirm-d to be not the anti-SGP reacting