Chris G. Whiteley

Bacterial diguanylate cyclases: Structure, function and mechanism in exopolysaccharide biofilm development

The ubiquitous bacterial cyclic di-guanosine monophosphate (c-di-GMP) emerges as an important messenger for the control of many bacterial cellular functions including virulence, motility, bioluminescence, cellulose biosynthesis, adhesion, secretion, community behaviour, biofilm formation and cell differentiation. The synthesis of this cyclic nucleotide arises from external stimuli on various signalling domains within the N-terminal region of a dimeric diguanylate cyclase. This initiates the condensation of two molecules of guanosine triphosphate juxtaposed to each other within the C-terminal region of the enzyme. The biofilm from pathogenic microbes is highly resistant to antimicrobial agents suggesting that diguanylate cyclase and its product - c-di-GMP - are key biomedical targets for the inhibition of biofilm development. Furthermore the formation and long-term stability of the aerobic granule, a superior biofilm for biological wastewater treatment, can be controlled by stimulation of c-di-GMP. Any modulation of the synthetic pathways for c-di-GMP is clearly advantageous in terms of medical, industrial and/or environmental bioremediation implications. This review discusses the structure and reaction of individual diguanylate cyclase enzymes with a focus on new directions in c-di-GMP research. Specific attention is made on the molecular mechanisms that control bacterial exopolysaccharide biofilm formation and aerobic granules.

A passive auxiliary circuit with interphase transformer applied in 12-pulse converters to provide clean power utility interface

Abstract This paper proposes a passive auxiliary circuit which can be added to an interphase transformer (PAC + IPT) configuration to reduce the total harmonic distortion (THD) existing in 12-pulse diode rectifier converter systems at AC mains. The proposed PAC + IPT compensation method is a simple structure, with low power consumption and requires no extra DC power supply. We present the theoretical analysis of the proposed topology that lessens the total harmonic distortion (THD) and evaluate the dynamic simulation results on a 12-pulse converter system and a 3-kW laboratory prototype. Both the simulation and the experimental results show that the proposed PAC + IPT compensation method can improve the power quality and provide a clean power utility interface of AC line input currents for a conventional 12-pulse diode rectifier converter.