Chris O'Donnell

Single-site mutations in the carboxyltransferase domain of plastid acetyl-CoA carboxylase confer resistance to grass-specific herbicides

Grass weed populations resistant to aryloxyphenoxypropionate (APP) and cyclohexanedione herbicides that inhibit acetyl-CoA carboxylase (ACCase; EC 6.4.1.2) represent a major problem for sustainable agriculture. We investigated the molecular basis of resistance to ACCase-inhibiting herbicides for nine wild oat (Avena sterilis ssp. ludoviciana Durieu) populations from the northern grain-growing region of Australia. Five amino acid substitutions in plastid ACCase were correlated with herbicide resistance: Ile-1,781-Leu, Trp-1,999-Cys, Trp-2,027-Cys, Ile-2,041-Asn, and Asp-2,078-Gly (numbered according to the Alopecurus myosuroides plastid ACCase). An allele-specific PCR test was designed to determine the prevalence of these five mutations in wild oat populations suspected of harboring ACCase-related resistance with the result that, in most but not all cases, plant resistance was correlated with one (and only one) of the five mutations. We then showed, using a yeast gene-replacement system, that these single-site mutations also confer herbicide resistance to wheat plastid ACCase: Ile-1,781-Leu and Asp-2,078-Gly confer resistance to APPs and cyclohexanediones, Trp-2,027-Cys and Ile-2,041-Asn confer resistance to APPs, and Trp-1,999-Cys confers resistance only to fenoxaprop. These mutations are very likely to confer resistance to any grass weed species under selection imposed by the extensive agricultural use of the herbicides.

Spray deposition on plant surfaces: a modelling approach

For pesticides to effectively manage pests, they must first be deposited on the target (typically a plant surface) in a manner in which the active ingredient(s) can be readily taken up by the target organism. A plant architectural model that enables the location of various plant components in 3-D space combined with a particle trajectory model has been used to study the interception of spray droplets by various vegetative elements. Results from the simulation are compared with wind tunnel studies of glyphosate deposition on cotton (Gossypium hirsutum L. var. Sicala), sow thistle (Sonchus oleraceus L.) and wild oats (Avena ludoviciana Durieu). An air induction flat fan nozzle (AI110015 at 500u2009kPa pressure) and an extended range flat fan nozzle (XR11002 at 280u2009kPa pressure) were predicted to have similar glyphosate deposition on cotton and sow thistle plants, whereas the extended range nozzle resulted in higher deposit on wild oats. Spray deposition (µgu2009cm-2) on wild oat plants at the 5-leaf stage was more than double the amount of deposition on sow thistle or wild oat plants at the 2-leaf stage. The model was in good agreement with the experimental data except that it tended to over predict deposition on sow thistle plants.

Wild oat and climate change: The effect of CO2 concentration, temperature, and water deficit on the growth and development of wild oat in monoculture

Abstract Seed from six Australian near-isogenic lines of wild oat were germinated and grown in controlled-environment growth chambers under either ambient CO2 (357 parts per million by volume [ppmv]) or elevated CO2 (480 ppmv) at 20/16 C or 23/19 C. Three soil moisture treatments—−0.01 MPa (field capacity), −0.10 MPa, or −1.00 MPa—were imposed. Wild oat lines grown under elevated CO2 had higher seed production and greater plant dry weights, although the response of these variates involved a complex of interactions with temperature, soil moisture, and line. Plant height varied with wild oat line, and plants grown at 20/16 C were taller than those grown at 23/19 C. At 23/19 C, time taken to mature was reduced for some wild oat lines, and elevated CO2 reduced the time taken to maturity for some lines at 20/16 C. There was no significant difference in the level of dormancy developed in freshly harvested caryopses between the two CO2 treatments, but an effect was present in seed that had been after-ripened for 193 d. These results indicate that the main climate change variables ([CO2], soil moisture, and increased temperature) directly influence the growth and development of wild oat and are likely to affect the population dynamics of this species. Nomenclature: Wild oat, Avena fatua AVEFA.

Determining the drift potential of Venturi nozzles compared with standard nozzles across three insecticide spray solutions in a wind tunnel

BACKGROUNDnPrevious research has sought to adopt the use of drift-reducing technologies (DRTs) for use in field trials to control diamondback moth (DBM) Plutella xylostella (L.) (Lepidoptera: Plutellidae) in canola (Brassica napus L.). Previous studies observed no difference in canopy penetration from fine to coarse sprays, but the coverage was higher for fine sprays. DBM has a strong propensity to avoid sprayed plant material, putting further pressure on selecting technologies that maximise coverage, but often this is at the expense of a greater drift potential. This study aims to examine the addition of a DRT oil that is labelled for control of DBM as well and its effect on the drift potential of the spray solution. The objectives of the study are to quantify the droplet size spectrum and spray drift potential of each nozzle type to select technologies that reduce spray drift, to examine the effect of the insecticide tank mix at both (50 and 100 L ha(-1) ) application rates on droplet size and spray drift potential across tested nozzle type and to compare the droplet size results of each nozzle by tank mix against the drift potential of each nozzle.nnnRESULTSnThe nozzle type affected the drift potential the most, but the spray solution also affected drift potential. The fine spray quality (TCP) resulted in the greatest drift potential (7.2%), whereas the coarse spray quality (AIXR) resulted in the lowest (1.3%), across all spray solutions. The spray solutions mixed at the 100 L ha(-1) application volume rate resulted in a higher drift potential than the same products mixed at the 50 L ha(-1) mix rate. The addition of the paraffinic DRT oil was significant in reducing the drift potential of Bacillus thuringiensis var. kurstkai (Bt)-only treatments across all tested nozzle types. The reduction in drift potential from the fine spray quality to the coarse spray quality was up to 85%.nnnCONCLUSIONnThe addition of a DRT oil is an effective way to reduce the spray solution drift potential across all nozzle types and tank mixes evaluated in this study. The greatest reduction in drift potential can be achieved by changing nozzle type, which can reduce the losses of the spray to the surrounding environment. Venturi nozzles greatly reduce the drift potential compared with standard nozzles by as much as 85% across all three insecticide spray solutions. Results suggest that a significant reduction in drift potential can be achieved by changing the nozzle type, and can be achieved without a loss in control of DBM.

Australian university productivity growth and public funding revisited

ABSTRACT The Australian Government provides basic operating grants to universities, which are used to teach domestic undergraduate students. It imposes a productivity offset on the grants to encourage improvements in university productivity. But it is not transparent and does not vary across universities. Thus, universities have little incentive to improve performance. This paper develops an alternative framework that uses incentive regulation to allocate these grants to universities, which provides stronger incentives for universities to improve productivity. Regulators often use a similar framework to set prices for natural monopoly services such as utilities and public transport. Under incentive regulation, the basic operating grants could be reduced, on average, by 1.76% per annum over 5 years, which is about

Weed seed spread and its prevention: The role of roadside wash down

100 million per annum. This finding is contrary to several recent Australian Government inquires that suggest the basic operating grant is inadequate and that this compromises the quality of undergraduate teaching.

Assessing a novel smartphone application - SnapCard, compared to five imaging systems to quantify droplet deposition on artificial collectors

Vehicles are one of the major vectors of long-distance weed seed spread. Viable seed removed from vehicles at roadside wash down facilities was studied at five locations in central Queensland, Australia over a 3-year period. Seed from 145 plant species, belonging to 34 different families, were identified in the sludge samples obtained from the wet particulate matter collection pit of the wash down facilities. Most of the species were annual forbs (50%) with small or very small seed size (<2xa0mm in diameter). A significant amount of seed from the highly invasive, parthenium weed was observed in these samples. More parthenium weed seed were found in the Rolleston facility and in the spring, but its seed was present in all facilities and in all seasons. The average number of viable seed found within every ton of dry particulate matter removed from vehicles was ca. 68,000. Thus, a typical wash down facility was removing up to ca. 335,000 viable seed from vehicles per week, of which ca. 6700 were parthenium weed seed. Furthermore, 61% of these seed (ca. 200,000) were from introduced species, and about half of these (35% of total) were from species considered to be weeds. Therefore, the roadside wash down facilities found throughout Queensland can remove a substantial amount of viable weed seed from vehicles, including the invasive parthenium weed, and the use of such facilities should be strongly encouraged.

Determining the uniformity and consistency of droplet size across spray drift reducing nozzles in a wind tunnel

SnapCard measured coverage precisely compared with industry standard image analysis software.Kromekote is comparable to water-sensitive paper for coverage measurements.Spray quality is an important factor influencing coverage.SnapCard is a reliable tool for in-field image analysis of sprayed collectors. Previous work sought to compare the results from imaging software for characterising droplet coverage, but none exists examining these five software programs: Droplet Scanź, Swath Kitź, Deposit Scan, Image J, and Drop Visionź-Ag. Additionally, a freely available smartphone application (App), SnapCard was developed to provide an extension tool for in-field analysis of spray collectors, but nothing has been published regarding its comparison to other imaging software systems. The present study was conducted to compare five existing imaging software types against the new App, SnapCard. Six nozzles producing different spray qualities were selected to spray a water+Brilliant Blue Dye solution over two artificial collector types (water sensitive paper and Kromekoteź). Each collector was assessed for percent coverage using the five imaging systems and SnapCard. Objectives of this study were: 1. To establish a baseline dataset using the sprayed cards and five commonly used imaging systems, and compare the coverage results from each. 2. Use the baseline data from Objective 1 as a measurement of precision to judge the results from SnapCard. 3. Make an assessment of SnapCard against the other imaging software type data in the study.Results showed that SnapCard has similar measured coverage means compared to other image analysis systems. For both collector types, SnapCard measured coverage within one standard deviation of the means across nozzle types. SnapCard is able to provide an immediate answer without expensive software or needing a laboratory to measure sprayed collector coverage with precise results, which further underscores its value. The other software types were not all similar for coverage, but the data followed the same trends for droplet size. Increasing the droplet size consistently decreased the coverage, across both collector types. Droplet Scan reported the highest coverage while Drop Vision-Ag and Swath Kit gave lower coverage values on water sensitive paper and Kromekoteź collectors, respectively.