Christel Kampman

Bioelectrochemical Ethanol Production through Mediated Acetate Reduction by Mixed Cultures

Biological acetate reduction with hydrogen is a potential method to convert wet biomass waste into ethanol. Since the ethanol concentration and reaction rates are low, this research studies the feasibility of using an electrode, in stead of hydrogen, as an electron donor for biological acetate reduction in conjunction of an electron mediator. Initially, the effect of three selected mediators on metabolic flows during acetate reduction with hydrogen was explored; subsequently, the best performing mediator was used in a bioelectrochemical system to stimulate acetate reduction at the cathode with mixed cultures at an applied cathode potential of -550 mV. In the batch test, methyl viologen (MV) was found to accelerate ethanol production 6-fold and increased ethanol concentration 2-fold to 13.5 +/- 0.7 mM compared to the control. Additionally, MV inhibited n-butyrate and methane formation, resulting in high ethanol production efficiency (74.6 +/- 6%). In the bioelectrochemical system, MV addition to an inoculated cathode led directly to ethanol production (1.82 mM). Hydrogen was coproduced at the cathode (0.0035 Nm(3) hydrogen m(-2) d(-1)), so it remained unclear whether acetate was reduced to ethanol by electrons supplied by the mediator or by hydrogen. As MV reacted irreversibly at the cathode, ethanol production stopped after 5 days.

Autotrophic nitrogen removal from low strength waste water at low temperature.

Direct anaerobic treatment of municipal waste waters allows for energy recovery in the form of biogas. A further decrease in the energy requirement for waste water treatment can be achieved by removing the ammonium in the anaerobic effluent with an autotrophic process, such as anammox. Until now, anammox has mainly been used for treating warm (>30 °C) and concentrated (>500 mg N/L) waste streams. Application in the water line of municipal waste water treatment poses the challenges of a lower nitrogen concentration (<100 mg N/L) and a lower temperature (≤ 20 °C). Good biomass retention and a short HRT are required to achieve a sufficiently high nitrogen loading rate. For this purpose a 4.5 L gaslift reactor was inoculated with a small amount of anammox granules and operated for 253 days at 20 °C. The synthetic influent contained (69 ± 5) mg (NH(4)(+) + NO(2)(-))/L and 20 vol.% of anaerobically stabilised effluent. Results showed a clear increase in nitrogen loading rate (NLR) up to 0.31 g (NH(4) + NO(2))-N/(L × d) at a hydraulic retention time (HRT) of 5.3 h. A low effluent concentration of 0.03-0.17 mg (NH(4)(+)+NO(2)(-))-N/L could be achieved. Anammox biomass was retained as granules and as a biofilm on the reactor walls, which contributed 54 and 46%, respectively, towards total activity. The biomass was further characterised by an estimated net growth rate of 0.040 d(-1) and an apparent activation energy of 72 kJ/mol. The results presented in this paper showed that anammox bacteria can be applied for autotrophic nitrogen removal from the water line at a municipal waste water treatment plant. Combining direct anaerobic treatment with autotrophic nitrogen removal opens opportunities for energy-efficient treatment of municipal waste waters.

High specific activity for anammox bacteria enriched from activated sludge at 10 °C

Anammox in the water line of a waste water treatment plant (WWTP) saves energy for aeration and allows for recovering biogas from organic material. Main challenges for applying the anammox process in the water line are related to the low temperature of <20°C, causing a significant drop in the specific anammox activity. The aim of this research was to enrich a cold-adapted anammox species, with a high specific activity. This was achieved in a 4.2L reactor operated at 10°C, fed with 61 mg (NH4+NO2)-N/L and inoculated with activated sludge from two selected municipal WWTPs. Candidatus Brocadia fulgida was the dominant species in the enriched biomass, with a specific activity was 30-44 mg N/(g VS d). This is two times higher than previously reported at 10°C, which is beneficial for full scale application. Biomass yield was 0.046 g biomass/g N converted, similar to that at higher temperatures.

Co-digestion to support low temperature anaerobic pretreatment of municipal sewage in a UASB-digester.

The aim of this work was to demonstrate that co-digestion improves soluble sewage COD removal efficiency in treatment of low temperature municipal sewage by a UASB-digester system. A pilot scale UASB-digester system was applied to treat real municipal sewage, and glucose was chosen as a model co-substrate. Co-substrate was added in the sludge digester to produce additional methanogenic biomass, which was continuously recycled to inoculate the UASB reactor. Soluble sewage COD removal efficiency increased from 6 to 23%, which was similar to its biological methane potential (BMP). Specific methanogenic activity of the UASB and of the digester sludge at 15°C tripled to a value respectively of 43 and 39 mg CH4-COD/(g VSS d). Methane production in the UASB reactor increased by more than 90% due to its doubled methanogenic capacity. Therefore, co-digestion is a suitable approach to support a UASB-digester for pretreatment of low temperature municipal sewage.

The effect of sludge recirculation rate on a UASB-digester treating domestic sewage at 15 °C

The anaerobic treatment of low strength domestic sewage at low temperature is an attractive and important topic at present. The upflow anaerobic sludge bed (UASB)-digester system is one of the anaerobic systems to challenge low temperature and concentrations. The effect of sludge recirculation rate on a UASB-digester system treating domestic sewage at 15 °C was studied in this research. A sludge recirculation rate of 0.9, 2.6 and 12.5% of the influent flow rate was investigated. The results showed that the total chemical oxygen demand (COD) removal efficiency rose with increasing sludge recirculation rate. A sludge recirculation rate of 0.9% of the influent flow rate led to organic solids accumulation in the UASB reactor. After the sludge recirculation rate increased from 0.9 to 2.6%, the stability of the UASB sludge was substantially improved from 0.37 to 0.15 g CH₄-COD/g COD, and the bio-gas production in the digester went up from 2.9 to 7.4 L/d. The stability of the UASB sludge and bio-gas production in the digester were not significantly further improved by increasing sludge recirculation rate to 12.5% of the influent flow rate, but the biogas production in the UASB increased from 0.37 to 1.2 L/d. It is recommended to apply a maximum sludge recirculation rate of 2-2.5% of the influent flow rate in a UASB-digester system, as this still allows energy self-sufficiency of the system.