Christer Sundqvist

Identification of four universal protochlorophyllide forms in dark-grown leaves by analyses of the 77 K fluorescence emission spectra

Abstract Low-temperature (77 K) fluorescence emission spectra of dark-grown leaves from 12 different plant species were measured, corrected and analysed at different stages of development. The second and fourth derivative of the spectra indicated the peak positions of possible spectral bands, which were used for resolution of the fluorescence spectra into gaussian components. Based on the intensities of the gaussian components, statistical analyses were performed to determine a possible connection between the main peaks and their respective long-wavelength vibrational sublevels. Four universal protochloro-phyll(ide) forms were distinguished, with the main emission bands (half-bandwidths parentheses) at 633 (14.5), 645 (14.3), 657 (10.5) and 670 (20.1) nm respectively. Their vibrational sublevels were found at 693 (20.8), 710 (17.3), 726 (17.7) and 740 (21.0) nm respectively. The spectral characteristics of the different protochlorophyll(ide) forms are discussed in relation to their possible molecular structures and interactions with the surrounding membrane components.

Monogalactosyldiacylglycerol Deficiency in Arabidopsis Affects Pigment Composition in the Prolamellar Body and Impairs Thylakoid Membrane Energization and Photoprotection in Leaves

Monogalactosyldiacylglycerol (MGDG) is the major lipid constituent of chloroplast membranes and has been proposed to act directly in several important plastidic processes, particularly during photosynthesis. In this study, the effect of MGDG deficiency, as observed in the monogalactosyldiacylglycerol synthase1-1 (mgd1-1) mutant, on chloroplast protein targeting, phototransformation of pigments, and photosynthetic light reactions was analyzed. The targeting of plastid proteins into or across the envelope, or into the thylakoid membrane, was not different from wild-type in the mgd1 mutant, suggesting that the residual amount of MGDG in mgd1 was sufficient to maintain functional targeting mechanisms. In dark-grown plants, the ratio of bound protochlorophyllide (Pchlide, F656) to free Pchlide (F631) was increased in mgd1 compared to the wild type. Increased levels of the photoconvertible pigment-protein complex (F656), which is photoprotective and suppresses photooxidative damage caused by an excess of free Pchlide, may be an adaptive response to the mgd1 mutation. Leaves of mgd1 suffered from a massively impaired capacity for thermal dissipation of excess light due to an inefficient operation of the xanthophyll cycle; the mutant contained less zeaxanthin and more violaxanthin than wild type after 60 min of high-light exposure and suffered from increased photosystem II photoinhibition. This is attributable to an increased conductivity of the thylakoid membrane at high light intensities, so that the proton motive force is reduced and the thylakoid lumen is less acidic than in wild type. Thus, the pH-dependent activation of the violaxanthin de-epoxidase and of the PsbS protein is impaired.

Leaf Developmental Age Controls Expression of Genes Encoding Enzymes of Chlorophyll and Heme Biosynthesis in Pea (Pisum sativum L.)

The effects of leaf developmental age on the expression of three nuclear gene families in pea (Pisum sativum L.) coding for enzymes of chlorophyll and heme biosynthesis have been examined. The steady-state levels of mRNAs encoding aminolevulinic acid (ALA) dehydratase, porphobilinogen (PBG) deaminase, and NADPH:protochlorophyllide reductase were measured by RNA gel blot and quantitative slot-blot analyses in the foliar leaves of embryos that had imbibed for 12 to 18 h and leaves of developing seedlings grown either in total darkness or under continuous white light for up to 14 d after imbibition. Both ALA dehydratase and PBG deaminase mRNAs were detectable in embryonic leaves, whereas mRNA encoding the NADPH:protochlorophyllide reductase was not observed at this early developmental stage. All three gene products were found to increase to approximately the same extent in the primary leaves of pea seedlings during the first 6 to 8 d after imbibition (postgermination) regardless of whether the plants were grown in darkness or under continuous white-light illumination. In the leaves of dark-grown seedlings, the highest levels of message accumulation were observed at approximately 8 to 10 d postgermination, and, thereafter, a steady decline in mRNA levels was observed. In the leaves of light-grown seedlings, steady-state levels of mRNA encoding the three chlorophyll biosynthetic enzymes were inversely correlated with leaf age, with youngest, rapidly expanding leaves containing the highest message levels. A corresponding increase in the three enzyme protein levels was also found during the early stages of development in the light or darkness; however, maximal accumulation of protein was delayed relative to peak levels of mRNA accumulation. We also found that although protochlorophyllide was detectable in the leaves immediately after imbibition, the time course of accumulation of the phototransformable form of the molecule coincided with NADPH:protochlorophyllide reductase expression. In studies in which dark-grown seedlings of various ages were subsequently transferred to light for 24 and 48 h, the effect of light on changes in steady-state mRNA levels was found to be more pronounced at later developmental stages. These results suggest that the expression of these three genes and likely those genes encoding other chlorophyll biosynthetic pathway enzymes are under the control of a common regulatory mechanism. Furthermore, it appears that not light, but rather as yet unidentified endogenous factors, are the primary regulatory factors controlling gene expression early in leaf development.

THE FORMATION OF A SHORT-WAVELENGTH CHLOROPHYLLIDE FORM AT PARTIAL PHOTOTRANSFORMATION OF PROTOCHLOROPHYLLIDE IN ETIOPLAST INNER MEMBRANES

Abstract— Partial phototransformation caused the formation and successive accumulation of spectral forms of chlorophyllide in etioplast inner membrane fractions from wheat (Triticum aestivum L. cv. Walde), Low‐temperature fluorescence emission and circular dichroism spectra showed the formation of two chlorophyllide forms with emission maxima at 683 and 694 nm, respectively. The light dependent accumulation of the two forms differed in prolamellar body (PLB) and prothylakoid (PT) fractions. The 694 nm form was preferentially found in PLB fractions which before irradiation were characterized by a 657 nm emitting protochlorophyllide form and a regular PLB structure. The 683 nm form accumulated to a higher extent in PT fractions which before irradiation contained mainly shortwavelength protochlorophyllide forms. The results indicate that at least two photoactive protochlorophyllide forms must be considered. The major part of protochlorophyllide, with a fluoresence emission maximum at 657 nm, is transformed to the 694 nm emitting chlorophyllide first at high light doses. A minor part, revealed only after Gaussian resolution, has a fluorescence emission maximum at 645 nm and was transformed by low light doses to chlorophyllide emitting at 683 nm.

Proteomic analysis of highly purified prolamellar bodies reveals their significance in chloroplast development

The prolamellar body (PLB) proteome of dark-grown wheat leaves was characterized. PLBs are formed not only in etioplasts but also in chloroplasts in young developing leaves during the night, yet their function is not fully understood. Highly purified PLBs were prepared from 7-day-old dark-grown leaves and identified by their spectral properties as revealed by low-temperature fluorescence spectroscopy. The PLB preparation had no contamination of extra-plastidal proteins, and only two envelope proteins were found. The PLB proteome was analysed by a combination of 1-D SDS-PAGE and nano-LC FTICR MS. The identification of chlorophyll synthase in the PLB fraction is the first time this enzyme protein was found in extracts of dark-grown plants. This finding is in agreement with its previous localization to PLBs using activity studies. NADPH:protochlorophyllide oxidoreductase A (PORA), which catalyses the reduction of protochlorophyllide to chlorophyllide, dominates the proteome of PLBs. Besides the identification of the PORA protein, the PORB protein was identified for the first time in dark-grown wheat. Altogether 64 unique proteins, representing pigment biosynthesis, photosynthetic light reaction, Calvin cycle proteins, chaperones and protein synthesis, were identified. The in number of proteins’ largest group was the one involved in photosynthetic light reactions. This fact strengthens the assumption that the PLB membranes are precursors to the thylakoids and used for the formation of the photosynthetic membranes during greening. The present work is important to enhance our understanding of the significance of PLBs in chloroplast development.

POR hits the road: import and assembly of a plastid protein

The biosynthesis of chlorophyll is a strictly light-dependent multistep process in higher plants. The light-dependent step is catalysed by NADPH:protochlorophyllide oxidoreductase (POR, EC.1.6.99.1), which reduces protochlorophyllide (Pchlide) to chlorophyllide (Chlide). POR is nucleus-encoded and post-translationally imported into plastids. It has been proposed that the import of a POR protein isozyme (PORA) is totally dependent on Pchlide and uses a novel import pathway. This proposal is based on findings that PORA import only occurs in the presence of Pchlide and that the presence of overexpressed precursor of Rubisco small subunit (pSS), a protein which is known to use the general import pathway, does not outcompete PORA import. Another study demonstrated that POR precursor protein (pPOR) can be cross-linked to one of the components in the translocation machinery, Toc75, in the absence of Pchlide, and that its import can be outcompeted by the addition of the pSS. This indicates that pSS and pPOR may use the same translocation mechanism. Thus, POR does not necessarily need Pchlide for import – which is in contrast to earlier observations – and the exact POR import mechanism remains unresolved. Once in the stroma, the POR transit peptide is cleaved off and the mature POR protein is associated to the plastid inner membranes. Formation of the correct membrane–associated, thermolysin-protected assembly is strictly dependent of NADPH. As a final step, the formation of the NADPH-Pchlide-POR complex occurs. When POR accumulates in the membranes of proplastids, an attraction of monogalactosyl diacylglycerol (MGDG) can occur, leading to the formation of prolamellar bodies (PLBs) and the development of etioplasts in darkness.

PHOTOTRANSFORMATION OF AGGREGATED FORMS OF PROTOCHLOROPHYLLIDE IN ISOLATED ETIOPLAST INNER MEMBRANES

Abstract— Irradiation of an etioplast inner membrane fraction caused the transformation of two photoactive spectroscopically different protochlorophyllide forms into two chlorophyllide forms. A weak light flash, 6% of a saturating flash, preferentially caused the formation of a short wavelength chlorophyllide form absorbing at 672 nm and emitting at 676 nm. A saturating flash resulted in the formation of the 684 nm absorbing form of chlorophyllide with an emission maximum at 698 nm.

Analysis of the 77 K fluorescence emission and excitation spectra of isolated etioplast inner membranes

Abstract Low-temperature fluorescence emission and excitation analyses revealed four discrete spectral forms of protochlorophyllide and/or protochlorophyll in the etioplast inner membranes isolated from leaves of wheat (Triticum aestivum cv. Kosack). The emission spectra were recorded with various excitation wavelengths between 400 and 500 nm. The excitation spectra were recorded for fluorescence emitted at various wavelengths between 620 and 740 nm. The spectra were analysed by a combination of various computer-aided methods including differential analysis, gaussian resolution and topological plots. The four pigment forms can be characterized by their different excitation (Ex) and emission (Em) bands. Adding the satellite bands of the Qy(0-0) transitions, the emission vibrational (Emv) bands, with correlated amplitudes gave the following delineation: Ex436-Em633-Emv690, Ex443-Em645-Emv711, Ex451-Em657-Emv727, and Ex463-Em671-Emv740. The differences in the spectral properties of the four pigment forms are discussed in relation to possible differences in their structural arrangement, association to NADPH-protochlorophyllide oxidoreductase and localization in prolamellar bodies and/or prothylakoids.

The two spectroscopically different short wavelength protochlorophyllide forms in pea epicotyls are both monomeric

The spectral properties of the protochlorophyllide forms in the epicotyls of dark-grown pea seedlings have been studied in a temperature range, from 10 to 293 K with conventional fluorescence emission and excitation spectroscopy as well as by fluorescence line narrowing (FLN) at cryogenic temperatures. The conventional fluorescence techniques at lower temperatures revealed separate bands at 628, 634-636, 644 and 655 nm. At room temperature (293 K) the 628 and 634-636 nm emission bands strongly overlapped and the band shape was almost independent of the excitation wavelength. Under FLN conditions, vibronically resolved fluorescence spectra could be measured for the 628 and 634-636 nm bands. The high resolution of this technique excluded the excitonic nature of respective excited states and made it possible to determine the pure electronic (0,0) range of the spectra of the two components. Thus it was concluded that the 628 and 634-636 nm (0,0) emission bands originate from two monomeric forms of protochlorophyllide and the spectral difference is interpreted as a consequence of environmental effects of the surrounding matrix. On the basis of earlier results and the data presented here, a model is discussed in which the 636 nm form is considered as an enzyme-bound protochlorophyllide and the 628 nm form as a protochlorophyllide pool from which the substrate is replaced when the epicotyl is illuminated with continuous light.

Light-induced linear dichroism in photoreversibly photochromic sensor pigments 2. Chromophore rotation in immobilized phytochrome

‐Large phytochrome immobilized via anti‐phytochrome immunoglobulin bound to Sepharose beads was irradiated to saturation with unpolarized far‐red light. The apparent absorbance level was recorded in a dual wavelength spectrophotometer with both measuring beams set to either 660 or 730 nm and polarized perpendicular to each other. The sample was then irradiated with red polarized light. The apparent change in absorbance obtained after this irradiation indicated that purified phytochrome could show linear dichroism. From the absorbance values obtained it was computed that the direction of the long‐wavelength transition moment changes by either 32 or 148o, when phytochrome is transformed from Pr to Pfr. Considering the model of Hahn and Song (1981) the latter value appears more likely. In light of these results, the conclusions drawn from in vivo experiments on action dichroism in Dryopteris (Etzold, 1965), Adiantum (Kadota et al., 1982) and Mougeoutia (Haupt. 1970), which point to a 90o rotation. should be reconsidered.