Christian Rueckert
Bielefeld University
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Featured researches published by Christian Rueckert.
International Journal of Systematic and Evolutionary Microbiology | 2011
Rainer Borriss; Xiao-Hua Chen; Christian Rueckert; Jochen Blom; Anke Becker; Birgit Baumgarth; Ben Fan; Rüdiger Pukall; Peter Schumann; Cathrin Spröer; Helmut Junge; Joachim Vater; Alfred Pühler; Hans-Peter Klenk
The whole-genome-sequenced rhizobacterium Bacillus amyloliquefaciens FZB42(T) (Chen et al., 2007) and other plant-associated strains of the genus Bacillus described as belonging to the species Bacillus amyloliquefaciens or Bacillus subtilis are used commercially to promote the growth and improve the health of crop plants. Previous investigations revealed that a group of strains represented a distinct ecotype related to B. amyloliquefaciens; however, the exact taxonomic position of this group remains elusive (Reva et al., 2004). In the present study, we demonstrated the ability of a group of Bacillus strains closely related to strain FZB42(T) to colonize Arabidopsis roots. On the basis of their phenotypic traits, the strains were similar to Bacillus amyloliquefaciens DSM 7(T) but differed considerably from this type strain in the DNA sequences of genes encoding 16S rRNA, gyrase subunit A (gyrA) and histidine kinase (cheA). Phylogenetic analysis performed with partial 16S rRNA, gyrA and cheA gene sequences revealed that the plant-associated strains of the genus Bacillus, including strain FZB42(T), formed a lineage, which could be distinguished from the cluster of strains closely related to B. amyloliquefaciens DSM 7(T). DNA-DNA hybridizations (DDH) performed with genomic DNA from strains DSM 7(T) and FZB42(T) yielded relatedness values of 63.7-71.2 %. Several methods of genomic analysis, such as direct whole-genome comparison, digital DDH and microarray-based comparative genomichybridization (M-CGH) were used as complementary tests. The group of plant-associated strains could be distinguished from strain DSM 7(T) and the type strain of B. subtilis by differences in the potential to synthesize non-ribosomal lipopeptides and polyketides. Based on the differences found in the marker gene sequences and the whole genomes of these strains, we propose two novel subspecies, designated B. amyloliquefaciens subsp. plantarum subsp. nov., with the type strain FZB42(T) ( = DSM 23117(T) = BGSC 10A6(T)), and B. amyloliquefaciens subsp. amyloliquefaciens subsp. nov., with the type strain DSM 7(T)( = ATCC 23350(T) = Fukumoto Strain F(T)), for plant-associated and non-plant-associated representatives, respecitvely. This is in agreement with results of DDH and M-CGH tests and the MALDI-TOF MS of cellular components, all of which suggested that the ecovars represent two different subspecies.
Journal of Bacteriology | 2011
Ben Niu; Christian Rueckert; Jochen Blom; Qi Wang; Rainer Borriss
The genome of Paenibacillus polymyxa M-1 consisted of a 5.8-Mb chromosome and a 360-kb plasmid. Nine sites were dedicated to nonribosomal synthesis of lipopeptides and polyketides. Eight of them were located at the chromosome, while one gene cluster predicted to encode an unknown secondary metabolite was present on the plasmid.
Journal of Bacteriology | 2012
Kun Hao; Pengfei He; Jochen Blom; Christian Rueckert; Zichao Mao; Yixin Wu; Yueqiu He; Rainer Borriss
The genome of rhizobacterium Bacillus amyloliquefaciens subsp. plantarum YAU B9601-Y2 was 4.24 Mb in size and harbored 3,991 coding sequences (CDS). Giant gene clusters were dedicated to nonribosomal synthesis of antimicrobial lipopeptides and polyketides. Remarkably, CAU B946 possessed a gene cluster involved in synthesis of mersacidin.
Journal of Bacteriology | 2012
Jochen Blom; Christian Rueckert; Ben Niu; Qi Wang; Rainer Borriss
The genome of the rhizobacterium Bacillus amyloliquefaciens subsp. plantarum CAU B946 was 4.02 Mb in size and harbored 3,823 genes (coding sequences [CDS]). Nine giant gene clusters were dedicated to nonribosomal synthesis of antimicrobial compounds. Remarkably, strain CAU B946 possessed a gene cluster involved in synthesis of iturin A.
Genome Announcements | 2013
Huijun Wu; Junqing Qiao; Jochen Blom; Christian Rueckert; Oleg N. Reva; Xuewen Gao; Rainer Borriss
ABSTRACT The genome of rhizobacterium Bacillus amyloliquefaciens subsp. plantarum strain NAU-B3 is 4,196,170 bp in size and harbors 4,001 genes. Nine giant gene clusters are dedicated to the nonribosomal synthesis of antimicrobial lipopeptides and polyketides. Remarkably, NAU_B3 contains a large inversion within the central portion of the genome.
Methods in Microbiology | 2011
Rainer Borriss; Christian Rueckert; Jochen Blom; Oliver Bezuidt; Oleg N. Reva; Hans-Peter Klenk
This chapter is devoted to the application of whole genome sequence comparisons in taxonomy. In principle there are three basic approaches: (1) genome indexes, increasingly used as an ‘in silico’ alternative to the experimental DNA–DNA hybridization (DDH); (2) gene content, its successful application depends on the number of genome sequences available for this analysis; and (3) multiple aligned (gene) sequence datasets. In this chapter, we have presented several methods that are practicable for the non-experienced scientist with a background in microbial taxonomy for estimating those genomic parameters. We are sure that further development in the field will facilitate use of genomics as an essential part of prokaryote taxonomy.
BMC Microbiology | 2013
Ben Niu; Joachim Vater; Christian Rueckert; Jochen Blom; Maik J. Lehmann; Jin-Jiang Ru; Xiao-Hua Chen; Qi Wang; Rainer Borriss
Archive | 2012
Zichao Mao; Yixin Wu; Yueqiu He; Rainer Borriss; Kun Hao; Pengfei He; Jochen Blom; Christian Rueckert
Archive | 2012
Rainer Borriss; Jochen Blom; Christian Rueckert; Ben Niu; Qi Wang
Archive | 2012
Rainer Borriss; Ben Niu; Christian Rueckert; Jochen Blom; Qi Wang