Christopher Blake Martin

Riboflavin and UV-Light Based Pathogen Reduction: Extent and Consequence of DNA Damage at the Molecular Level

Abstract We are developing a technology based on the combined application of riboflavin (RB) and light for inactivating pathogens in blood products while retaining the biological functions of the treated cells and proteins. Virus and bacteria reduction measured by tissue culture infectivity or colony formation with UV light alone and in combination with RB yield equivalent results. The effects of RB as a sensitizing agent on DNA in white cells, bacteria and viruses in combination with UV light exposure have been evaluated. UV-mediated DNA degradation in Jurkat T cells and leukocytes in plasma as measured by the FlowTACS assay was significantly increased in the presence of RB. Agarose gel electrophoretic analysis of DNA in Escherichia coli and leukocytes in plasma demonstrated enhanced DNA degradation in the presence of RB. UV light in combination with RB prevented the reactivation of lambda phage compared with samples irradiated in the absence of RB. UV-mediated oxidative damage in calf thymus DNA was also enhanced in the presence of RB. These observations clearly demonstrate that the presence of RB and UV light selectively enhances damage to the guanine bases in DNA. These data also suggest that the type and extent of damage to DNA for virus in the presence of RB and light make it less likely to be repaired by normal repair pathways available in host cells.

Constraining anomalous HVV interactions at proton and lepton colliders

In this paper, we study the extent to which CP parity of a Higgs boson, and more generally its anomalous couplings to gauge bosons, can be measured at the LHC and a future electron-positron collider. We consider several processes, including Higgs boson production in gluon and weak boson fusion and production of a Higgs boson in association with an electroweak gauge boson. We consider decays of a Higgs boson including

An Action Spectrum of the Riboflavin-photosensitized Inactivation of Lambda Phage¶

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Chapter 5:The Antiviral and Antibacterial Properties of Riboflavin and Light: Applications To Blood Safety and Transfusion Medicine

, and

A reduced reaction mechanism for the simulation in ethylene flare combustion

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Validation of a reduced combustion mechanism for light hydrocarbons

. Matrix element approach to three production and decay topologies is developed and applied in the analysis. A complete Monte Carlo simulation of the above processes at proton and

Theoretical study on the relative energies of cationic pterin tautomers

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STAIRSTEP: An interdisciplinary program fo retention and outreach in STEM

colliders is performed and verified by comparing it to an analytic calculation. Prospects for measuring various tensor couplings at existing and proposed facilities are compared.

Work in progress - STAIRSTEP - a program for expanding the student pipeline

Abstract The Action Spectrum of riboflavin (RB) sensitized inactivation of lambda phage was determined between 266 and 575 nm. Below 304 nm, RB depresses the phage reduction by screening phage from radiation that it would otherwise absorb directly. Between 308 and 525 nm, RB sensitizes the inactivation of phage. Enhanced phage reduction is observed at 320 and 500 nm because of binding of RB to the phage and the shifting of the absorption curve of the phage-bound flavin relative to free flavin in phosphate-buffered saline. Enhanced inactivation at 320 and 500 nm and depressed phage inactivation between 360 and 410 nm is also influenced by the inner filter effect.

Combustion mechanism development and CFD simulation for the prediction of soot emission during flaring

In the developed world, blood products are extensively tested for the presence of pathogens prior to administration. Nevertheless, there exists a small, but finite risk of transmission of infectious agents in transfusion medicine.1 The risks of viral infection are due to the “window period”; the per...