Chun-Chuan Lin

High-purity separation of cancer cells by optically induced dielectrophoresis

Abstract. Detecting and concentrating cancer cells in peripheral blood is of great importance for cancer diagnosis and prognosis. Optically induced dielectrophoresis (ODEP) can achieve high resolution and low optical intensities, and the electrode pattern can be dynamically changed by varied light patterns. By changing the projected light pattern, it is demonstrated to separate high-purity gastric cancer cell lines. Traditionally, the purity of cancer cell isolation by negative selection is 0.9% to 10%; by positive selection it is 50% to 62%. An ODEP technology is proposed to enhance the purity of cancer cell isolation to about 77%.

Optically-induced dielectrophoretic technology for cancer cells identification and concentration

The detection and concentration of cancer cells in peripheral blood is of great importance for cancer diagnosis and prognosis. Optically-induced dielectrophoresis (ODEP) can achieve high resolution and low optical intensities, and the electrodes pattern can be dynamically changed by varied light pattern. In this paper, a special lens is used to project the entire image to the ODEP chip to achieve 2.6×2 mm2 manipulating area. By changing projected light pattern, it is demonstrated to separate 10, 20, and 40μm PS (polystyrene) beads; HT-29, 20μm PS beads. The MCF-7 cells concentrated experiments are also demo at 100 μm/sec velocity.

Optically-induced dielectrophoretic technology for cells screening

The ODEP technology has many advantages such as low light energy (~10² W/cm²), virtual electrodes, large manipulating area and rapid screening of cells. In this paper, the 10 μm and 20 μm polystyrene beads are successfully separated by a 40 μm light line width with variable radius circle. Human colon cancer cells (HT-29) concentration experiments are demonstrated and can achieve at the maximum speed about 100 μm/sec.

Optically-induced dielectrophoretic technology for particles manipulation and separation

The optically-induced dielectrophoretic (ODEP) technology has many advantages such as low light energy (~ 102 W/cm2), virtual electrodes, large manipulating area and rapid screening of cells. In this paper, 20 μm polystyrene beads are manipulated by a light circle and concentrated by a variable radius circle. The 10 μm and 40 μm polystyrene beads are successfully separated by a 40 μm light line width with vibrating beveled microfluidic light channels.