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Dive into the research topics where Chung-Yan Koh is active.

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Featured researches published by Chung-Yan Koh.


Chemical Communications | 2013

A universal flow cytometry assay for screening carbohydrate-active enzymes using glycan microspheres

Aarthi Chandrasekaran; Kai Deng; Chung-Yan Koh; Taichi E. Takasuka; Lai F. Bergeman; Brian G. Fox; Paul D. Adams; Anup K. Singh

We describe a simple, multiplexed assay that integrates glycan synthesis, bioconjugation to microspheres, fluorescent chemical/biochemical detection and multiparameter flow cytometric analysis to screen activities of different families of carbohydrate-active enzymes.


Biosensors and Bioelectronics | 2018

Integrated LAMP and immunoassay platform for diarrheal disease detection

Christopher R. Phaneuf; Betty Mangadu; Huu M. Tran; Yooli Kim Light; Anchal Sinha; Frank W. Charbonier; Tyler P. Eckles; Anup K. Singh; Chung-Yan Koh

The challenges of diagnosing infectious disease, especially in the developing world, and the shortcomings of available instrumentation have exposed the need for portable, easy-to-use diagnostic tools capable of detecting the wide range of causative microbes while operating in low resource settings. We present a centrifugal microfluidic platform that combines ultrasensitive immunoassay and isothermal amplification-based screening for the orthogonal detection of both protein and nucleic acid targets at the point-of-care. A disposable disc with automatic aliquoting inlets is paired with a non-contact heating system and precise rotary control system to yield an easy-to-use, field-deployable platform with versatile screening capabilities. The detection of three enterotoxins (cholera toxin, Staphylococcal enterotoxin B, and Shiga-like toxin 1) and three enteric bacteria (C. jejuni, E. coli, and S. typhimurium) were performed independently and shown to be highly sensitive (limit of detection = 1.35-5.50 ng/mL for immunoassays and 1-30 cells for isothermal amplification), highly exclusive in the presence of non-specific targets, and capable of handling a complex sample matrix like stool. The full panel of toxins and bacteria were reliably detected simultaneously on a single disc at clinically relevant sample concentrations in less than an hour. The ability of our technology to detect multiple analyte types in parallel at the point-of-care can serve a variety of needs, from routine patient care to outbreak triage, in a variety of settings to reduce disease impact and expedite effective treatment.


2016 IEEE Healthcare Innovation Point-Of-Care Technologies Conference (HI-POCT) | 2016

Portable centrifugal microfluidic system for diagnostics in resource-limited settings

Christopher R. Phaneuf; Victoria A. VanderNoot; Chung-Yan Koh

The threats of disease outbreaks and exposure to biothreat agents, both accidental and intentional, demand field-deployable technology capable of rapid, sensitive, and accurate diagnosis. In order to address these public health concerns, we present a portable centrifugal microfluidic platform and demonstrate sensitive detection protein antigens, host response antibodies, and nucleic acids down to single digit starting copies. The nucleic acid detection utilizes an isothermal amplification via loop-mediated isothermal amplification (LAMP). The platform, which is composed of a compact optical system for laser induced fluorescence (LIF) detection, a quiet brushless motor, and an efficient non-contact heater, offers an easy-to-use system capable of performing sensitive biodetection in a constrained-resource environment.


TBD | 2014

Enhanced vector borne disease surveillance of California Culex mosquito populations reveals spatial and species-specific barriers of infection.

Sarah S. Wheeler; Ying Fang; Stanley A. Langevin; Owen Soldberg; Victoria A. VanderNoot; Todd W. Lane; William K. Reisen; Deanna Joy Curtis; Chung-Yan Koh; Benjamin H Brodsky

Monitoring infections in vectors such as mosquitoes, sand flies, tsetse flies, and ticks to identify human pathogens may serve as an early warning detection system to direct local government disease preventive measures. One major hurdle in detection is the ability to screen large numbers of vectors for human pathogens without the use of genotype-specific molecular techniques. Next generation sequencing (NGS) provides an unbiased platform capable of identifying known and unknown pathogens circulating within a vector population, but utilizing this technology is time-consuming and costly for vector-borne disease surveillance programs. To address this we developed cost-effective Ilumina® RNA-Seq library preparation methodologies in conjunction with an automated computational analysis pipeline to characterize the microbial populations circulating in Culex mosquitoes (Culex quinquefasciatus, Culex quinquefasciatus/pipiens complex hybrids, and Culex tarsalis) throughout California. We assembled 20 novel and well-documented arboviruses representing members of Bunyaviridae, Flaviviridae, Ifaviridae, Mesoniviridae, Nidoviridae, Orthomyxoviridae, Parvoviridae,


Archive | 2017

METHOD AND APPARATUS FOR PURIFYING NUCLEIC ACIDS AND PERFORMING POLYMERASE CHAIN REACTION ASSAYS USING AN IMMISCIBLE FLUID

Chung-Yan Koh; Yooli Kim Light; Matthew E. Piccini; Anup K. Singh


Archive | 2017

System and method for detecting components of a mixture including a valving scheme for competition assays

Chung-Yan Koh; Matthew E. Piccini; Anup K. Singh


Archive | 2015

A new spin: Adapting SpinDx for Use in Low-Resource Environment.

Melissa Finley; Chung-Yan Koh; Anup K. Singh


Archive | 2015

NON-CONTACT HEATING SYSTEM FOR A CENTRIFUGAL MICROLFUIDIC PLATFORM.

Christopher R. Phaneuf; Yooli Kim Light; Huu Tran; Anup K. Singh; Chung-Yan Koh


Archive | 2013

Pond Crash Forensics: Microbiome Analysis and Field Diagnostics.

Todd W. Lane; Pamela Lane; Chung-Yan Koh; Laura T. Carney; Owen David Solberg


Proposed for publication in Nature Biotechnology. | 2012

Rapid point-of-care diagnostic system for multi-parameter radiation biodosimetry using fingerstick whole blood.

Ulrich Y. Schaff; Chung-Yan Koh; Natalia I. Ossetrova; Gregory Jon Sommer; William F. Blakely

Collaboration


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Anup K. Singh

Sandia National Laboratories

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Gregory Jon Sommer

Sandia National Laboratories

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Todd W. Lane

Sandia National Laboratories

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Laura T. Carney

Sandia National Laboratories

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Matthew E. Piccini

Sandia National Laboratories

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Natalia I. Ossetrova

Uniformed Services University of the Health Sciences

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Pamela Lane

Sandia National Laboratories

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William F. Blakely

Armed Forces Radiobiology Research Institute

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