Claudia Dellavia

Relationship Between the Number of Occlusal Contacts and Masticatory Muscle Activity in Healthy Young Adults

ABSTRACT The electromyographic (EMG) potentials of left and right masseter and temporalis anterior muscles were recorded in 23 healthy young adults during: 1. a 3-second maximum voluntary clench (MVC) on cotton rolls positioned on the posterior teeth (standardized recording); and 2. a 3-second MVC in intercuspal position. EMG potentials recorded in intercuspal position were standardized as a percentage of the mean potentials of the standardized recording, and the EMG muscle activity was calculated. The number of occlusal contacts in intercuspal position was assessed by using eight μm thick shim stocks. Two groups of subjects with either 1. Less than ten occlusal contacts (11 subjects with “few contacts”); or 2. At least ten occlusal contacts (12 subjects with “many contacts”) were selected. The MVC muscle activity in the “few contacts” group was significantly lower than that recorded in the “many contacts” group (p<0.005). In conclusion, the number of occlusal contacts and masticatory muscular function are significantly related, at least in young adults with a sound stomatognathic apparatus.

A quantitative three-dimensional assessment of soft tissue facial asymmetry of cleft lip and palate adult patients.

The three-dimensional coordinates of 23 selected soft-tissue facial landmarks were digitized on 18 cleft lip and palate (CLP) white patients (11 male and 7 female patients aged 19–27 years) and 161 healthy controls (73 female and 89 male subjects aged 18–30 years) by an electromagnetic instrument. Facial asymmetry was quantified by detecting a plane of symmetry and the centers of gravity (CG) of the right and left hemifaces and by calculating the distance between the two CG (distance from symmetry [DFS]). Both absolute (millimeters) and percentage (of the nasion center of gravity distance) DFS was obtained. The asymmetry of single landmarks was also quantified. Overall, asymmetry in operated CLP patients appeared only moderately larger than that measured in the healthy reference population, with the largest value being only 5% larger than the maximum normal asymmetry. Female patients had a somewhat larger lateral asymmetry than male patients, and unilateral CLP patients (particularly the men) were more asymmetrical than bilateral CLP patients. Pronasale and subnasale landmarks were asymmetrical in 8 patients, whereas endocanthion, zygion, cheilion, and gonion landmarks were symmetrical in all patients. In conclusion, the facial soft-tissue structures of CLP patients operated on as adults were only moderately more asymmetrical than those measured in a reference group of the same age, sex, and ethnicity.

Soft tissues around long-term platform switching implant restorations: a histological human evaluation. Preliminary results.

BACKGROUND Switching platform restorations seems to reduce the peri-implant bone resorption and to preserve the peri-implant soft tissues. AIM The aim of the present human study was to compare histologically the peri-implant soft tissue in switching and traditional platform implants 4 years after restoration. MATERIALS AND METHODS Forty-eight months after implant restoration, 37 peri-implant soft tissue samples from 14 patients were harvested from traditionally restored implants (control group) and from three different platforms mismatching 0.25-0.85 mm (test groups). At the harvesting time, all sites were clinically healthy. Samples were processed to evaluate the inflammatory infiltrate area [inflamed connective tissue (ICT)], the microvascular density (MVD) and the collagen content (AA%). RESULTS At the analyses, no significant differences were found between groups in terms of ICT, MVD and AA% (p>0.05). In all groups, most samples with a well-preserved junctional epithelium showed a small and localized inflammatory infiltrated associated with not-well-oriented collagen fibres and an increased MVD. CONCLUSIONS Forty-eight months after restoration, switching and traditional platform implants had similar histological peri-implant soft tissue features, despite different bone level changes detected radiographically and published in a previous parent study. The present study seems to confirm platform switching as a safe prosthetic concept leading to better maintenance of peri-implant bone levels. However, further histological studies are required to longitudinally confirm the present data.

Postural Stability of Athletes in Special Olympics

To assess body equilibrium in athletes with intellectual disability, 60 adults with intellectual disability (30 Down syndrome, 30 nonsyndromic) participating in the 2005 Italian Special Olympics games were tested, and data for 30 healthy control adults were tested. Each subject performed four posturographic tests with open eyes, open eyes and cotton rolls between antagonist teeth, and closed eyes and cotton rolls between teeth. For each subject and test, oscillations of the bodys center of foot pressure on a force platform were measured. Comparisons of the center of foot pressure sway area between groups were computed. Ratios of the sway area for the center of foot pressure among experimental conditions were compared for all athletes. The athletes with Down syndrome had larger sway of center of foot pressure area than controls and smaller than that of athletes for the nonsyndromic. All participants oscillated less with open eyes than with closed eyes. The cotton rolls reduced the sway area for the center of foot pressure by participants, while athletes with intellectual disability showed larger body sway than healthy ones, but cotton rolls between the teeth seemed to improve their postural performance.

Sinus Lift Using a Nanocrystalline Hydroxyapatite Silica Gel in Severely Resorbed Maxillae: Histological Preliminary Study

PURPOSE The aim of this preliminary study was to evaluate histologically a nanocrystalline hydroxyapatite silica gel in maxillary sinus floor grafting in severely resorbed maxillae. MATERIALS AND METHODS A total of 16 consecutive patients scheduled for sinus lift were recruited during this study. Patients were randomly divided in two groups, eight patients each. In both groups, preoperative residual bone level ranged between 1 and 3 mm (mean value of 2.03 mm). No membrane was used to occlude the buccal window. Second surgery was carried out after a healing period of 3 months in Group 1 and 6 months in Group 2. Using a trephine bur, one bone specimen was harvested from each augmented sinus and underwent histological and histomorphometric analysis. RESULTS Histological analysis showed significant new bone formation and remodeling of the grafted material. In the cores obtained at 6 months, regenerated bone, residual NanoBone, and bone marrow occupied respectively 48 +/- 4.63%, 28 +/- 5.33%, and 24 +/- 7.23% of the grafted volume. In the specimens taken 3 months after grafting, mean new bone was 8 +/- 3.34%, mean NanoBone was 45 +/- 5.10%, and mean bone marrow was 47 +/- 6.81% of the bioptical volume. CONCLUSIONS Within the limits of this preliminary prospective study, it was concluded that grafting of maxillary sinus using nanostructured hydroxyapatite silica gel as only bone filler is a reliable procedure also in critical anatomic conditions and after early healing period.

A Quantitative Three-Dimensional Assessment of Abnormal Variations in Facial Soft Tissues of Adult Patients With Cleft Lip and Palate

OBJECTIVE To supply quantitative information about the facial soft tissues of adult operated patients with cleft lip and palate (CLP). DESIGN, SETTING, AND PATIENTS The three-dimensional coordinates of soft tissue facial landmarks were obtained using an electromagnetic digitizer in 18 Caucasian patients with CLP (11 males and 7 females aged 19 to 27 years) and 162 healthy controls (73 females and 89 males aged 18 to 30 years). From the landmarks, 15 facial dimensions and two angles were calculated. Data were compared with those collected in healthy individuals by computing z-scores. Two summary anthropometric measurements for quantifying craniofacial variations were assessed in both the patients and reference subjects: the mean z-score (an index of overall facial size), and its SD, called the craniofacial variability index (an index of facial harmony). RESULTS In treated patients with CLP, facial size was somewhat smaller than in normal individuals, but in all occasions the mean z-score fell inside the normal interval (mean +/- 2 SD). Almost all patients had a craniofacial variability index larger than the normal interval, indicating a global disharmonious appearance. Overall, in patients pronasale, subnasale, and pogonion were more posterior, the nose was shorter and larger, the face was narrower, and the soft tissue profile and upper lip were flatter than in the reference population. CONCLUSIONS The facial soft tissue structures of adult operated patients with CLP differed from those of normal controls of the same age, sex, and ethnic group. In this patient group, surgical corrections of CLP failed to provide a completely harmonious appearance, even if the deviations from the reference were limited. Further analyses of larger groups of patients are needed.

Oral health conditions in Italian Special Olympics athletes.

During three Italian Special Olympics National Games, 365 athletes were screened. Dental and medical conditions and demographic data were recorded. The athletes were divided into two groups: those with Down syndrome (DS) and those without DS but who had intellectual disabilities (non-DS). Most of the subjects were in good systemic health. Total DMFt was 10.3 (SD 5.8; D=1.3; M=6.1; F=2.8). Decayed and filled teeth were significantly more frequent in athletes who did not have DS compared to those with DS. No significant differences were found between the two groups in the number of subjects with filled, sealed, or traumatized teeth. Athletes with DS and without DS who participated in the Italian Special Olympics had a similar oral status, which was better than Italian persons who were institutionalized and who had an intellectual disability.

Nasal dimensions in normal subjects: Conventional anthropometry versus computerized anthropometry

The aim of the current investigation was to compare computerized measurements of nasal linear distances (nasal tip protrusion, height of the cutaneous upper lip, length of the nasal bridge) collected from 705 healthy individuals from Northern Italy (age range 6–60 years) using an electromagnetic digitizer with conventional anthropometric measurements: one set obtained on individuals of central European origin (Zankl et al. [2002: Am J Med Genet 111:388‐391]), and one set collected from North American Caucasians (Farkas et al. [1994: Anthropometry of the Head and Face 241–335]). On average, the present lengths of the nasal bridge were always significantly smaller than the European data (P < 0.01, Students t for independent samples). Nevertheless, only in one sex and age group of 18 the discrepancy between the two mean values was larger than 10 mm. In other 10 groups, the mean values differed less than 5 mm. More limited differences (up to 6.5 mm) were found in comparison to the American data. For nasal tip protrusion, digital and conventional data were significantly different (up to 4 mm) in 18 instances of 30. On average, the present heights of the cutaneous upper lip were always smaller than the European data (up to 2.9 mm). The differences were statistically significant in 14 groups of 18. Minor discrepancies (less than 1 mm in nine groups of 12) were found in comparison to the American data. Statistical significance was reached only in seven comparisons. In conclusion, the conventional anthropometric and the digital data compared in the current study, though not superimposable, seemed sufficiently interchangeable, at least from a clinical point of view.

Role of autologous rabbit adipose‐derived stem cells in the early phases of the repairing process of critical bone defects

Adipose‐derived stem cells (ASCs) may represent a novel and efficient tool to promote bone regeneration. In this study, rabbit ASCs were expanded in culture and used for the regeneration of full‐thickness bone defects in the proximal epiphysis of tibia of 12 New Zealand rabbits. Defects were implanted with graft material as follows: untreated (control), empty hydroxyapatite (HA) disk, ASCs alone, and HA disk seeded with ASCs. Each isolated ASCs population was tested in vitro: they all showed a high proliferation rate, a marked clonogenic ability, and osteogenic differentiation potential. Eight weeks after implantation, macroscopic analyses of all the samples showed satisfactory filling of the lesions without any significant differences in term of stiffness between groups treated with or without cells (p > 0.05). In both the scaffold‐treated groups, a good osteointegration was radiographically observed. Even if HA was not completely reabsorbed, ASCs‐loaded HA displayed a higher scaffold resorption than the unloaded ones. Histological analyses showed that the osteogenic abilities of the scaffold‐treated defects was greater than those of scaffold‐free samples, and in particular new formed bone was more mature and more similar to native bone in presence of ASCs. These results demonstrated that autologous ASCs–HA constructs is a potential treatment for the regeneration of bone defects.

Three-dimensional nasal morphology in cleft lip and palate operated adult patients

Nasal morphology was assessed three-dimensionally in 18 cleft lip and palate operated patients aged 19 to 27 years, and in 161 sex- and age-matched control subjects. Comparisons were performed by Student t and Watson–Williams’ tests. No differences were found in nasal volume or surface. Nasal width, alar base width, and inferior width of the nostrils were significantly larger in male patients than in the reference men. The nasal bridge was shorter. Similar differences were found in the female patients. In addition, the right nostril was larger and the nasal height shorter. In men, the nasolabial and the nasal tip angles were smaller in the patients than in the reference subjects, whereas the facial convexity angle was larger in the patients. In conclusion, the nose of adult operated cleft lip and palate patients differed from that of normal control subjects. Surgical corrections of the cleft lip and palate failed to provide a completely normal appearance. The methods might be used to indicate where additional procedures might be performed to approximate a reference population.