Clovis Daniel Borges

Sistemas de preparo do solo, plantas de cobertura e produtividade da cultura da mandioca

O objetivo deste trabalho foi avaliar os efeitos do uso de plantas de cobertura e de sistemas de preparo do solo, no desenvolvimento e na produtividade da cultura da mandioca (Manihot esculenta Crantz). O trabalho foi conduzido em Argissolo Vermelho, sob sistema convencional de preparo do solo, e em cultivo minimo sobre palhada de mucuna-cinza (Stizolobium cinereum Piper & Tracy), sorgo granifero [Sorghum bicolor (L.) Moench] e milheto [Pennisetum americanum (L.) K. Schum.]. Aos dezoito meses apos o plantio da mandioca, foram avaliados: altura de plantas, producao de massa de materia seca da parte aerea, numero de raizes tuberosas, produtividade, percentagem de materia seca e de amido nas raizes tuberosas e indice de colheita. Observou-se que o sistema convencional de preparo do solo pode ser substituido, na cultura da mandioca, pela pratica do cultivo minimo, associada ao uso de coberturas vegetais, por promover incrementos significativos na produtividade da cultura, especialmente, quando se utiliza o milheto como planta de cobertura. O uso de plantas de cobertura no pre-cultivo de mandioca, em sistema de preparo minimo do solo, representa uma alternativa eficiente para um melhor manejo dessa cultura.

Oxidative mitigation of aquatic methane emissions in large Amazonian rivers

The flux of methane (CH4 ) from inland waters to the atmosphere has a profound impact on global atmospheric greenhouse gas (GHG) levels, and yet, strikingly little is known about the dynamics controlling sources and sinks of CH4 in the aquatic setting. Here, we examine the cycling and flux of CH4 in six large rivers in the Amazon basin, including the Amazon River. Based on stable isotopic mass balances of CH4 , inputs and outputs to the water column were estimated. We determined that ecosystem methane oxidation (MOX) reduced the diffusive flux of CH4 by approximately 28-96% and varied depending on hydrologic regime and general geochemical characteristics of tributaries of the Amazon River. For example, the relative amount of MOX was maximal during high water in black and white water rivers and minimal in clear water rivers during low water. The abundance of genetic markers for methane-oxidizing bacteria (pmoA) was positively correlated with enhanced signals of oxidation, providing independent support for the detected MOX patterns. The results indicate that MOX in large Amazonian rivers can consume from 0.45 to 2.07 Tg CH4 yr(-1) , representing up to 7% of the estimated global soil sink. Nevertheless, climate change and changes in hydrology, for example, due to construction of dams, can alter this balance, influencing CH4 emissions to atmosphere.

Fauna invertebrada e atributos físicos e químicos do solo em sistemas de integração lavoura-pecuária

The objective of this work was to evaluate the effect of integrated crop-livestock (ICL) systems on the main groups of soil invertebrate macrofauna and the relationship of these organisms with soil physical and chemical attributes. The conventional system, integrated crop-livestock system, no-tillage system, continuously cultivated pasture, and natural fragments of Cerrado and of semi-deciduous forest were compared. The experiment was carried out in Dourados, MS, Brazil, on a Typic Hapludox soil, divided into strips. The highest density, richness, and diversity of families were found in the ICL system, under no-tillage, and in the continuously cultivated pasture. The conventional system caused greater reduction of the soil invertebrate community. The ICL system favors the maintenance of the invertebrate fauna diversity, the formation of stable aggregates, and soil fertility. The Sthaphylinidae family is a potential soil quality bioindicator, especially in the semi-deciduous forest and in the ICL system.

Soil bacterial diversity in degraded and restored lands of Northeast Brazil.

Land degradation deteriorates biological productivity and affects environmental, social, and economic sustainability, particularly so in the semi-arid region of Northeast Brazil. Although some studies exist reporting gross measures of soil microbial parameters and processes, limited information is available on how land degradation and restoration strategies influence the diversity and composition of soil microbial communities. In this study we compare the structure and diversity of bacterial communities in degraded and restored lands in Northeast Brazil and determine the soil biological and chemical properties influencing bacterial communities. We found that land degradation decreased the diversity of soil bacteria as indicated by both reduced operational taxonomic unit (OTU) richness and Shannon index. Soils under native vegetation and restoration had significantly higher bacterial richness and diversity than degraded soils. Redundancy analysis revealed that low soil bacterial diversity correlated with a high respiratory quotient, indicating stressed microbial communities. By contrast, soil bacterial communities in restored land positively correlated with high soil P levels. Importantly, however, we found significant differences in the soil bacterial community composition under native vegetation and in restored land, which may indicate differences in their functioning despite equal levels of bacterial diversity.

Atributos físicos e teor de matéria orgânica na camada superficial de um argissolo vermelho cultivado com mandioca sob diferentes manejos

The maintenance and improvement of soil quality are crucial to the stability, sustainability and productivity of natural ecosystems and agroecosystems. The understanding and quantification of the impact of tillage systems in their physical quality are fundamental in the development of sustainable agricultural systems. This study was carried out to evaluate the effects of management practices on physical properties and organic matter content of an Ultisol cultivated with cassava following the use of distinct cover crops. A field experiment was carried out in Gloria de Dourados, Mato Grosso do Sul State, Brazil, in a sandy texture Ultisol. The following management practices were used: conventional tillage (SC), no-tillage system with cultural residues of Estilozobium pruriens (PDMu), Sorghum bicolor (PDSo) or Pennisetum glaucum (PDMi). A native vegetation (VN) soil was used as reference for comparison. Evaluations were performed in May 2003 (after planting cassava) and August 2004. Organic matter contents in the different soil management practices were similar. The effects of these systems on the soil physical properties were more pronounced in the early phase of cassava establishment. Aggregate stability was higher in cultivation systems that used grass as cover crop. Bulk density was lower and total soil porosity, particularly macroporosity, was higher in the conventional when compared to the no-tillage system using cover crops.

Soil microbial community dynamics and assembly under long-term land use change

ABSTRACT We evaluated the bacterial and archaeal community dynamics and assembly in soils under forest, grassland and no‐till cropping, using a high‐throughput shotgun metagenomics approach. No significant alterations in alpha diversity were observed among different land uses, but beta diversity in grassland was lower than that observed in forest and no‐till soils. Grassland communities showed assembly that predominantly followed the neutral model, i.e. high homogenizing selection with moderate dispersion, leading to biotic homogenization. Both no‐till and forest soil communities were found to have assembly that predominantly followed a niche model, i.e. low rates of dispersal and weak homogenizing selection, resulting in maintenance of higher beta diversity relative to grasslands, indicating niche specialization or variable selection. Taken together, our results indicate that the patterns of assembly and their governing processes are dependent on the land use employed after deforestation, with consequences for taxa turnover and microbial functional potential.

New Biological Insights Into How Deforestation in Amazonia Affects Soil Microbial Communities Using Metagenomics and Metagenome-Assembled Genomes

Deforestation in the Brazilian Amazon occurs at an alarming rate, which has broad effects on global greenhouse gas emissions, carbon storage, and biogeochemical cycles. In this study, soil metagenomes and metagenome-assembled genomes (MAGs) were analyzed for alterations to microbial community composition, functional groups, and putative physiology as it related to land-use change and tropical soil. A total of 28 MAGs were assembled encompassing 10 phyla, including both dominant and rare biosphere lineages. Amazon Acidobacteria subdivision 3, Melainabacteria, Microgenomates, and Parcubacteria were found exclusively in pasture soil samples, while Candidatus Rokubacteria was predominant in the adjacent rainforest soil. These shifts in relative abundance between land-use types were supported by the different putative physiologies and life strategies employed by the taxa. This research provides unique biological insights into candidate phyla in tropical soil and how deforestation may impact the carbon cycle and affect climate change.

Biofilm formation and antimicrobial sensitivity of lactobacilli contaminants from sugarcane-based fuel ethanol fermentation

Industrial ethanol fermentation is subject to bacterial contamination that causes significant economic losses in ethanol fuel plants. Chronic contamination has been associated with biofilms that are normally more resistant to antimicrobials and cleaning efforts than planktonic cells. In this study, contaminant species of Lactobacillus isolated from biofilms (source of sessile cells) and wine (source of planktonic cells) from industrial and pilot-scale fermentations were compared regarding their ability to form biofilms and their sensitivity to different antimicrobials. Fifty lactobacilli were isolated and the most abundant species were Lactobacillus casei, Lactobacillus fermentum and Lactobacillus plantarum. The majority of the isolates (87.8%) were able to produce biofilms in pure culture. The capability to form biofilms and sensitivity to virginiamycin, monensin and beta-acids from hops, showed inter- and intra-specific variability. In the pilot-scale fermentation, Lactobacillus brevis, L. casei and the majority of L. plantarum isolates were less sensitive to beta-acids than their counterparts from wine; L. brevis isolates from biofilms were also less sensitive to monensin when compared to the wine isolates. Biofilm formation and sensitivity to beta-acids showed a positive and negative correlation for L. casei and L. plantarum, respectively.