Cristiane Scavuzzi Moura

Avaliação histomorfométrica do parênquima testicular de ratos adultos tratados com diferentes doses de ivermectina

The aim of this work was to evaluate the ivermectin effect on the testicular parenchyma through the daily spermatic production and the efficiency of the spermatogenesis in adult Wistar rats treated with different dosages (200, 400 and 600µg/kg) of ivermectin. Based on the histomorfometric evaluation, ivermectin had no deleterious effect on the testicular parenchyma and spermatogesis, which one was confirmed through the maintenance of the daily spermatic input and intrinsic income of spermatogenesis (PED/g/t), as well as by the maintenance of the testicular parenchyma structure. Based on the quantitative and qualitative results of spermatogenesis, it is possible to conclude that ivermectin does not have toxic-degenerative effect on the testicular parenchyma of adult Wistar rats.

Efeito da temperatura de descongelação na integridade de espermatozoides criopreservados de cães

Aiming to evaluate the influence of the thawing temperature on the viability of canine cryopreserved sperm, Basset Hound (n=3) and Rottweiler (n=3) dogs were used, submitted to semen collected through manual manipulation. Semen samples were thawed at 37oC during 1min (G1) or at 70oC during 6s (G2), and evaluated for progressive motility, vigor and acrosome integrity, after 0, 30 e 60 minutes of incubation (37oC), and sperm ultrastructure immediately after thawing. In all incubation times, the average of progressive motility was higher (P 0.05) between groups, and the percentage of gametes with intact acrosome was higher (P<0.05) on sperm cells from G1 than from G2. Ultrastructural changes were identified on dog sperm from both groups, and were observed in higher quantity in gametes from G2 Group. It can be concluded that samples of frozen dog sperm must be thawed at 37°C for 1min.

Activity of crude extract of the sexual renal segment from Crotalus durissus on spermatic kinematics of thawed dog semen

Background Males of the order Squamata have similar structure to the seminal vesicles of mammals called Renal Sexual Segment (RSS). The RSS regions are hypertrophied kidney ducts, androgen-dependent, responsible for providing secretions (complex of glycogen, mucopolysaccharides, mucoproteins and lipids) that nourishes and keeps the sperm during copulation [1,3], acting accessorily on reproductive cycle, once when mixed with sperm, and nutrient function, also act as activating agent of sperm transferred to the cloaca in the female during intercourse [2,3]. Valverde (personal communication, April 6, 2010) tested the crude extract of the renal sexual segment Crotalus durissus in bovine semen and the results showed a significant increase in sperm motility and vigor of the samples, but this activity was lost over time storage of the extract. The promising data in this study aimed to evaluate the effect of the addition of crude extract of the RSS from C. durissus on spermatic kinematics of thawed dog semen.