Cristiano Schiavinato Baldan

Treatment time of ultrasound therapy interferes with the organization of collagen fibers in rat tendons

BACKGROUND The application time of therapeutic ultrasound is an infrequently studied dosimetric variable that affects tissue repair. OBJECTIVES The aim of this study was to evaluate the effects of different treatment times of therapeutic ultrasound (US) on the organization of collagen fibers in the tendons of rats. METHOD Forty Wistar rats were selected (300±45 g), and the rats were divided into five groups (n=8 for each group): Control, without tenotomy or any treatment; tenotomy group, with tenotomy and without treatment; US groups (US1, US2, and US3), subjected to tenotomy and treated with US for one, two, or three minutes per area of the transducer, respectively. The animals were sacrificed on the 12th post-operative day, and the tendons were surgically removed for analyses of the collagen fiber organization by means of birefringence analysis. RESULTS The collagen fibers exhibited better aggregation and organization in the US3 group compared with the tenotomy group (p<0.05). CONCLUSIONS The findings suggest that US applied for three minutes per treated area improves the organization of collagen fibers during rat tendon repair.

The effects of different doses of 670 nm diode laser on skin flap survival in rats

PURPOSE To investigate the effects of different low-level laser therapy (LLLT) doses on random skin flap rats. METHODS Forty Wistar rats were randomly divided in four groups. The control group (CG) was not irradiated. The experimental groups were irradiated with a diode laser 670 nm with different energies per point: group 2 (G2) with 0.06 J; group 3 (G3) 0.15 J and group 4 (G4) 0.57 J. The three groups were irradiated in 12 equally distributed points in the cranial skin flap portion. They were submitted to the irradiation during the immediate, first and second postoperative days. The necrosis area was evaluated in the seventh postoperative day. RESULTS The CG shows 49.35% of necrosis area in the skin flap; G2, 39.14%; G3, 47.01% and G4, 29.17% respectively. There was a significantly difference when G4 was compared with CG`s skin flap necrosis area. CONCLUSION The low-level laser therapy diode 670 nm with 0.57 J energy per point increases the survival in randomic skin flap rats.

Conhecimento e envolvimento de graduandos em fisioterapia acerca dos preceitos éticos da experimentação animal

Knowledge and involvement on ethical principles of animal experimentation in undergraduate physiotherapy This article analyzes a sample of university students from the Physiotherapy Course, the indicators of ethical use of animals in research and teaching to the level of knowledge and interest of the students on the topic of animal ethics. A questionnaire was distributed to the students of the first and second semesters of Physiotherapy, which was filled out in the classroom voluntarily. Data was analyzed and a descriptive discussion took place subsequently. The questionnaire was completed by 193 students. Among the surveyed students, the majority had prior knowledge on the topic of animal ethics, but never attended classes with practical use of animals and claimed they have never thought about replacing animals by alternative methods. Students were sensitized on the number of animals used in teaching and research and demonstrated knowledge of the subject.

Knowledge and involvement on ethical principles of animal experimentation in undergraduate physiotherapy

Knowledge and involvement on ethical principles of animal experimentation in undergraduate physiotherapy This article analyzes a sample of university students from the Physiotherapy Course, the indicators of ethical use of animals in research and teaching to the level of knowledge and interest of the students on the topic of animal ethics. A questionnaire was distributed to the students of the first and second semesters of Physiotherapy, which was filled out in the classroom voluntarily. Data was analyzed and a descriptive discussion took place subsequently. The questionnaire was completed by 193 students. Among the surveyed students, the majority had prior knowledge on the topic of animal ethics, but never attended classes with practical use of animals and claimed they have never thought about replacing animals by alternative methods. Students were sensitized on the number of animals used in teaching and research and demonstrated knowledge of the subject.

El conocimiento y la participación en los principios éticos de experimentación animal de los graduandos en fisioterapia

Knowledge and involvement on ethical principles of animal experimentation in undergraduate physiotherapy This article analyzes a sample of university students from the Physiotherapy Course, the indicators of ethical use of animals in research and teaching to the level of knowledge and interest of the students on the topic of animal ethics. A questionnaire was distributed to the students of the first and second semesters of Physiotherapy, which was filled out in the classroom voluntarily. Data was analyzed and a descriptive discussion took place subsequently. The questionnaire was completed by 193 students. Among the surveyed students, the majority had prior knowledge on the topic of animal ethics, but never attended classes with practical use of animals and claimed they have never thought about replacing animals by alternative methods. Students were sensitized on the number of animals used in teaching and research and demonstrated knowledge of the subject.