Cristina Pagani
University of Milan
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BMC Infectious Diseases | 2017
Sara Giordana Rimoldi; Bernardina Gentile; Cristina Pagani; Annamaria Di Gregorio; Anna Anselmo; Anna Maria Palozzi; Antonella Fortunato; Valentina Pittiglio; Anna Lisa Ridolfo; Maria Rita Gismondo; Giuliano Rizzardini; Florigio Lista
BackgroundThe emergence of carbapenem-resistant Klebsiella pneumoniae strains is threatening antimicrobial treatment.MethodsSixty-eight carbapenemase-producing K. pneumoniae strains isolated at Luigi Sacco University Hospital-ASST Fatebenefratelli Sacco (Milan, Italy) between 2012 and 2014 were characterised microbiologically and molecularly. They were tested for drug susceptibility and carbapenemase phenotypes, investigated by means of repetitive extra-genic palindromic polymerase chain reaction (REP-PCR), and fully sequenced by means of next-generation sequencing for the in silico analysis of multi-locus sequence typing (MLST), their resistome, virulome and plasmid content, and their core single nucleotide polymorphism (SNP) genotypes.ResultsAll of the samples were resistant to carbapenems, other β-lactams and ciprofloxacin; many were resistant to aminoglycosides and tigecycline; and seven were resistant to colistin. Resistome analysis revealed the presence of blaKPC genes and, less frequently blaSHV, blaTEM, blaCTX-M and blaOXA, which are related to resistance to carbapenem and other β-lactams. Other genes conferring resistance to aminoglycoside, fluoroquinolone, phenicol, sulphonamide, tetracycline, trimethoprim and macrolide-lincosamide-streptogramin were also detected. Genes related to AcrAB-TolC efflux pump-dependent and pump-independent tigecycline resistance mechanisms were investigated, but it was not possible to clearly correlate the genomic features with tigecycline resistance because of the presence of a common mutation in susceptible, intermediate and resistant strains. Concerning colistin resistance, the mgrB gene was disrupted by an IS5-like element, and the mobile mcr-1 and mcr-2 genes were not detected in two cases. The virulome profile revealed type-3 fimbriae and iron uptake system genes, which are important during the colonisation stage in the mammalian host environment. The in silico detected plasmid replicons were classified as IncFIB(pQil), IncFIB(K), ColRNAI, IncX1, IncX3, IncFII(K), IncN, IncL/M(pMU407) and IncFIA(HI1). REP-PCR showed five major clusters, and MLST revealed six different sequence types: 512, 258, 307, 1519, 745 and 101. Core SNP genotyping, which led to four clusters, correlated with the MLST data. Isolates of the same sequencing type often had common genetic traits, but the SNP analysis allowed greater strain tracking and discrimination than either the REP-PCR or MLST analysis.ConclusionOur findings support the importance of implementing bacterial genomics in clinical medicine in order to complement traditional methods and overcome their limited resolution.
Journal of Infection and Public Health | 2017
Sara Giordana Rimoldi; Cristina Pagani; Erika Longhi; Valentina Di Cristo; Annamaria Di Gregorio; Alessandro Mancon; Pietro Zerbi; Cristina Gervasoni; Maria Rita Gismondo; Agostino Riva
One of the most important Staphylococcus aureus virulence factors is Panton-Valentine leukocidin (PVL). We describe an outbreak of recurrent cutaneous PVL infections in different members of three family clusters. Molecular investigations were performed to confirm the presence of the mecA and PVL genes and to assign the SCCmec type, sequence type (ST) and clonal relatedness. A strain of PVL-producing methicillin-resistant S. aureus (MRSA) was responsible for infection in two related families (A and B), and a third family (C) was infected with PVL-producing methicillin-sensitive S. aureus (MSSA). Molecular investigations revealed the same clone of community-acquired (CA)-MRSA, PVL positive ST8, and SCCmec IV in families A and B and CA-MSSA PVL positive ST15 in family C. S. aureus PVL may give rise to recurrent uncontrolled infections that are difficult to eradicate, and close family contacts are at high risk for transmission.
International Journal of One Health | 2017
Sara Giordana Rimoldi; Annamaria Di Gregorio; V. Sala; Eleonora De Faveri; Cristina Pagani; Pietro Olivieri; Claudio Savi; Anna Lisa Ridolfo; Antona Carlo; Maria Rita Gismondo
Aim: Methicillin-resistant Staphylococcus aureus remains a leading cause of hospital and community infections. We report a retrospective molecular characterization of S. aureus strains from different settings: hospital workers and patients, and veterinarian surgeons and pets. Materials and Methods: Eighty-nine S. aureus isolates obtained from nasal swabs of 10 patients, 17 health-care workers (HCWs), 9 pets, and 53 veterinarians were genotypically characterized by means of repetitive extragenic palindromic polymerase chain reaction (Rep PCR) and whole-genome sequencing. Results: Thirteen different sequence types (STs) were detected: ST398, ST22, ST8, ST30, ST15, ST5, ST121, ST45, ST10, ST6, ST34, ST97, and ST1. Two new STs differing from ST22 and ST5 for a single multilocus sequence typing gene were also identified. Rep PCR documented a genetic relationship among isolates obtained from 5 veterinarians and 10 HCWs. Conclusion: The large diversity of S. aureus strains detected may reflect a larger epidemiology within the hospital and community, in which companion animals likely act as a reservoir. We identified the circulation of ST5, ST8, ST15, ST22, ST30, ST45, and ST121 both in the hospital and veterinarian environment. Starting from the idea of a unique setting where our population lives, we consider the relationship between community- and hospital-acquired S. aureus.
International Journal of Clinical & Medical Microbiology | 2016
Sara Giordana Rimoldi; Cristina Pagani; Annamaria Di Gregorio; Alessandro Palazzin; Pietro Romano; Stefania Piconi; Simone Passerini; Giuliano Salvadori del Prato; Massimo Coen; Erika Longhi; Maria Rita Gismondo
Pasteurella spp is the most frequently pathogen isolated from infected wound inflicted by domestic animal bites. Here we present a case of isolation of Pasteurella pneumotropica like organism from knee mega prosthesis infection occurred in 63 years old female.
Archives of Virology | 2011
Sara Giordana Rimoldi; Fabrizio Stefani; Cristina Pagani; Lisa Lucia Chenal; Nadia Zanchetta; Ilaria Di Bartolo; Alessandra Lombardi; Franco Maria Ruggeri; Dario Di lillo; Gian Vincenzo Zuccotti; Maria Rita Gismondo
Journal of Infection and Public Health | 2016
Anna Lisa Ridolfo; Sara Giordana Rimoldi; Cristina Pagani; Andrea F. Marino; Anna Piol; Matteo Rimoldi; Pietro Olivieri; Massimo Galli; Lucia Dolcetti; Maria Rita Gismondo
The Annals of Thoracic Surgery | 2016
Sara Giordana Rimoldi; Elena De Vecchi; Cristina Pagani; Agostino Zambelli; Annamaria Di Gregorio; Enrica Bosisio; Paolo Vanelli; Roberto Scrofani; Maria Rita Gismondo; Giovanni Cagnoni; Carlo Antona
Archive | 2017
Sara Giordana Rimoldi; Bernardina Gentile; Cristina Pagani; Annamaria Di Gregorio; Anna Anselmo; Anna Maria Palozzi; Antonella Fortunato; Valentina Pittiglio; Anna Lisa Ridolfo; Maria Rita Gismondo; Giuliano Rizzardini; Florigio Lista
Società Italiana di Patologia e Allevamento dei Suini | 2014
E. De Faveri; Sara Giordana Rimoldi; Cristina Pagani; V. Sala
Microbiologia Medica | 2011
Lisa Lucia Chenal; Alessandra Lombardi; Nadia Zanchetta; Giuseppe Giuliani; Valeria Micheli; Sara Giordana Rimoldi; Loredana Tocalli; Cristina Pagani; Maria Rita Gismondo