Cyril Hrnčár

Effect of Lactobacillus fermentum and Enterococcus faecium strains on internal milieu, antioxidant status and body weight of broiler chickens.

The aim of the present study was to evaluate the functional efficiency of two probiotic strains Lactobacillus fermentum CCM 7158 and Enterococcus faecium M 74 given to the drinking water on internal milieu, antioxidant status and body weight of broiler chickens. The experiment was conducted on hybrid Hybro (n = 180). The feeding period lasted 42 days. Experimental chickens of E1 group received a probiotic preparation in drinking water with concentration of 1 × 10(9) colony forming units (CFU) of L. fermentum CCM 7158 in 1 g of nutrient medium and experimental chickens of E2 group concentration of 2 × 10(9) CFU of E. faecium M 74 in 1 g of nutrient medium. The control group of animals received water without any additives. Triglycerides content in serum mainly with L. fermentum strain against the control group was decreased. Calcium content in both experimental groups and significantly in E. faecium group was increased. Antioxidant status in both probiotic groups was significantly increased. The content of bilirubin in group with E. faecium M 74 was significantly increased. In conclusion, addition of a microbial feed additive (L. fermentum and E. faecium) increased serum calcium and iron level, decreased triglycerides content in blood and slightly increased body weight of broiler chickens.

Comparative analysis of acetic and citric acid on internal milieu of broiler chickens

The aim of the present study was to analyse the effect of two organic acids (acetic and citric acid) inclusion on serum parameters and the level of antioxidant status of broiler chickens. Some organic acidifiers reduce the growth of many intestinal bacteria, reduce intestinal colonisation and reduce infectious processes, decrease inflammatory processes at the intestinal mucosa, increase villus height and function of secretion, digestion and absorption of nutrients. Broiler chickens hybrid Ross 308 (n=180) were divided into 3 groups: one control (C) and two experimental groups (E1, E2). Experimental animals received acetic and citric acid per os in water in single dose 0.25% for 42 days. After 42 days of feeding blood samples were collected (n=10 in each group). Significant decrease of serum triglycerides in citric acid group when compared with the control group was recorded. Acetic acid administration resulted in increased sodium level. Significant increase of albumin content in both experimental groups and increase of bilirubin content in citric group was recorded. Acids administration had no significant effect on other serum and antioxidant parameters. Acetic and citric acid had no harmful influenced on internal milieu of broiler chickens. The research on the field of organic acid will be worthy of further investigation.

Effectiveness of non-antibiotic stimulators in Japanese quail diet: Gender comparison and economical annex

Abstract Economical as well as medicinal and nutritional benefits of Japanese quails are important. The aim of the present study was to investigate the effect of dietary inclusion of probiotics and humic acids on some antioxidant, carcass and egg production parameters of Japanese quails. As Japanese quails are an interesting animal model attracting attention in the last years, a partial aim of this study is also a brief economical annex to the topic. A total of 60 birds (30 males and 30 females, age of 35 days) were involved in the experiment. Animals were fed by standard basal diet and divided to 6 experimental groups as follows: two groups (10 females and 10 males) received addition of probiotics preparation in a single dose of 1 g kg-1 of feed mixture, two groups (10 females and 10 males) received a preparation of humic acids in a single dose of 3 g kg-1 of feed mixture. The groups (10 females and 10 males) fed on basal diet without any additive served as the control group. After 210 days the animals were euthanized and blood samples, carcass organs and abdominal fat were collected. Antioxidant/oxidant parameters (activity of superoxide dismutase SOD, glutathione peroxidase GPx, total antioxidant status TAS, content of bilirubin and reactive oxygen species ROS) from blood samples were measured. The production of eggs was recorded during the experiment. Probiotic and humic acids treatments resulted in significant increase of TAS in females in contrast to males. Content of bilirubin was significantly lower in females fed with addition of humic acids in comparison to the male group. In both treatments (probiotics and humic acids) significantly lower amount of abdominal fat in females in comparison to the controls were recorded. The other investigated parameters of Japanese quails were not influenced by the treatments. The effect of probiotics and humic acids is probably dose dependent and the assessment of an accurate dose of substantives used in poultry feeding plays a crucial role.

ORIGINAL ARTICLE: Effect of Lactobacillus fermentum and Enterococcus faecium strains on internal milieu, antioxidant status and body weight of broiler chickens

The aim of the present study was to evaluate the functional efficiency of two probiotic strains Lactobacillus fermentum CCM 7158 and Enterococcus faecium M 74 given to the drinking water on internal milieu, antioxidant status and body weight of broiler chickens. The experiment was conducted on hybrid Hybro (n = 180). The feeding period lasted 42 days. Experimental chickens of E1 group received a probiotic preparation in drinking water with concentration of 1 × 10(9) colony forming units (CFU) of L. fermentum CCM 7158 in 1 g of nutrient medium and experimental chickens of E2 group concentration of 2 × 10(9) CFU of E. faecium M 74 in 1 g of nutrient medium. The control group of animals received water without any additives. Triglycerides content in serum mainly with L. fermentum strain against the control group was decreased. Calcium content in both experimental groups and significantly in E. faecium group was increased. Antioxidant status in both probiotic groups was significantly increased. The content of bilirubin in group with E. faecium M 74 was significantly increased. In conclusion, addition of a microbial feed additive (L. fermentum and E. faecium) increased serum calcium and iron level, decreased triglycerides content in blood and slightly increased body weight of broiler chickens.

Effect of different levels of green tea (Camellia sinensis) on productive performance, carcass characteristics and organs of broiler chickens

In this work we aimed to determine the effect of different levels of green tea in powder form to feed on productive performance, carcass parameters and organs in broiler chickens. Totally 240 day-old broiler chickens Ross 308 were divided to four dietary groups (n = 60) namely control and three experimental groups with supplementation of green tea to feed mixture in levels 0.5%, 1% and 1.5%. Broiler chickens were feeding with commercial feed mixtures and feed and drinking water were provided ad libittum . The feeding period lasted 42 days. Individual body weight of broiler chickens was determined at 1, 7, 14, 21, 28, 35 and 42 day, feed sonsumption and mortality per group were determined at 42 day of fattening period. Carcass quality and organs weight of broiler chickens were determined at the end of the experiment. The results indicated that supplementation of different levels of green tea statistically significant decreased body weight gain and we recorded lower body weight in 21 days of age compared with control group. However, in second period of fattening, broiler chickens in experimental groups growing faster and in 42 days of age we found statistically no significantly differences among control and experimental groups. Feed consumption did not differ among the dietary groups at 42 days of fattening. Mortality no affected by supplementation of green tea to broiler chickens diets in comparison with control group. From the carcass parameters addition of green tea significantly decreased percentage of abdominal fat between control and 1.5% green tea level, in other parameters (percentage of breast, percentage of drumstick, carcass yield) were different among control and experimental groups not statistically significant. The caecum and small intestine weights was significantly ( p ≤0.05) decreased in chickens fed diets containing 0.5% green tea supplement compared to 1% and 1.5%. For neck, crop, heart, liver, proventriculus, gizzard, pancreas, kidneys, small intestine, caecum and large intestine weights among control and experimental groups we recorded no statistical differences ( p ˃0.05). Normal 0 21 false false false SK X-NONE X-NONE

The effect of feed additives in pheasants fattening: A review

Fattening pheasants for the production of quality meat is a relatively recent development. With its high protein and low fat content, meat of pheasant is a highly nutritious food whose value exceeds that of broiler chickens meat. Despite the fact that the intensive rearing of pheasants has developed relatively quickly in recent years, information on the fattening capabilities and slaughter values of pheasants is often insufficient in the current literature. The length of the fattening period also differs in the literature and reported from 13 to 20 weeks while highest weight gains of pheasants to occur between 4 and 12 weeks of age. Growth of pheasants is also reflected in the weight and dimensions of internal organs, crop, oesophagus, gizzard and intestine grow up to 12 weeks of age, after which they develop their physiological ability. The structure, weight and length of the digestive tract and other internal organs of pheasants depend on housing system, gender and nutrition. Relatively few investigations were conducted to determine the influence of feed additives on fattening performance of pheasants. One of the possibilities is the use of humic acids in nutrition pheasant. Humic acids are organic compounds naturally present in soil and they positively affected growth ability, feed conversion and reducing mortality.

Účinok humínových kyselín a cesnaku (Allium sativum L.) na produkčné parametre a jatočnú charakteristiku brojlerových kurčiat

In this study the effect of humic acids and humic acids and garlic powder (Allium sativum L.) combination on performance parameters and carcass characteristic of broiler chickens were studied. A total of 120 Ross 308 broiler chickens were divided into 3 treatments (n=40). The control group of chickens was fed complete feed mixtures without any additives. Treatment T1 was fed complete feed mixtures containing 1% of humic acid. Treatment T2 was fed complete feed mixtures containing 1.8% of humic acid and 0.2% of garlic powder (Allium sativum L.). At the end of the experiment was average body weight (values in the order of the groups: 1796.4±188.1; 1999.1±355.8 and 1958.6±201.2 g±SD) significantly higher (P≤0.05) in both treatments groups compared to control group. In T1 was weight of gizzard (values in the order of the groups: 34.9±5.2; 43.1±9.4 and 38.9±7.0 g±SD) significantly higher (P≤0.05) compared to control group. Carcass weight, weight of heart weight of liver and carcass yield of treatments groups was higher, but differences in these indicators were not statistically significant (P≥0.05). The results of this experiment indicate that humic acids may by an alternative replacement of feed antibiotics.

ORIGINAL ARTICLE: Effect of Lactobacillus fermentum and Enterococcus faecium strains on internal milieu, antioxidant status and body weight of broiler chickens: Probiotics and internal milieu of chickens

The aim of the present study was to evaluate the functional efficiency of two probiotic strains Lactobacillus fermentum CCM 7158 and Enterococcus faecium M 74 given to the drinking water on internal milieu, antioxidant status and body weight of broiler chickens. The experiment was conducted on hybrid Hybro (n = 180). The feeding period lasted 42 days. Experimental chickens of E1 group received a probiotic preparation in drinking water with concentration of 1 × 10(9) colony forming units (CFU) of L. fermentum CCM 7158 in 1 g of nutrient medium and experimental chickens of E2 group concentration of 2 × 10(9) CFU of E. faecium M 74 in 1 g of nutrient medium. The control group of animals received water without any additives. Triglycerides content in serum mainly with L. fermentum strain against the control group was decreased. Calcium content in both experimental groups and significantly in E. faecium group was increased. Antioxidant status in both probiotic groups was significantly increased. The content of bilirubin in group with E. faecium M 74 was significantly increased. In conclusion, addition of a microbial feed additive (L. fermentum and E. faecium) increased serum calcium and iron level, decreased triglycerides content in blood and slightly increased body weight of broiler chickens.