Henrieta Arpášová

Nickel induced alteration of hen body weight, egg production and egg quality after an experimental peroral administration.

In this study the effects of nickel (NiCl2) administered in drinking water (0.02; 0.2 and 2.0 mg NiCl2/L for 28 days) on laying hen body weight, egg production and egg quality is reported. Growth parameters during the experiment were significantly decreased mainly in the group with the highest nickel concentration. In total egg production dose–dependent decrease in all experimental groups was found. Egg weight was mainly affected in the group with the highest nickel concentration. Specific egg weight was not altered. Albumen weight and albumen content was significantly decreased in groups with the highest nickel concentration in comparison with the control group. Egg yolk analysis detected significantly decreased yolk weight in the group with the highest nickel experimental level. In yolk color a significant difference was detected between the group receiving 0.02 and 0.2 mg NiCl2/mL. Eggshell compactness was increased in all experimental groups what could be induced by altered mineralization of eggshell. Results of this study clearly report a negative effect of nickel as an environmental pollutant on laying hen body weight, egg production as well as egg quality.

Nickel-induced blood biochemistry alterations in hens after an experimental peroral administration.

The purpose of this study was to determine certain blood biochemical parameters in hens of Isa Brown breed (n = 20) after nickel administration. Animals were divided into four groups (K, P1, P2, P3). Experimental hens (n = 5; in each group) received nickel (NiCl2) as peroral administration in drinking water in various doses (P1 – 0.02 g NiCl2/L; P2 – 0.2 g NiCl2/L; P3 – 2.0 g NiCl2/L of drinking water) for 28 days. The last group – K (n = 5) was the control, receiving no nickel. Biochemical parameters of mineral profile (calcium; phosphorus; magnesium; sodium; potassium) and of energy and enzymatic profile [(glucose; total cholesterol; total proteins; triglycerides; alanine aminotransferase (ALT) aspartate aminotransferase (AST) gamma glutamyl transferase (GGT) and glutamatdehydrogenase (GLDH)] were analyzed in blood serum on Day 0, 7, 14, 21 and 28 of the experiment. Average levels of mineral metabolism parameters were relatively stable apart from calcium. The evaluation registered a significant decrease in calcium during the experiment mainly in the group with highest nickel concentration in drinking water. No significant differences were detected between groups in energy and enzymatic profile apart from the concentrations of ALT on Day 7. In conclusion, there were significant associations between nickel levels and calcium and ALT in blood serum of the hens. No significant differences were detected in other biochemical parameters of mineral profile (P, Mg, Na, K) and energy and enzymatic profile (glucose, total cholesterol, total proteins, triglycerides, AST, GGT and GLDH) after nickel administration. Our results may contribute to an evaluation of reference levels of analyzed parameters, to monitor the health and nutritional status of hens. In this study also the negative effect of nickel mainly on calcium metabolism was detected.

In vitro and In vivo antimicrobial activity of propolis on the microbiota from gastrointestinal tract of chickens

The aim of this study was to examine the effect of propolis extracts on the microbial colonization of chicken gastrointestinal tract in vivo. The propolis was administered to both feed mixtures in various amounts except of the control group. The addition of 150 mg propolis to 1 kg of feed was included in the first experimental group, the addition of 450 mg.kg−1 in the second experimental group, the addition of 600 mg.kg−1 the third experimental group and 800 mg kg−1 in the fourth one. The highest count of faecal enterococci was found in the third group (8.6 cfu.g−1) where 600 mg of propolis to 1 kg was added to the feed mixture. The highest count of lactobacilli was detected in the fourth experimental group (8.83 cfu.g−1) where was 800 mg of propolis added to 1 kg of feed mixture and number of Enterobacteriaceae genera count was found in control group (8.73 cfu.g−1). With RTQ PCR detected species from the genus Enterococcus were: E. avium, E. casseliflavus, E cecorum, E. faecalis, E. faecium, E. gallinarum, E. hirae and E. malodoratus and from genus Lactobacillus were: Lactobacillus crispatus, L. acidophilus and L. salivarius. With MALDI TOF MS Biotyper from Enterobacteriaceae genera were identified Citrobacter braakii, Raoultella ornithinolytica, Serratia fonticola, Escherichia coli and Klebsiella oxytoca. Antimicrobial activities In vitro of six species of bacteria isolated from gastrointestinal tract of chickens were also tested. The best antimicrobial effect of Citrobacter braakii on ethanolic propolis extract in all concentrations were found.

The effects of bee pollen extracts on the broiler chicken's gastrointestinal microflora

The aim of this study was to investigate the effects of bee pollen ethanolic extracts on the in vivo gastrointestinal tract microflora colonization of broiler chickens. A completely randomized experiment based on six treatments (different concentrations of bee pollen - 0, 5, 15, 25, 35 and 45 g kg(-1) diet) was used during 7 weeks. The highest count of faecal Enterococci was found in the experimental group with the addition of 15 g of pollen (8.85 ± 0.87 log CFU g(-1)) per 1 kg of feed mixture. The highest count of Lactobacilli was detected in the experimental group with 35 g of pollen per 1 kg of feed mixture and the highest number of the Enterobacteriaceae genera count was found in the control group (8.43 ± 0.15 log CFU g(-1)). Moreover, the MALDI TOF MS Biotyper identified the following genera: Escherichia coli, Proteus mirabilis, Klebsiella oxytoca, as well as Lactobacillus acidophilus, L. crispatus, L. fermentum and L. salivarius from the Lactobacilli group and Enterococcus avium, E. casseliflavus, E. cecorum, E. faecalis, E. faecium, E. gallinarum, E. hirae and E. malodoratus from the Enterococci group. Additionally, the in vitro antimicrobial activities of pollen against five bacteria species isolated from gastrointestinal tracts of chickens were tested. The best antimicrobial effect of the pollen extract was detected against K. oxytoca.

The effect of a phytogenic additive on nutritional composition of turkey meat

The aim of the study was to analyze the effect of a blend of phytogenic additive on nutritional and mineral composition of breast, thigh muscles and liver in fattening turkeys. A total 300 female turkeys were monitored in the trial. 1-day old broad-breasted white turkeys hybrid XL were randomly divided into two groups (150 pcs per each). Turkeys in control group were fed by standard diet for fattening and in experimental group from the 1st to the 12th week by complete feed mixture with supplementation of a blend of essential oils from origanum, anise and citrus fruits as well as a prebiotic rich fructooligosaccharides in dosage 1 kg per 1000 kg of feed mixture. Turkeys were housed in group on deep litter. Experiment lasted 18 weeks. Samples of breast and thigh muscles, and liver for nutritional analysis were collected during turkey’s dissection (10 samples per each group). After the determination of nutrients, there were found significant (P 0.05) content of DM and fat was recorded in liver. After analysis the macro and microelements, significant (P 0.05) concentrate only in content of manganese in experimental group. Occurrence of manganese was not observed in both muscles.

EFFECT OF SELECTED FEED ADDITIVES ON INTERNAL QUALITY PARAMETERS OF TABLE EGGS

The aim of our experiment was to evaluate under experimental conditions the influence of probiotic preparation based on lactobacillus, oregano essential oil, sumac ( Rhus coriaria ), propolis and pollen on egg quality parameters of laying hens hybrid Lohman Brown. For housing hens (n ​​ = 60) three storey enriched battery cage was used in which hens were divided in groups (n = 10). Total floor space given to one animal was 943.2 cm 2 . In the control group of hens complete feed mixtures without any additions were fed. In the first experimental group complete feed mixture was enriched with probiotic lactobacilli based preparation in a dose of 0.5 g.kg -1 . In the second experimental group oregano essential oil was added to the feed mixture at a dose of 0.5 ml.kg -1 . The third experimental group was enriched with 1 % concentration of sumac ( Rhus coriaria ). The fourth experimental group was enriched with 0.2 g of propolis extract per 1 kg of feed mixture and the fifth experimental group was supplemented by pollen extract of the same dose. All groups were fed ad libitum . Eggs quality indicators observed were egg weight (g), yolk percentage (%), yolk index, white percentage (%), whites index and Haugh units of whites (HJ). The results show that addition of probiotics positively, although not significantly, influenced the average egg weight. The addition of oregano oil and sumac insignificantly decreased egg weight (P>0.05), the values ​​ of the other eggs quality indicators were comparable with the control group. Supplementation of feed mixture with propolis as well as phytobiotics insignificantly decreased egg weight, but its addition improved the internal quality parameters as the yolks and whites index, whose average values ​​ were in this group, although not statistically significantly higher (P>0.05). doi:10.5219/235

CHEMICAL COMPOSITION OF MUSCLE AFTER POLLEN APPLICATION IN NUTRITION OF BROILER CHICKENS

Principle purpose of this study was to monitor the chemical composition of breast and thigh muscular part of chickens of hybrid combination Ross 308 and to verify the differences due to use of pollen extract in feed mixture at a dose of 400 and 800 mg.kg -1 during the feeding of 42 days. We did not find significant differences (P≥0.05) in followed chemical compounds of thigh muscle with the skin and subcutaneous fat between the groups with and without application of pollen extract: in contents of water (68.49-70.12 g.100g -1 ), protein (18.82-18.98 g.100g -1 ), fat (9.96-11.53 g.100g -1 ) and in energy value (692.20-752.36 kJ.100g -1 ). Significant differences (P≤0.05) were found only in protein content of breast muscle between the control group (23.96 g.100g -1 ) and experimental group I (23.28 g.100g -1 ). Values of water content (73.97-74.32 g.100g -1 ), fat content (1.07-1.40 g.100g -1 ) and energy (441.65-446.64 kJ.100g -1 ) in breast muscle between the evaluated groups were balanced (P≥0.05). The results show that pollen extract at the concentration used in feed mixture did not effect basic chemical composition of the most valuable parts of the chicken Ross 308 carcass and we can apply it in their nutrition. Application at a dose of 800 mg.kg -1 seems to be more positive.

EFFECT OF DIETARY SODIUM SELENITE AND SE-ENRICHED YEAST ON EGG-SHELL QUALITATIVE PARAMETERS OF LAYING HENS EGGS

The experiment was designed to investigate the effects of feed supplementation with selenite or selenized yeast on eggs quality of laying hens. Hens of laying breed Isabrown were randomly divided at the day of hatching into 4 groups (n=12) and fed ad libitum for 9 months on diets which differed only in amounts or forms of selenium supplemented. Hens were fed from 1st day by standard feed mixture. Control group get only native dose of selenium (0.1 mg/kg) naturally presented in feed mixtures. First experimental group get selenium addition 0.4 mg/kg in a form of sodium selenite, second one the same dose of 0.4 mg/kg but in organic form of Se-yeast. The diet for the fourth group was supplemented with Se-yeast at Se dose 0.9 mg/kg DM. The both doses of organic selenium had significantly (P 0.05) by the supplementation of Se into the feed mixture for laying hens.

INFLUENCE OF PLANT ESSENTIAL OILS ON SELECTED PARAMETERS OF THE PERFORMANCE OF LAYING HENS

The experiment was designed to investigate the effects of feed supplementation with essential oils on egg weight and body mass of laying hens. Hens of the laying breed Isa Brown were randomly divided at the day of hatching into 3 groups (n=26) and fed for 45 weeks on diets which differed in kind of essential oil supplemented. Hens were fed from day 1 by the standard feed mixture. Laying hens accepted fodder ad libitum. In the control group hens took feed mixture without additions, in the first experimental group the feed mixture was supplemented with 0.25 ml/kg thyme essential oil and in the second one hens got hyssop essential oil in the same dose of 0.25 ml/kg. The housing system satisfied enriched cage requirements specified by the Directive 1999/74 EC. The useful area provided for one laying hen presented 943.2 cm2. The equipment of cage consisted of roosts, place for rooting in ashes – synthetic grass, nest and equipment for shortening of clutches. The results showed that the average body weight for a rearing period was in order groups: 736.15±523.49; 747.20±541.6 and 721.95±522.57 (g±SD). Differences between groups were not significant (P>0.05). The average body weight during the laying period was 1763.85±171.46; 1786.08±192.09 and 1729.73±129.12 g for control, thyme oil and hyssop oil supplementation respectively. During the laying period there were significant differences in body weight between control and experimental group with hyssop essential oil supplementation (P 0.05) in egg weight (58.36±4.91; 58.82±4.95 and 58.26±5.33 g respectively).

Chemical composition of chicken meat after application of humic acid and probiotic Lactobacillus fermentum

The aim of the present study was analysed and evaluated chemical parameters of chicken breast and thigh muscles after addition of humic acids and probiotic into diet for broiler chicken. A total of 200 pcs Ross 308 broiler chickens were divided into 4 groups (n=50). The control group of chickens was fed with complete feed mixtures without any additives. Chickens in experiment groups were fed a diet containing: P1 (1% of humic acid), P2 (1% of humic acid and probiotic supplement Lactobacillus fermentum ) and P3 were fed with complete feed mixture containing combination of starter feed mixture (1. - 21. day) with coccidiostaticum Diclazuril and growth feed mixture (21. - 35. day) containing Salinomycinum sodium. Besides, the groups were kept under the same conditions. Fattening period lasted for 42 days. Chicken meat was analyzed for content of water, crude protein, fat and cholesterol. Based on the results, we can state that the application of humic acids or the combination of Humac Natur with probiotic did not affect the chemical composition of the breast muscle. In the breast muscle, the protein content in the experimental group P3 with the coccidiostat (22.98 g.100 g -1 ) was reduced ( p ≤0.05) compared to control group (23.42 g.100 g -1 ). In the case of thigh muscle was significantly higher content of fat and cholesterol ( p ≤0.05) in chickens feeding with addition of Humac Natur (fat - 9.08 g.100g -1 ; cholesterol - 0.86 mg.100g -1 ) and similar results were recorded in experimental group with combination of Humac Natur and probiotic (fat - 9.15 g.100g -1 ; cholesterol - 0.86 mg.100g -1 ) compared to control group (fat - 7.15 g.100g -1 ; cholesterol - 0.70 mg.100g -1 ). From a general point of view, we can recommend the application of Humac Natur, respectively combination Humac Natur with probiotics in feeding of broiler chickens Ross 308. 14.00 Normal 0 21 false false false EN-GB X-NONE X-NONE