D. J. Fagerberg

Enrichment and plating methodology for Salmonella detection in food. A Review

Much research concerned with enrichment and plating methodology to detect salmonella contamination in foods has been reported by many scientists. This review brings reported findings of major proponents together into one text for greater understanding and appreciation of the complexity of the problem. Discussed in this review are reported applications and mechanisms of 11 enrichment media and eight plating media, incubation temperatures and times, and serotype specificity involving enrichment and plating media. Also, enrichment emulsifying agents, agitation during incubation, sample type, and level and proportion of salmonellae and competitors as related to salmonella enrichment are discussed. Other factors related to salmonella recovery, such as multiple media methods, preparation methods, storage of media, and media brand are included in this review, as well as a discussion of the methodology dilemma and some general recommendations for future direction.

Recovery of 61 Salmonella Serotypes from Artificially Contaminated Turkey Skin

Selenite brilliant-green sulfa enrichment broth containing Tween 80 was streaked to brilliant-green sulfa plates for use in comparing recoverability of 61 different salmonella serotypes from turkey tails artifically contaminated with very low levels of each serotype. Recoverability was 100% with 43 of the serotypes, 80% with eight, 60% with four, and 40% or less with six.

Importance of Brands of Dehydrated Culture Medium

Selenite Brilliant Green Sulfa broths, one prepared from basic ingredients according to formula and brands A and B available in dehydrated form, were compared for effectiveness of recovering salmonellae. Distinctly different percent recoveries of salmonellae were achieved among the three types of media.