D. J. Hatch

Gross nitrogen fluxes in soil : theory, measurement and application of 15N pool dilution techniques

Abstract Isotopic pool dilution using 15 N is proving to be a valuable tool for increasing our understanding of gross N cycling processes and our ability to both model these processes and link them to microbial function. However, not all applications are appropriate. Many of the questions asked by agronomists and soil scientists can often be addressed by simpler experiments in which measurements of the main parameters of inorganic and total N content of soil and plant components would suffice. In addition, the theory, assumptions and techniques associated with the calculation of gross N fluxes can lead to large errors if not applied correctly. Some preliminary assessment of the principle N transformation processes to be studied, followed by an optimisation of the experimental conditions are needed for the effective application of 15 N pool dilution. When applied correctly under carefully controlled laboratory incubations, the technique has been used successfully to quantify gross N fluxes and to understand the fundamental processes that regulate individual microbial N pathways. This has improved our understanding of how C and N cycles are linked, and thus has led us to question the most appropriate structure of C and N cycling models. Field based 15 N pool dilution studies have been used successfully to study the climatic influence on the soil N cycle and also to quantify the impact of external inputs. Further field-based studies are required to aid model development and evaluation. Linking soil microbial/molecular ecology with process-based studies of microbial nutrient cycling presents a new and exciting field of research that will benefit from the further application of isotopic pool dilution techniques for N and other nutrients.

Laboratory study of the effects of two nitrification inhibitors on greenhouse gas emissions from a slurry-treated arable soil: impact of diurnal temperature cycle

An automated laboratory soil incubation system enabled the effects on gaseous emissions from a soil to be quantified accurately, when amended with slurry plus a nitrification inhibitor: dicyandiamide (DCD), or 3,4-dimethylpyrazole phosphate (DMPP). Nitrification inhibitors applied with slurry under simulated Portuguese conditions were very efficient in reducing N2O emission, and did not increase CH4 emissions significantly, when the soil was predominantly aerobic. The inhibitors were also indirectly effective in reducing N2O emissions due to denitrification during a subsequent anaerobic phase. All gaseous emissions followed strong diurnal patterns that were positively correlated with soil temperature and obeyed a Q10=2 relationship. The widespread use of DCD and DMPP inhibitors with slurry applied to Portuguese soils could have the potential to reduce N2O emissions from this source by ten- to 20-fold.

Gross and net rates of nitrogen mineralisation in soil amended with composted olive mill pomace.

Olive mill pomace is the major waste product in the olive oil industry and composting these by-products for the purpose of recycling nutrients and organic matter is a sound environmental strategy. Yet little is known about the quantity and timing of nitrogen (N) release from composted olive mill pomace. This paper assesses both gross (using the (15)N dilution technique) and net (aerobic incubation) nitrogen (N) mineralisation and N(2)O emissions of soil amended with seven commercially available composts of olive mill pomace (COMP). All are currently produced in Andalusia and differ in the proportions of raw materials co-composted with the pomace. The absence of significant differences in net N or gross mineralisation and nitrification in COMP-amended soil compared with a control, except for COMP combined with poultry manure, highlighted the recalcitrant nature of the COMP-N. Applications of COMP are hence unlikely to supply available N in available forms, at least in the short-term. Furthermore, N(2)O emissions from COMP-amended soil were negligible and, therefore, applications in the field should not result in increased N loss through denitrification.

Nitrogen Management for Profitable Farming with Minimal Environmental Impact: The Challenge for Mixed Farms in the Cotswold Hills, England

A detailed nitrogen (N) budget was constructed for a mixed farm in the Cotswold Hills, England, situated on thin, well drained soils prone to leaching. The study covered all stages of the farms seven-year rotation and included the removal of the dairy herd. All inputs and outputs of N were measured or estimated and a balanced budget achieved, but only by including relatively expensive measurements of soluble organic nitrogen (SON) leached. Leaching was the main loss process. Given the nature of the soil and the influence of the weather, it would be difficult to reduce losses without drastic reductions in fertiliser inputs or stocking rates. Nitrogen use efficiency averaged 46%. The mean N surplus declined from 141 kg N ha−1 to 117 kg N ha−1 with the removal of the dairy herd. However, the farm to which the herd moved had an N surplus of 392 kg N ha−1. Simple farm gate N budgets were constructed for neighbouring Cotswold farms to encourage farmers to consider ways to improve N use. Implications for policy to reduce losses of N while maintaining farm profitability are discussed.

Carbon mineralization and distribution of nutrients within different particle-size fractions of commercially produced olive mill pomace

Composting is a realistic option for disposal of olive mill pomace (OMP) by making it suitable as a soil amendment for organic farming. The chemical and physical characteristics and contribution of particle-size fractions to total nutrients and carbon mineralization of seven commercial composts of OMP (COMP) were investigated. Higher proportions of manure, co-composted with OMP, reduced the organic matter (OM), total carbon and C:N ratio of the product, but increased the content of nutrients and fine particles. The fine particles had higher nutrient contents, but less OM and carbon and, unlike larger particles, did not exhibit any phytotoxicity. Less than 1.5% of added carbon was mineralized in whole compost, but a lower rate was found with larger particles. Separation of COMP by particle size fractionation and application as a soil conditioner is recommended for better optimization of COMP with the <1mm fraction providing the higher quality compost.

Potential mineralization and nitrification in volcanic grassland soils in Chile

Abstract A proportion of the nitrogen (N) applied to grasslands as organic or inorganic fertilizers can be lost to water courses as nitrate and to the atmosphere as nitrous and nitric oxides. Volcanic soils from Chile are not generally prone to leaching, possibly due to net immobilization of nitrate and/or ammonium, and/or due to inhibition of nitrification by either chemical or physical processes. In laboratory studies we found large mineralization potentials in soils from three different Chilean soils after 17 weeks of incubation, totalling 215 and 254 mg kg−1 dry soil for two Andisols and 127 mg kg−1 dry soil in an Ultisol. Nitrification occurred after a short period, and was lowest in the Ultisol. In addition, microbial analysis showed nitrifiers to be present in all three soils. Adsorption of ammonium was two-fold stronger than for nitrate, ranging from 29 to 180 kg N ha−1. The highest potential for N adsorption in the 0–60 cm soil profile was with the Ultisol (398 kg N ha−1), but was similar in both Andisols (193 and 172 kg N ha−1, respectively). The combination of ammonium retention together with delayed nitrification could account for the low leaching rates in these soils.

The Compost of Olive Mill Pomace: From a Waste to a Resource - Environmental Benefits of Its Application in Olive Oil Groves

© 2012 Gomez-Munoz et al., licensee InTech. This is an open access chapter distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Compost of Olive Mill Pomace: From a Waste to a Resource – Environmental Benefits of Its Application in Olive Oil Groves

Evidence for the production of NO and N2O in two contrasting subsoils following the addition of synthetic cattle urine

Nitrogenous materials can be transferred out of the topsoil, either vertically to a greater depth, or in lateral pathways to surface waters, and they may also become transformed, with the potential of generating environmentally active agents. We measured the production of NO and N(2)O in two contrasting subsoils (70 to 90 cm): one poorly drained and the other freely drained and compared this with the topsoil (0 to 20 cm) of the corresponding soils. The soils were incubated aerobically in jars with subtreatments of either synthetic cattle urine or deionised water and sampled at intervals up to 34 days. (15)N-NO(3)(-) was used to determine the processes responsible for NO and N(2)O production. The headspace was analysed for the concentrations of N(2)O, NO and CO(2) and (15)N enrichment of N(2)O. The soil samples were extracted and analysed for NO(2)(-), NO(3)(-) and NH(4)(+), and the (15)N enrichment of the extracts was measured after conversion into N(2)O and N(2). The study demonstrated the potential for NO, N(2)O and NO(2)(-) to be generated from subsoils in laboratory incubations. Differences in these N dynamics occurred due to subsoil drainage class. In the freely drained subsoil the rates of NO and NO(2)(-) production were higher than those observed for the corresponding topsoil, with mean maximum production rates of 3.5 microg NO(2)(-)-N g(-1) dry soil on day 16 and 0.12 microg NO-N g(-1) dry soil on day 31. The calculated total losses of N(2)O-N as percentages of the applied synthetic urine N were 0.37% (freely drained subsoil), 0.24% (poorly drained subsoil), 0.43% (freely drained topsoil) and 2.09% (poorly drained topsoil). The calculated total losses of NO-N as percentages of the applied synthetic urine N were 1.53% (freely drained subsoil), 0.02% (poorly drained subsoil), 0.25% (freely drained topsoil) and 0.08% (poorly drained topsoil).

High rates of nitrogen cycling in volcanic soils from Chilean grasslands

There are over one million hectares of pasture in Chile, and 80% and 50% of the countrys milk and meat comes from 72% of this area, situated in the lake region of southern Chile. The soils are volcanic and a major characteristic is that they have very high organic matter (OM) contents with the potential to support plant growth with only moderate levels of added nitrogen (N). To understand better the potential fertility of these soils in order to maximise production and minimise losses of N, we undertook studies using the stable isotope of N ((15)N) to resolve the rates of the main internal N cycling processes in three soils representing the two main volcanic soil types: Osorno and Chiloé (Andisol) and Cudico (Ultisol). We also assessed the longer-term potential of these soils to sustain N release using anaerobic incubation. Gross rates (µg N g(-1) day(-1)) of mineralisation were 27.9, 27.1 and 15.5 and rates of immobilisation were 5.9, 12.0 and 6.3 for Osorno, Chiloé and Cudico, respectively, implying high rates of net mineralisation in these soils. This was confirmed by anaerobic incubation which gave potential seasonal net mineralisation indices of 1225, 1059 and 450 kg N ha(-1) in the top 10 cm soil layers of the three soils. However, plant production may still benefit from added N, as the release of N from organic sources may not be closely synchronised with crop demand. The low rates of nitrification that we found with these acidic soils suggest that the more mobile N (viz. nitrate-N) would be in limited supply and plants would have to compete for the less mobile ammonium-N with the soil microbial biomass. Nitrogen was mineralised in appreciable amounts even down to 60 cm depth, so that leaching could become significant, particularly if the soils were limed, which could enhance nitrification and N mobility through the soil profile.

Soil mineral N retention and N2O emissions following combined application of 15N-labelled fertiliser and weed residues†

RATIONALE The combination of plant residues with inorganic fertiliser-N provides the potential to increase N-use efficiency in agricultural fruit production systems, such as olive orchards. The development of weeds in the inter-canopy area of olive orchards is encouraged as a novel strategy to reduce soil erosion. However, little is known about soil N retention or N(2) O production following the combined application of inorganic-N with the mulched weed residues. METHODS Emissions of (15) N-N(2) O and soil mineral (15) N retention were measured following combined applications of (15) N-labelled fertiliser and a range of olive crop weed residues to a silty loam soil under controlled conditions. These plant residues differed in their C:N ratios, lignin and polyphenol contents. RESULTS The magnitude of soil (15) N-NO(3) (-) retention from combining plant residues and fertiliser-N was highly dependent on potential N mineralisation (r = -0.96) and the (lignin + polyphenol)-to-N ratio (r = 0.98) of the residues. Fertiliser-N-derived retention was zero for a legume-based mulch but up to 80% in the treatment containing plant residues with a high (lignin + polyphenol)-to-N ratio. N(2) O emissions increased after the addition of residues, and increased further (up to 128%) following the combined application of inorganic fertiliser and residues. Fertiliser-derived (15) N-N(2) O was <1.4% of the total (14+15) N-N(2) O emission and <0.01% of the applied (15) N-NO(3) (-) . Enhanced N(2) O emissions following the application of residues and the fertiliser-N values were positively correlated with the C:N ratio of the residue. Thus, combining organic- and inorganic-N immobilised a significant proportion of the inorganic N with little increase in N(2) O, especially in low C:N ratio residues. CONCLUSIONS The results demonstrate that whilst there is potential for N(2) O emissions to be controlled by combining weed residues and inorganic fertilisers, this is not easy to achieve as the magnitude and direction of interactions vary between different species due to their varying substrate qualities.