D. P. Jacomassi

Investigation of the photodynamic effects of curcumin against Candida albicans.

This study describes the association of curcumin with light emitting diode (LED) for the inactivation of Candida albicans. Suspensions of Candida were treated with nine curcumin concentrations and exposed to LED at different fluences. The protocol that showed the best outcomes for Candida inactivation was selected to evaluate the effect of the preirradiation time (PIT) on photodynamic therapy (PDT) effectiveness, the uptake of curcumin by C. albicans cells and the possible involvement of singlet oxygen in the photodynamic action. Curcumin‐mediated PDT was also assessed against biofilms. In addition to the microbiological experiments, similar protocols were tested on a macrophage cell line and the effect was evaluated by Methyltetrazolium assay (MTT) and SEM analysis. The optical properties of curcumin were investigated as a function of illumination fluence. When compared with the control group, a statistically significant reduction in C. albicans viability was observed after PDT (P < 0.05), for both planktonic and biofilm cultures. Photodynamic effect was greatly increased with the presence of curcumin in the surrounding media and the PIT of 20 min improved PDT effectiveness against biofilms. Although PDT was phototoxic to macrophages, the therapy was more effective in inactivating the yeast cell than the defense cell. The spectral changes showed a high photobleaching rate of curcumin.

The filler content of the dental composite resins and their influence on different properties.

The purpose of this study was to compare the inorganic content and morphology of one nanofilled and one nanohybrid composite with one universal microhybrid composite. The Vickers hardness, degree of conversion and scanning electron microscope of the materials light‐cured using LED unit were also investigated. One nanofilled (Filtek™ Supreme XT), one nanohybrid (TPH®3) and one universal microhybrid (Filtek™ Z‐250) composite resins at color A2 were used in this study. The samples were made in a metallic mould (4 mm in diameter and 2 mm in thickness). Their filler weight content was measured by thermogravimetric analysis (TG). The morphology of the filler particles was determined using scanning electron microscope equipped with a field emission gun (SEM‐FEG). Vickers hardness and degree of conversion using FT‐IR spectroscopy were measured. Filtek™ Z‐250 (microhybrid) composite resin shows higher degree of conversion and hardness than those of Filtek™ Supreme XT (nanofilled) and TPH®3 (nanohybrid) composites, respectively. The TPH3® (nanohybrid) composite exhibits by far the lowest mechanical property. Nanofilled composite resins show mechanical properties at least as good as those of universal hybrids and could thus be used for the same clinical indications as well as for anterior restorations due to their high aesthetic properties. Microsc. Res. Tech. 75:758–765, 2012.

Effect of power densities and irradiation times on the degree of conversion and temperature increase of a microhybrid dental composite resin

The different parameters used for the photoactivation process provide changes in the degree of conversion (DC%) and temperature rise (TR) of the composite resins. Thus, the purpose of this study was to evaluate the DC (%) and TR of the microhybrid composite resin photoactivated by a new generation LED. For the KBr pellet technique, the composite resin was placed into a metallic mould (1-mm thickness and 4-mm diameter) and photoactivated as follows: continuous LED LCU with different power density values (50–1000 mW/cm2). The measurements for the DC (%) were made in a FTIR Spectrometer Bomen (model MB-102, Quebec-Canada). The spectroscopy (FTIR) spectra for both uncured and cured samples were analyzed using an accessory for the diffuse reflectance. The measurements were recorded in the absorbance operating under the following conditions: 32 scans, 4-cm−1 resolution, and a 300 to 4000-cm−1 wavelength. The percentage of unreacted carbon-carbon double bonds (% C=C) was determined from the ratio of the absorbance intensities of aliphatic C=C (peak at 1638 cm−1) against an internal standard before and after the curing of the specimen: aromatic C-C (peak at 1608 cm−1). For the TR, the samples were made in a metallic mould (2-mm thickness and 4-mm diameter) and photoactivated during 5, 10, and 20 s. The thermocouple was attached to the multimeter to allow the temperature readings. The DC (%) and TR were calculated by the standard technique and submitted to ANOVA and Tukey’s test (p < 0.05). The degree of conversion values varied from 35.0 (±1.3) to 45.0 (±2.4) for 5 s, 45.0 (±1.3) to 55.0 (±2.4) for 10 s, and 47.0 (±1.3) to 52.0 (±2.4) for 20 s. For the TR, the values ranged from 0.3 (±0.01) to 5.4 (±0.11)°C for 5 s, from 0.5 (±0.02) to 9.3 (±0.28)°C for 10 s, and from 1.0 (±0.06) to 15.0 (±0.95)°C for 20 s. The power densities and irradiation times showed a significant effect on the degree of conversion and temperature rise.

Changes in the temperature of a dental light-cured composite resin by different light-curing units

The purpose of this study was to evaluate the temperature increase during the polymerization process through the use of three different light-curing units with different irradiation times. One argon laser (Innova, Coherent), one halogen (Optilight 501, Demetron), and one blue LED (LEC 1000, MM Optics) LCU with 500 mW/cm2 during 5, 10, 20, 30, 40, 50, and 60 s of irradiation times were used in this study. The composite resin used was a microhybrid Filtek Z-250 (3M/ESPE) at color A2. The samples were made in a metallic mold 2 mm in thickness and 4 mm in diameter and previously light-cured during 40 s. A thermocouple (Model 120–202 EAJ, Fenwal Electronic, Milford, MA, USA) was introduced in the composite resin to measure the temperature increase during the curing process. The highest temperature increase was recorded with a Curing Light 2500 halogen LCU (5 and 31°C after 5 and 60 s, respectively), while the lowest temperature increase was recorded for the Innova LCU based on an argon laser (2 and 11°C after 5 and 60 s, respectively). The temperature recorded for LCU based on a blue LED was 3 and 22°C after 5 and 60 s, respectively. There was a quantifiable amount of heat generated during the visible light curing of a composite resin. The amount of heat generated was influenced by the characteristics of the light-curing units used and the irradiation times.

Degree of conversion and temperature increase of a composite resin light cured with an argon laser and blue LED

Different light sources and power densities used on the photoactivation process may provide changes in the degree of conversion (DC%) and temperature (T) of the composite resins. Thus, the purpose of this study was to evaluate the DC (%) and T (°C) of the microhybrid composite resin (Filtek™ Z-250, 3M/ESPE) photoactivated with one argon laser and one LED (light-emitting diode) with different power densities. For the KBr pellet technique, the composite resin was placed into a metallic mould (2-mm thickness, 4-mm diameter) and photoactivated as follows: a continuous argon laser (CW) and LED LCUs with power density values of 100, 400, 700, and 1000 mW/cm2 for 20 s. The measurements for DC (%) were made in a FTIR spectrometer Bomen (model MB 102, Quebec, Canada). Spectroscopy (FTIR) spectra for both uncured and cured samples were analyzed using an accessory of the reflectance diffusion. The measurements were recorded in absorbance operating under the following conditions: 32 scans, 4 cm−1 resolution, 300 to 4000-cm−1 wavelength. The percentage of unreacted carbon double bonds (% C=C) was determined from the ratio of absorbance intensities of aliphatic C=C (peak at 1638 cm−1) against an internal standard before and after the curing of the specimen: aromatic C-C (peak at 1608 cm−1). For T (°C), the samples were created in a metallic mould (2-mm thickness, 4-mm diameter) and photoactivated for 20 s. The thermocouple was attached to the multimeter allowing temperature readings. The DC (%) and T (°C) were submitted to ANOVA and Tukey’s test (p < 0.05). The degree of conversion values varied from 35.0 to 50.0% (100 to 1000 mW/cm2) for an argon laser and from 41.0 to 49% (100 to 1000 mW/cm2) for an LED. The temperature change values varied from 1.1 to 13.1 °C (100 to 1000 mW/cm2) for an argon laser and from 1.9 to 15.0 °C (100 to 1000 mW/cm2) for an LED. The power densities showed a significant effect on the degree of conversion and changes the temperature for both lightcuring units.

Measurement of shrinkage of composite resin by laser speckle contrast analysis

The aim of this study was to evaluate the shrinkage of a microhybrid dental composite resin photo-activated by one LED with different power densities by means of speckle technique. The dental composite resin Filtek™ Z-250 (3M/ESPE) at color A2 was used for the samples preparation. Uncured composite was packed in a metallic mold and irradiated during 20 s from 100 to 1000 mW cm−2. For the photo-activation of the samples, it was used a LED prototype (Light Emission Diode) with wavelength centered at 470 nm and adjustable power density until 1 W cm−2. The speckle patterns obtained from the bottom composite surfaces were monitored using a CCD camera without lens. The speckle field is recorded in a digital picture and stored by CCD camera as the carrier of information on the displacement of the tested surface. The calculated values were obtained for each pair of adjacent patterns and the changes in speckle contrast as a function of time were obtained from six repeated measurements. The speckle contrasts obtained from the bottom surface with 100 mW cm−1 were smaller than those than the other power densities. The higher power densities provided the higher shrinkage.

Aged composite resins ablation under different parameters of Er:YAG laser: ablation rate and morphological aspects

This work presents a study to understand the interaction between Er:YAG laser and composite resin. The main purpose is the development of a new ultra-conservative clinical technique: differential ablation for composite resin restorations using Er:YAG laser. A hybrid composite resin (Z100, 3M, USA) formatted as tablets recently cured was used and after artificial aging method the teeth were irradiated with a Er:YAG laser and two different conditions were considered, energy level per pulse (100, 300 and 500mJ), frequencies (5, 10 and 15Hz) and different water fluxes. Diameter and depth of each resulted microcavity was measured and the material removed volumes were calculated. The resulted values were plotted and feted to allow a comparative observation of the material removed as a function of energy level per pulse. Frequencies of 5 and 10Hz were similar between them and seemed to allow the highest material ablated volume, however considering the energy per pulse parameter, 300mJ was more efficient than the others, mainly when water flux was around 0.1ml/s. The highest water flux showed lower ablation rate with the lowest fluency. Water fluxes presented an important factor considering composite ablation, even because it could modulate depth and surface regularity of the irradiated material.

Optical characterization of one dental composite resin using bovine enamel as reinforcing filler

The use of composite resins for restorative procedure in anterior and posterior cavities is highly common in Dentistry due to its mechanical and aesthetic properties that are compatible with the remaining dental structure. Thus, the aim of this study was to evaluate the optical characterization of one dental composite resin using bovine enamel as reinforcing filler. The same organic matrix of the commercially available resins was used for this experimental resin. The reinforcing filler was obtained after the gridding of bovine enamel fragments and a superficial treatment was performed to allow the adhesion of the filler particles with the organic matrix. Different optical images as fluorescence and reflectance were performed to compare the experimental composite with the human teeth. The present experimental resin shows similar optical properties compared with human teeth.

Diffusion analysis of one photosensitizer in bovine teeth using fluorescence optical imaging

Some photosensitizers (PSs) used for PACT (Antimicrobial Photodynamic Therapy) show an affinity for bacterial walls and can be photo-activated to cause the desired damage. However, on dentine bacterias may be less susceptible to PACT as a result of limited penetration of the PS. The aim of this study was to evaluate the diffusion of one PS based on hematoporphyrin on dentine structures. Twelve bovine incisors were used. Class III cavities (3 x 3 x 1mm) were prepared on the mesial or distal surfaces using a diamond bur. Photogem® solution at 1 mg/mL (10 uL for each cavity) was used. The experimental Groups were divided according to thickness of dentine remaining and etched or no-etched before the PS application. The fluorescence excitation source was a VelScope® system. For image capture a scientific CCD color camera PixelFly® was coupled to VelScope. For image acquisition and processing, a computational routine was developed at Matlab®. Ficks Law was used to obtain the average diffusion coefficient of PS. Differences were found between all Groups. The longitudinal temporal diffusion was influenced by the different times, thickness and acid etching.