Dae Nyun Kim
Yonsei University
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Publication
Featured researches published by Dae Nyun Kim.
Acta Biomaterialia | 2011
Hyun Jong Lee; Dae Nyun Kim; Saemi Park; Yeol Lee; Won-Gun Koh
In this paper, we describe a simple method for fabricating micropatterned nanoporous substrates that are capable of controlling the spatial positioning of mammalian cells. Micropatterned substrates were prepared by fabricating poly(ethylene glycol) (PEG) hydrogel microstructures on alumina membranes with 200 nm nanopores using photolithography. Because hydrogel precursor solution could infiltrate and become crosslinked within the nanopores, the resultant hydrogel micropatterns were firmly anchored on the substrate without the use of adhesion-promoting monolayers, thereby allow tailoring of the surface properties of unpatterned nanoporous areas. For mammalian cell patterning, arrays of microwells of different dimensions were fabricated. These microwells were composed of hydrophilic PEG hydrogel walls surrounding nanoporous bottoms that were modified with cell-adhesive Arg-Gly-Asp (RGD) peptides. Because the PEG hydrogel was non-adhesive towards proteins and cells, cells adhered selectively and remained viable within the RGD-modified nanoporous regions, thereby creating cellular micropatterns. Although the morphology of cell clusters and the number of cells inside one microwell were dependent on the lateral dimension of the microwells, adhered cells that were in direct contact with nanopores were able to penetrate into the nanopores by small extensions (filopodia) for all the different sizes of microwells evaluated.
Sensors and Actuators B-chemical | 2009
Dae Nyun Kim; Yeol Lee; Won-Gun Koh
Polymers for Advanced Technologies | 2008
Yeol Lee; Dae Nyun Kim; Dongkil Choi; Woojin Lee; Jin-Won Park; Won-Gun Koh
Reactive & Functional Polymers | 2009
Sami Park; Yeol Lee; Dae Nyun Kim; Sangphil Park; Eunji Jang; Won-Gun Koh
Journal of Industrial and Engineering Chemistry | 2009
Dae Nyun Kim; Jin-Won Park; Won Gun Koh
한국생물공학회 학술대회 | 2009
Seung Hee Nam; Yoel Lee; Dae Nyun Kim; Sang Phil Park; Eunji Jang; Seami Park; Hyun Jong Lee; Won-Gun Koh
한국생물공학회 학술대회 | 2009
Saemi Park; Yeol Lee; Dae Nyun Kim; Sangphil Park; Eunji Jang; Hyun Jong Lee; Seung Hee Nam; Won-Gun Koh
한국생물공학회 학술대회 | 2009
Hyun Jong Lee; Yeol Lee; Dae Nyun Kim; Sang Phil Park; Saemi Park; Eunji Jang; Seung Hee Nam; Won-Gun Koh
한국생물공학회 학술대회 | 2008
Sangphil Park; Yeol Lee; Dae Nyun Kim; Eunji Jang; Saemi Park; Won-Gun Koh
한국생물공학회 학술대회 | 2008
Dae Nyun Kim; Hyun Jong Lee; Woojin Lee; Yeol Lee; Eun Ji Jang; Sang Phil Park; Saemi Park; Won-Gun Koh