Danilo Barral de Araújo

Estudo in vitro e ex vivo da ação de diferentes concentrações de extratos de própolis frente aos microrganismos presentes na saliva de humanos

This study evaluated in vitro and ex vivo, the action of propolis extracts in different concentrations, in comparison with the effectiveness of oral antiseptics, against microorganisms present in human saliva. For in vitro experiences, glucose 25% solution was added to saliva, then individual samples were collected to prepare a control group (C) and experimental groups (Exp), with addition of propolis extracts in 11%, 20% and 30%, and extracts of Periogard, Listerine, Malvatricin e Parodontax. For ex vivo experiments, samples of saliva in fast and after mouthrinses with extracts of propolis 11%, 20% and 30% were collected and later glucose solution was added. In both phases of the experiments, successive samples of the mixtures were collected and glucose consumption by microorganisms present in human saliva was determined by the glucose oxidase method in 0, 24 and 48 h of incubation preserved at 37 oC. The values obtained in control group proved that there was consumption of glucose by microorganisms after 24 and 48 h of incubation in both phases of the study. However, among experimental groups no significant differences have been observed. We conclude that the propolis 11%, 20% and 30% solutions showed the same antimicrobial action. As a result, it is justified to recommend the use of the 11% concentration. It was concluded that propolis had similar pharmacological effect when compared to the industrialized oral antiseptics tested.

In vitro study on tooth enamel lesions related to whitening dentifrice

BACKGROUND The tooth whitening substances for extrinsic use that are available in Brazil contain hydrogen peroxide or carbamide peroxide. Several studies have attributed the appearance of lesions in the enamel morphology, including hypersensitivity, to these substances. Such lesions justify fluoride therapy and application of infrared lasers, among other procedures. However, there is no consensus among researchers regarding the relevance of the severity of lesions detected on the tooth surface. OBJECTIVES The present study was carried out with an aim of evaluating in vitro the effects of the hydrogen peroxide, carbamide peroxide and sodium bicarbonate contained in dentifrice formulations, on human tooth enamel. MATERIALS AND METHODS After darkening process in laboratory, human premolars were brushed using dentifrice containing the two whitening substances (Rembrandt - carbamide peroxide and Mentadent - hydrogen peroxide) and the abrasive product (Colgate - sodium bicarbonate). The degree of specimen staining before and after this procedure was determined using spectrophotometry. Scanning electron microscopy (SEM) was used to obtain images, which were analyzed to show the nature of the lesions that appeared on the enamel surface. RESULTS The effectiveness of the whitening caused by hydrogen peroxide and carbamide peroxide and the abrasion caused by bicarbonate were confirmed, given that the treated test pieces returned to their original coloration. Based on SEM, evaluation of the enamel surfaces subjected to the test products showed that different types of morphologic lesions of varying severity appeared. CONCLUSIONS Whitening dentifrice containing hydrogen peroxide and carbamide peroxide produced lesions on the enamel surface such that the greatest sequelae were associated with exposure to hydrogen peroxide.

Fluxo e Capacidade de Tamponamento Salivar em Pacientes com Doenças Cardiovasculares

Resumen pt: Introducao: as doencas cardiovasculares sao a principal causa de morte em muitos paises. A influencia dos medicamentos utilizados pelos cardiopatas poder...

Análise das Características de Produtos Contendo Aspartame Comercializados em Salvador, Bahia, Brasil

Objective: To analyze the characteristics of aspartame and the information provided by the manufacturers of products containing this sweetener as compared to...

Vocational training of dentistry students regarding knowledge of oral rinses with antiseptic

Objective : the indiscriminate use of mouthwash with antiseptic carries health risks because the active pharmacological ingredients and other components of these formulations are not innocuous. From this perspective, it is essential to add this knowledge to the vocational training curriculum for dental students. The objective of this study was to assess the level of knowledge acquired by dental students graduating in 2014 about mouthwash with antiseptic and to evaluate the university education in three public and three private institutions. Methodology : an investigative enforcement tool was used to collect information from graduating students in 2014 from six dentistry undergraduate institutions in the state of Bahia. Results : of the students in private institutions, 56% reported having studied the rinses during their program, while 67% of students in public institutions indicated that they had not. Respondents cited a range of course and disciplines that addressed the topic. Of the students surveyed, 53.1% from public institutions and 46.9% from private institutions reported that they use rinses on a daily basis and expressed a preference for Colgate (50.7%), Oral B (26.4%) and Listerine (16.4%). With regard to knowledge of the active ingredients, 81% students did not answer, despite having general knowledge of diluents. Conclusion : although mouthwash is often included in the course of study, the level of knowledge students achieved was not considered satisfactory, given the high percentage of blank answers regarding active ingredients and indications.

A comparative study of the effects of two carbamide peroxide bleaching agents on the structure of enamel

Objective: Considering that different sources of carbamide peroxide bleaching agent may cause greater or lesser damage to the enamel and may have reduced effectiveness in dental whitening, the aim of this study was to compare the effects on the structure of enamel of two bleaching agents, drugstore-compounded and commercial carbamide peroxide bleaching agents. Methods: The specimens in the first group (drugstore-compounded carbamide peroxide) and second group (commercial carbamide peroxide) were treated with to 16% carbamide peroxide for 4 h per day for 20 days. The third group served as a control and was kept in artificial saliva during the test period. After the treatments, all specimens were examined via scanning electron microscopy. Results: Changes in the enamel morphology were similar in both experimental groups, but these changes were greater than those observed in the control group. The changes were mild and included only pores. Conclusion: There were no differences regarding damage to the enamel between drugstore-compounded and commercial carbamide peroxide bleaching agents.