Danilo Rheinheimer dos Santos

Fatores que afetam a disponibilidade do fósforo e o manejo da adubação fosfatada em solos sob sistema plantio direto

The basic phenomena of phosphorus dynamics in soil is important to define the rate and application manners of phosphates fertilizers. The forms and availability of phosphorus vary with the soil characteristics and properties. In young soils, the calcium phosphates are the main source to organisms. On the other hand, in highly weathered soils the organic phosphorus cycling assumes great importance in the maintenance of the biological bioavailability, although it is insufficient to economical crop productivity. In tropical and subtropical cultivated soils, the soil phosphorus in solution is controlled by phosphorus adsorbed at functional groups of inorganic colloids. The phosphorus diagnosis is made by soils analysis at different methodologies, each one with their advantages and limitations. The decision on adding phosphate and to the rate define is more dependent on the calibration between the value extracted with the crop yield than the extraction method. The elevation of phosphorus availability can be made in corrective or gradual addition. The corrective fertilization is more efficient, and maybe the only alternative, for phosphorus deficiency correction in deeper soil layers. The correction of phosphorus deficiency in deeper layers after the no-tillage system adoption is difficult. The superficial phosphate application in no-tillage seems not to be a good economical alternative and can become an environmental problem. The research must be intensified focusing among others: (a) phosphorus fractions in soil and crop yield; (b) phosphates fertilizers application in no-tillage system; (c) relation of phosphorus availability with exchangeable aluminum and compacted soil layers in soil deep; (d) monitoring of transferred phosphate from soil to aquatic systems.

Alterações no teor de fósforo no solo com aplicação de dejetos líquidos de suínos

Pig slurry serves as plant nutrient source, but may cause phosphorus accumulation in the soil when used improperly, which later can be transferred to the aquatic environment causing eutrophication. The objective of this study was to evaluate the potential risk of environmental contamination by continuous use of pig slurry, measuring alterations in phosphorus content in the soil and in the sorption isotherms. The study was carried out at the Universidade Federal de Santa Maria, in a Typic Hapludalf. The effects of applying 0, 40 and 80 m3 ha-1 pig slurry broadcast over the soil surface before sowing of each species in a crop rotation were studied. The pig slurry, applied on the soil surface under no tillage, contained phosphorus quantities superior to those exported by crops, which increased the available soil phosphorus to a depth of 15 cm. The saturation of the soil phosphate adsorption sites, as indicated by the parameters of the Langmuir equation, was proportional to the pig slurry rate. The pig slurry application altered the concentration of phosphorus in the equilibrium solution, the amount of phosphorus desorbed with water and the constant describing the affinity of phosphate for the substratum surface. The observed changes point to a risk of phosphorus desorption in case the sediments are transferred to surface water bodies.

Forms of inorganic phosphorus in soil under different long term soil tillage systems and winter crops

O cultivo de plantas com diferentes habilidades de absorcao e utilizacao de P sob diferentes sistemas de preparo de solo por um longo periodo de tempo pode alterar a ciclagem e a distribuicao das formas de P inorgânico no solo, como resultado de maior ou menor eficiencia de utilizacao do P adicionado via fertilizantes. O objetivo deste trabalho foi avaliar o efeito de longo periodo de cultivo de diferentes especies de inverno sob diferentes sistemas de preparo de solo na distribuicao das formas de P inorgânico do solo. Em 1986, foi estabelecido o experimento com seis tratamentos de inverno (tremoco-azul, ervilhaca-peluda, aveia-preta, nabo forrageiro, trigo e pousio) implantados num Latossolo Vermelho aluminoferrico na regiao sudoeste do Parana, sob sistema plantio direto (SPD) e sistema de cultivo convencional (SCC). A adicao de fertilizantes fosfatados na linha de semeadura no SPD promoveu aumento de P inorgânico, nas formas labeis e moderadamente labeis, e o revolvimento do solo no SCC redistribuiu o P aplicado em profundidade, promovendo aumento no teor de P moderadamente labil no solo das camadas subsuperficiais. A aveia-preta e o tremoco-azul foram mais eficientes na ciclagem de P e, quando cultivados sob SPD, aumentaram o teor de P labil no solo das camadas superficiais.

Pesticide contamination of water alters the metabolism of juvenile silver catfish, Rhamdia quelen

We investigated how pesticide contamination of water affects the metabolism of the silver catfish, Rhamdia quelen, by studying fish maintained at two sites with low and high anthropic activity (Lino Creek, southern Brazil). Several pesticides were found at both stream sites. After 30 days plasma glucose levels were higher in fish exposed to water in the low anthropic activity site than those exposed to water in the high anthropic activity site. Plasma K+ levels, however, were lower after exposure to low anthropic water than after exposure to high anthropic water. Moreover, values of hepatic glycogen, muscle lactate and protein were higher, but glycogen and protein of the kidney were lower in fish exposed to water at the high anthropic activity site. Our results show that these fish can be used as pesticide toxicity indicators in streams near agricultural fields.

Phosphorus accumulation and pollution potential in a hapludult fertilized with pig manure

Successive applications of pig litter to the soil surface can increase the phosphorus (P) content and alter its adsorption, promoting P transfer to surface or subsurface waters. The purpose of this study was to evaluate P accumulation and the pollution potential of a soil after application of pig litter. In March 2010, eight years after the installation of an experiment in Braco do Norte, Santa Catarina, SC, Brazil, on a Typic Hapludult, soil was sampled (layers 0-2.5, 2.5-5, 5-10, 10-15, 15-20 and 20-30 cm) after the following fertilization treatments: no pig litter fertilization, pig slurry application and pig manure application. In this period, 694 and 1,890 kg P2O5 ha-1 were applied in the treatments with pig slurry and pig manure, respectively. The P content was determined, based on Mehlich-1, anion exchange resin (AER), 0.01 mol L-1 CaCl2 and total P in the samples. The adsorption isotherm parameters were also determined by the Langmuir and Koski-Vahala & Hartikainem models in the layers 0-2.5 and 20-30 cm. The application of 1,890 kg P2O5 ha-1 in the form of pig manure led to P accumulation, as evidenced by Mehlich-1, down to a depth of 15 cm, by AER and 0.01 mol L-1 CaCl2 down to 20 cm and by total P to 30 cm. After application of 1,890 kg P2O5 ha-1 in the form of pig manure, the values of maximum P adsorption capacity were lowest in the deepest layer (20-30 cm), indicating the occupation of part of the adsorption sites of the particles. The application of swine manure to the soil over eight years increased the P quantity in the soil solution of the surface layer, indicating environmental contamination risk for surface and subsurface waters.

FÓSFORO MICROBIANO DO SOLO SOB SISTEMA PLANTIO DIRETO EM RESPOSTA À ADIÇÃO DE FOSFATO SOLÚVEL

The phosphorus cycle in the soil is controlled by physicochemical processes, such as adsorption and desorption, and by biological processes, such as immobilization and mineralization. This study was carried out to evaluate the seasonal variation of phosphorus in the soil microbial biomass (Pm) as related to rates and timing of soluble phosphate application. The experiment was carried out in May 2002 on an Oxisol under no-till for seven years, in Ibiruba, in the southern Brazil. Five rates of soluble phosphate (0, 40, 80, 120, and 160 kg ha-1 of P2O5) were applied twice in a Lolium multiflorum/Glicine max cultivation cycle; the first one in the winter at Lolium multiflorum sowing and the second in the summer at Glicine max sowing. Microbial phosphorus and available phosphorus by anion exchange resin were evaluated in the 0-10 cm soil layer 91, 133, 147, 203, and 267 days after Lolium multiflorum sowing. Phosphate application increased microbial phosphorus, more intensely when applied at soybean sowing over the Lolium multiflorum residues. P immobilization in the microbial biomass was temporary and decreased over the course of the crop cycle; these variations were not correlated with variations in P extracted by anion-exchange resin.

Combining visible-based-color parameters and geochemical tracers to improve sediment source discrimination and apportionment.

Parameter selection in fingerprinting studies are often time-consuming and costly because successful fingerprint properties are generally highly site-specific. Recently, spectroscopy has been applied to trace sediment origin as a rapid, less expensive, non-destructive and straightforward alternative. We show in this study the first attempt to combine both geochemical tracers and color parameters derived from the visible (VIS) spectrum in a single estimate of sediment source contribution. Moreover, we compared the discrimination power and source apportionment using VIS-based-color parameters and using the whole ultra-violet-visible (UV-VIS) spectrum in partial last square regression (PLSR) models. This study was carried out in a small (1.19 km(2)) rural catchment from southern Brazil. The sediment sources evaluated were crop fields, unpaved roads, and stream channels. Color parameters were only able to discriminate unpaved roads from the other sources, disabling its use to fingerprint sediment sources itself. Nonetheless, there was a great improvement in source discrimination combining geochemical tracers and color parameters. Unlike VIS-based-color parameters, the distances between sediment sources were always significantly different using the whole UV-VIS-spectrum. It indicates a loss of information and, consequently, loss of discriminating power when using VIS-based-color parameters instead of the whole UV-VIS spectrum. Overall, there was good agreement in source ascription obtained with geochemical tracers alone, geochemical tracers coupled with color parameters, and UV-VIS-PLSR models, and all of them indicate clearly that the main sediment source was the crop fields, corresponding to 57 ± 14, 48 ± 13, and 62 ± 18%, respectively. Prediction errors for UV-VIS-PLSR models (6.6 ± 1.1%) were very similar to those generated in a mixed linear model using geochemical tracers alone (6.4 ± 3.6%), but the combination of color parameters and geochemical tracers decreases the prediction error (5.4 ± 2.0%). Therefore, the use of VIS-based-color parameters combined to geochemical tracers can be a rapid and inexpensive way to improve source discrimination and precision of sediment source apportionment.

Using a two site-reactive model for simulating one century changes of Zn and Pb concentration profiles in soils affected by metallurgical fallout.

Predicting the transfer of contaminants in soils is often hampered by lacking validation of mathematical models. Here, we applied Hydrus-2D software to three agricultural soils for simulating the 1900-2005 changes of zinc and lead concentration profiles derived from industrial atmospheric deposition, to validate the tested models with plausible assumptions on past metal inputs to reach the 2005 situation. The models were set with data from previous studies on the geochemical background, estimated temporal metal deposition, and the 2005 metal distributions. Different hypotheses of chemical reactions of metals with the soil solution were examined: 100% equilibrium or partial equilibrium, parameterized following kinetic chemical extractions. Finally, a two-site model with kinetic constant values adjusted at 1% of EDTA extraction parameters satisfactory predicted changes in metal concentration profiles for two arable soils. For a grassland soil however, this model showed limited applicability by ignoring the role of earthworm activity in metal incorporation.

Modeling field-scale vertical movement of zinc and copper in a pig slurry-amended soil in Brazil

Organic amendments often represent a source of trace metals (TMs) in soils, which may partly leach into the groundwater. The objectives of this study were (1) to validate Hydrus-2D for modeling the transport of Zn and Cu in an Alfisol amended with pig slurry (PS) by comparing numerical simulations and experimental field data, and (2) to model the next 50 years of TM movements under scenarios of suspended or continued PS amendments. First, between 2000 and 2008, we collected detailed Zn and Cu data from a soil profile in Santa Maria, Brazil. Two hypotheses about Zn and Cu reactivity with the solid phase were tested, considering physical, hydraulic, and chemical characteristics of six soil layers. Using a two-site sorption model with a sorption kinetic rate adjusted based on laboratory EDTA extractions, Hydrus simulations of the vertical TM transport were found to satisfactorily describe the soil Zn and Cu concentration profiles. Second, the long-term fate of Zn and Cu in the soil was assessed using the validated parameterized model. Numerical simulations showed that Zn and Cu did not present risks for groundwater pollution. However, future Cu accumulation in the surface soil layer would exceed the Brazilian threshold for agricultural soils.

Copper and zinc accumulation and fractionation in a clayey Hapludox soil subject to long-term pig slurry application

Pig slurry (PS) recycling as fertilizer is commonly practiced as an option for minimizing livestock waste. Successive PS applications on the soil can lead to crop toxicity and environmental risk. Despite extensive investigation of macronutrient behavior, the fate of trace metals remains uncertain and only a few long-term field studies have been reported to date. This study was designed to assess the impact of 11-year continuous PS spreading on Cu and Zn accumulation and fractionation in a Brazilian clayey Hapludox soil. Three different PS application rates--50, 100 and 200m3 ha(-1)year(-1)--were monitored at six soil depths in comparison to a non-amended control soil. The modified Geological Survey of Canada sequential extraction protocol was applied. A statistically significant increase in Cu and Zn total concentration (assessed by the sum of fractions) was noted only within the 0-5 cm soil layer for the 50 and 100 m3 ha(-1) year(-1) (PS50 and PS100) treatments, and up to 10-15 cm for the 200 m3 ha(-1)year(-1) (PS200) treatment. The mass balance, determined for the 22 PS amendments over the period, confirmed the overall exogenous Cu and Zn accumulation within the surface layers. More than 70% of the natural heavy metal content was originally in the residual fraction. However, this was the only fraction not influenced by the PS amendments. After PS input, the exogenous Cu was mainly detected in the fraction bound to organic matter (66.4%) within the PS200 0-5 cm soil layer. Exogenous Zn was mainly distributed between the adsorbed fraction (36.7%) and the organic matter fraction (32.0%) within the PS200 0-5 cm layer.