Danswell Starrs

All in the ears: unlocking the early life history biology and spatial ecology of fishes

Obtaining biological and spatial information of the early life history (ELH) phases of fishes has been problematic, such that larval and juvenile phases are often referred to as the ‘black box’ of fish population biology and ecology. However, a potent source of life‐history data has been mined from the earstones (otoliths) of bony fishes. We systematically reviewed 476 empirical papers published between 2005 and 2012 (inclusive) that used otoliths to examine fish ELH phases, which has been an area of increasing attention over this period. We found that otolith‐based research during this period could be split into two broad themes according to whether studies examined: (i) biological objectives related to intrinsic processes such as larval and juvenile age, growth and mortality, and/or (ii) spatial objectives, such as habitat use, dispersal and migration. Surprisingly, just 24 studies (5%) explored a combined biological–spatial objective by simultaneously exploiting biological and spatial information from otoliths, suggesting much more scope for such integrated research objectives to be answered via the use of multiple otolith‐based techniques in a single study. Mapping otolith analytical techniques across these two approaches revealed that otolith structural analysis was mainly used to investigate biological processes, while otolith chemical analyses were most often applied to spatial questions. Heavy skew in research effort was apparent across biomes, with most (62%) publications specific to marine species, despite comparable levels of species richness and the importance of freshwater taxa (just 15% of papers). Indeed, around 1% (380 species) of a possible 31400+ extant species were examined in our surveyed papers, with a strong emphasis on temperate marine species of commercial value. Potential model species for otolith‐based ELH ecology research are arising, with the eel genus Anguilla (24 studies) and the European anchovy Engraulis encrasicolis (14 studies) attracting more research effort than most other taxa. While there is a preponderance of common techniques (e.g. daily otolith increment counts, increment widths), novel techniques such as transgenerational marking and computed X‐ray tomography, are increasingly being applied in published studies. The application of an integrative approach based on a combination of emerging techniques and traditional methods holds promise for major advances in our understanding of ELH fish ecology and to shine light into the ‘black box’ of fish ecology.

The importance of protein in leaf selection of folivorous primates.

Protein limitation has been considered a key factor in hypotheses on the evolution of life history and animal communities, suggesting that animals should prioritize protein in their food choice. This contrasts with the limited support that food selection studies have provided for such a priority in nonhuman primates, particularly for folivores. Here, we suggest that this discrepancy can be resolved if folivores only need to select for high protein leaves when average protein concentration in the habitat is low. To test the prediction, we applied meta‐analyses to analyze published and unpublished results of food selection for protein and fiber concentrations from 24 studies (some with multiple species) of folivorous primates. To counter potential methodological flaws, we differentiated between methods analyzing total nitrogen and soluble protein concentrations. We used a meta‐analysis to test for the effect of protein on food selection by primates and found a significant effect of soluble protein concentrations, but a non‐significant effect for total nitrogen. Furthermore, selection for soluble protein was reinforced in forests where protein was less available. Selection for low fiber content was significant but unrelated to the fiber concentrations in representative leaf samples of a given forest. There was no relationship (either negative or positive) between the concentration of protein and fiber in the food or in representative samples of leaves. Overall our study suggests that protein selection is influenced by the protein availability in the environment, explaining the sometimes contradictory results in previous studies on protein selection. Am. J. Primatol. 79:e22550, 2017.

Longevity in maternal transmission of isotopic marks in a tropical freshwater rainbowfish and the implications for offspring morphology

Transgenerational marking is increasingly being used to study the early life history, biology and ecology of fishes. However, the timeframe over which the injected enriched stable isotopes remain in the mother and are passed onto her offspring is largely unknown. Similarly, we have relatively little knowledge of the effects of isotope labelling on the morphology of offspring. In this study, we injected adult female eastern rainbowfish (Melanotaenia splendida) with two doses (20mgg � 1 and 40mgg � 1 ) of enriched 137 Ba or 87 Sr stable isotopes to mark the otoliths of their larvae and examine the effects of isotope labelling on larvae morphology. Isotope ratios in larval otoliths were significantly different from controls in larvae hatched up to 174 days post-injection, indicating that enriched isotopes can mark the larvae of this daily spawningspeciesupto6monthsafterasingleinjection.Isotope-markedlarvaedisplayedvariable,butgenerallyincreased physical size, indicating that enriched stable isotope labelling may have some unintended effects on larvae morphology. Consequently, transgenerational marking provides a long-term tool for marking the offspring of M. splendida to disentangle their patterns of survivorship and dispersal, with the caveat that such studies should be interpreted in light of potential isotope-related changes in offspring morphology. Additional keywords: early life history, fitness, LA-ICPMS, mark-recapture, Melanotaenidae.

Can backcalculation models unravel complex larval growth histories in a tropical freshwater fish

This experimental study compared the precision and accuracy of the biological intercept (BI), modified fry (MF) and time-varying growth (TVG) backcalculation models in estimating the early growth of the tropical freshwater purple-spotted gudgeon Mogurnda adspersa. Larvae were reared up to 41 days post hatching under two temperatures and four different feeding regimes. Food and temperature treatments induced complex growth profiles among fish, and although total length (LT ) and otolith radius were related under all conditions, some uncoupling was evident in the otolith-somatic-growth (OSG) relationship of fish subjected to periods of changing food availability. Furthermore, otolith growth was found to be significantly influenced by temperature, but not by food availability. Analysis of backcalculation residuals by linear mixed effects modelling revealed that BI and TVG were equally precise in predicting somatic growth, with the highest accuracy provided by TVG. The performance of all the three models declined as the OSG relationship weakened under low-food conditions, with maximum errors estimated to be 39, 60 and 36% of observed LT for the BI, MF and TVG models, respectively. The need for careful validation of backcalculation models is emphasized when examining fishes subjected to variable environmental conditions, and when exploring the differential influence of temperature and food on fish LT and otolith growth.

Distribution and habitat preference of the broad-toothed rat (Mastacomys fuscus) in the Australian Capital Territory, Australia

The broad-toothed rat (Mastacomys fuscus) is a small to medium-sized rodent found in south-eastern Australia. Recent surveys across the southern portion of its range indicate that the species is in decline, and climate change has been identified as a key threat to the localised persistence of this species. The present study reports on a rapid field survey across 14 high montane and subalpine sites (including moist tussock grassland, sedgelands, heathlands and bogs) in the southern Australian Capital Territory, Australia. M. fuscus scats were recorded along transects, and habitat, vegetation, distance to drainage lines and disturbance due to feral animals were recorded. Relative abundance of M. fuscus was positively related to specific vegetation types (heath, sedge and Poa) and site size. Conversely, relative abundance of M. fuscus was negatively related to disturbance due to feral animals, and distance from creek drainage lines. This study indicates that M. fuscus has specific habitat preferences and threats associated with environmental change and introduced species may threaten populations in the Australian Capital Territory.

Foliar Nutritional Quality Explains Patchy Browsing Damage Caused by an Invasive Mammal.

Introduced herbivores frequently inflict significant, yet patchy damage on native ecosystems through selective browsing. However, there are few instances where the underlying cause of this patchy damage has been revealed. We aimed to determine if the nutritional quality of foliage could predict the browsing preferences of an invasive mammalian herbivore, the common brushtail possum (Trichosurus vulpecula), in a temperate forest in New Zealand. We quantified the spatial and temporal variation in four key aspects of the foliar chemistry (total nitrogen, available nitrogen, in vitro dry matter digestibility and tannin effect) of 275 trees representing five native tree species. Simultaneously, we assessed the severity of browsing damage caused by possums on those trees in order to relate selective browsing to foliar nutritional quality. We found significant spatial and temporal variation in nutritional quality among individuals of each tree species examined, as well as among tree species. There was a positive relationship between the available nitrogen concentration of foliage (a measure of in vitro digestible protein) and the severity of damage caused by browsing by possums. This study highlights the importance of nutritional quality, specifically, the foliar available nitrogen concentration of individual trees, in predicting the impact of an invasive mammal. Revealing the underlying cause of patchy browsing by an invasive mammal provides new insights for conservation of native forests and targeted control of invasive herbivores in forest ecosystems.

Ceasefire: minimal aggression among Murray River crayfish feeding upon patches of allochthonous material

Abstract. Transport and processing of allochthonous material is crucial for trophic pathways in headwater streams. Freshwater crayfish are known to affect and exploit the break-down of in-stream terrestrial plant material into detritus. We recorded Euastacus armatus (Murray River crayfish) individuals feeding on discrete patches of allochthonous material within an unregulated section of the Goodradigbee River, an upland stream in temperate Australia. Despite suggestions of aggressive territoriality, E. armatus were observed by remote and manual underwater filming to feed in non-aggressive aggregations on these piles of fine woody debris and leaf litter. On the basis of observations of 25 individuals found in the vicinity of the allochthonous patches, this population comprised mostly female individuals at smaller sizes of maturity than has been recorded for lowland populations of E. armatus. Our study confirms the importance of concentrated allochthonous food patches for detritivores, and points to the important trophic linkage between terrestrial and aquatic ecosystems via a widespread and iconic freshwater invertebrate. Moreover, these non-aggressive feeding aggregations of E. armatus challenge notions of aggression in this species that have been developed in small-scale aquarium studies.

Coal grunters shift benthic objects to access macroinvertebrates in a headwater stream

Few fishes have been documented shifting objects to access prey. Coal grunters were observed using the mouth, snout or nape to lift, flip or roll benthic objects and expose benthic macroinvertebrates during a series of daytime snorkelling sessions in a clear, sandy upland tributary of the Mitchell River, northern Queensland, Australia.