Deng Tiansheng

Antioxidant Properties of Isolated Isorhamnetin from the Sea Buckthorn Marc

In the present study, the process of separation and purification of isohamnetin from marc of sea buckthorn was obtained. The antioxidant properties of the pure isolated isorhamnetin were evaluated by the scavenging of the diphenylpicrylhydrazyl radical (DPPH), iron (III) to iron (II)—reducing, and iron-chelating assays. High purity isorhamnetin (92.1%) was obtained and the results of antioxidant assays showed that isorhamnetin performed significantly compared with ascorbic acid and BHT, and the linear correlations were good in these assays. In conclusion, isorhamnetin may have potential as a natural antioxidant to alternate synthetic substances as food additive with its antioxidant activity.

Extraction and Purification of Solanesol from Tobacco Extract

In order to develop a purification procedure for solanesol from tobacco extract without column chromatography,the effects of the dosage of NaOH and alcohol in saponification,saponification time,dewaxing solvent,crystallization solvent and crystallization time on the extraction and purification of solanesol were investigated.The determined optimum procedure was:tobacco extract in anhydrous alcohol was saponified at 65 ℃ for 4 hours at the presence of 4%NaOH(mass/mass),then neutralized with concentrated hydrochloric acid,filtrated for dewaxing,crystallized in methanol at-18 ℃ for 6 hours twice and recrystallized in acetoni[JP3]trile twice.5.1 g of white solid(solanesol concentration 90.5%,mp 41.5 ℃)was obtained from 50 g tobacco extract containing 7.4% of solanesol by the procedure.This procedure could be used to isolate solanesol from tobacco extract.