Dibyajyoti Talukdar

The Role of Estrogen and Progesterone Hormone on Vaginal Cytology in Bitch

The aim of the present study was to determine the stages of the reproductive cycle of the bitch by using the exfoliative vaginal cytology technique and its relationship with hormonal level. A total of 12 bitches (n=12) were selected and vaginal cytology along with hormonal estimation was done to identify the different stages of the reproductive cycle. The vaginal samples were collected as per the cotton swab technique and stained with Leishman stain and examined microscopically for differential count of exfoliated vaginal epithelial cells. On the same day blood samples were also collected aseptically for hormonal estimation. The percentage of parabasal cell, intermediate cell and superficial cells were 7.04±0.28, 22.33±0.28, and 62.32±0.28 in proestrus; 0.56±0.03, 5.68±0.03 and 92.29±0.03in estrus and 32.56±0.28, 35.08±0.28 and 12.83±0.28 in diestrus respectively. Vaginal smears were characterized by presence of massive numbers of erythrocytes and disappearance of neutrophils in proestrus, clear background of smear and absence of neutrophils with scanty erythrocytes in estrus, infiltration of neutrophils and dirty background in diestrus. The estrogen level (29.50 ±0.28) was statistically high during proestrus and highest progesterone level (39.50 ±0.28) during diestrus. On conclusion, hormonal changes in different stages of reproductive cycle presented mainly by the effect of elevated estrogen hormone that associated with increase in superficial cells count and cornification, with reduction in neutrophil and parabasal cell in estrus and Progesterone hormone showed increased towards diestrus level with increasing intermediate cells.

Reproductive behaviour and performance of estrus synchronized Hampshire crossbred pigs

A total of 62 apparently healthy cyclic Hampshire crossbred pig (7 months to 2 years) maintained at All India Co-ordinated Research Project on pig, AAU, Khanapara were used in the present study. Thirty two gilts and 18 sows were injected i/m with 400IU PMSG (Folligon) + 200IU hCG (Chorulon). The percentage of pigs showing synchronized oestrus was 62.50 in gilts and 33.33 in sows. The mean interval from oestrus synchronization treatment to onset of oestrus, duration of oestrus, gestation length, litter size, litter weight, still birth and runt piglet in synchronized gilts and sows were 3.00+0.15 and 4.00+0.58 days, 81.60+3.49 and 112.00+4.00 hrs, 113.67+0.29 and 114.33+0.33 days, 11.66+1.41 and 12.66+0.33, 10.62+0.36 and 13.73+0.41 kg, 7.62 and 2.60 and 4.75 and 0.00 respectively. The duration of oestrus in both gilts and sows was longer in treated animals than in untreated control. Intensity of oetrus was more following synchronization treatment in both gilts and sows as compared to that in untreated control animals. In conclusion 400IU PMSG + 200IU hCG can be used for synchronization of oestrus in gilts and sows.

Male Pseudohermaphroditism in a Bitch: A Case Report

An eight months old Cocker Spaniel bitch, weighing 11kg, was presented to the Department of Surgery and Radiology, College of Veterinary Sciences & A.H., CAU, Selesih, Mizoram, with the complaint of dsyuria. On physical examination of the external genitalia revealed that the bitch possessed the vulva with enlarged clitoris protruding from the vulvar juncture and both testes remained undescended. Laparotomy revealed persistent Mullerian duct (PMD) with abdominally located testicles. Thus the congenital defects were diagnosed as male pseudohermaphrodititism (MPH) with PMD.

Comparative Biochemical Parameters Studies on Pati and Chara-Chemballi Ducks (Anas Platyrhynchos Domesticus) During their Laying Periods -

The present investigation was undertaken for comparative study of blood biochemical constituents of Pati and Chara-Chemballi ducks in their laying period (42 weeks age). Twelve each of the ducks were procured from Barpeta district and SIRD of Assam and blood samples were collected from the wing veins from each bird under aseptic condition. The results showed that the serum glucose, ALT and ALP level was found to be significantly higher in Chara-Chemballi duck than Pati duck except serum calcium level which was significantly higher in Pati duck. The total protein of blood serum did not differ significantly in both the genetic groups of ducks.The variation between the two genetic groups might be due to difference in breeds, hormonal and other nutritional aspects. It is concluded that significant differences in blood biochemical profile among the two genetically different ducks could be considered as a references values and this may serve as a guide to assess the state of health in the monitored birds as well as it might be helping the physiologist, pathologist and poultry scientists for preparation of effective production strategy.

Management of Incomplete Uterine Inertia in a Labrador Bitch - A Case Report -

A Labrador bitch having fifty four days of pregnancy was presented at college clinics with the history of no sign of labor after birth of one pup. The bitch was found active with dilated cervix. Abdominal palpation and ultrasonographic imaging revealed presence of foetuses in the gravid uterus. The case was further diagnosed as incomplete uterine inertia. In the present case study it was noted that intravenous administration of oxytocin, calcium gluconate and dextrose saline as effective therapeutic agents to expelled the fetuses.