Dieter Walossek

Pentastomid parasites from the Lower Palaeozoic of Sweden

Small phosphatised fossils from late Cambrian limestones of Vastergotland, Sweden, share major external features with larval extant Pentastomida, such as a prominent head with two pairs of stumpy limbs adapted for attachment, and a slender trunk of four portions. Even such details, as paired forehead structures, pores on the inner edges of the head limbs and paired papillae at the rear of the trunk correspond with structures of extant pentastomid larvae. Neither the fossils nor the Recent pentastomids add any additional body segments during growth (segment constancy). Since characters of this kind and in this combination do not occur elsewhere, the fossils are recognised as true Pentastomida. Major differences, such as distinctly divided head limbs, partial occurrence of vestigial trunk limbs, and a different mode of trunk development during growth can be explained as representing merely the plesiomorphic state of characters of Pentastomida, indicating that the fossils are representatives of its stem-group prior to branching into the two Recent lineages. The fossils clearly document the marine origin of the Pentastomida, and that their specific morphology and parasitic life style were already established in the late Cambrian at a high degree of diversification, long before the terrestrialisation of their present final hosts, the tetrapods. General arthropod affinities are recognisable not least in the nature of the limbs, but the morphology of stem- and crown-group pentastomids gives no clues for closer relationship with any of the major (eu)arthropod taxa.

Larval development of the rhizocephalan cirripede Briarosaccus tenellus (Maxillopoda: Thecostraca) reared in the laboratory: A scanning electron microscopy study

SEM investigations of laboratory-reared larvae of Briarosaccus tenellus Boschma, 1970, revealed five naupliar instars, one more than previously described for the Rhizocephala. The external features of these and the cypris larva are described in detail. The youngest instar (NO) is characterised by a better developed furca than in subsequent stages and one additional antennulary seta. Differences in outline, shape of antennulae, and hind-body denticulation, offer the potential of individual discrimination. All the nauplii possess a transparent, hollow cuticular ring around their body. This ‘flotation collar’ represents the bulged margin of the larval head shield and is attached to the body along a delicate ridge. Three pairs of tiny pores in contact with the ridge possibly regulate inflation of the ring, but details of this mechanisms remain unknown. Due to total lecithotrophy, the nauplii of B. tenellus have limbs setation reduced to that needed for swimming only, and other feeding structures such as the labrum are also highly reduced. In the antennulae, the morphological changes in form and setation were followed from nauplius to cypris and shown to largely resemble events in ontogeny of the thoracican barnacle Semibalanus balanoides. On this basis we establish a homology scheme between antennulary setae in these two species. In both B. tenellus and S. balanoides, a naupliar seta, apically on the fourth antennulary segment develops into a conspicuous aesthetasc while one (B. tenellus) or two (S. balanoides) subapical and postaxially sited setae on the same segment develops into into four short setae in the cypris. Our study reveals that the terminology used in describing cirripede nauplius and cypris larvae is often misleading or even erroneous and improvements are suggested. Notably replacing ‘cypris carapace’ with the ontogenetically and phylogenetically more informative term ‘head shield’.

Limb ontogeny and trunk segmentation in Nebalia species (Crustacea, Malacostraca, Leptostraca)

Abstract The ’egg-larval’ development of two species of Nebalia has been examined with SEM. Various details concerning limb ontogeny and trunk segmentation are described. The most important of these are the following. The tripartite state of the peduncle of antenna 2 in the adult of Nebalia species is derived from the fusion of the third and fourth podomeres, present in late larvae. The proximal portion of the mandible in the adult of Nebalia brucei, carrying the ’coxal process’, is, based on the ontogenetic evidence, interpreted as the combined basis and coxa, and the bipartite palp is interpreted as the endopod. The early development of the thoracopods and the three anteriormost pleopods is identical. They all start as laterally directed, biramous limb buds. This suggests that tagmatisation of the trunk of the Leptostraca (and other Malacostraca) has been developed from an ancestor with an undivided trunk region with serially similar limbs. Certain early stages reveal an extra, ’eighth’, limbless pleon segment, as compared with the normal number of seven pleomeres of adult Leptostraca. The presence of a row of ventral, sternitic, triangular processes between the bases of the thoracopods, as they are found in certain stages of a species of Nebalia, is suggested as a possible ground pattern for the Malacostraca.