Dilek Öztürk

Molecular Characterization of ESBL-Producing Escherichia Coli Isolated from Healthy Cattle and Sheep

Abstract The present study aims to characterize ESBL-producing Escherichia coli isolated from healthy cattle and sheep in the Burdur province of Turkey. Fecal samples from a total of 200 cattle and 200 sheep were tested and ESBL-producing E. coli was isolated from 31 (15.5%) cattle and three (1.5%) sheep samples using the Clinical and Laboratory Standards Institute’s combined disk method. Among the ESBL gene classes detected by PCR, blaCTX-M was the most frequent type, followed by the blaTEM and blaSHV families. ESBL-producing E. coli isolates showed co-resistance to multiple classes of antibiotics including aminoglycosides, phenicols, quinolones, folate pathway inhibitors and tetracyclines. The resistance rates were higher in the cattle isolates than in the sheep isolates. Phylogenetic grouping of the E. coli isolates indicated group A (particularly A1) was the predominant phylogenetic group (19/34, 55.9%), followed by groups B1 (9/34, 26.5%) and D (6/34, 17.6%); none of the isolates belonged to group B2. The study shows that ESBL-producing E. coli isolates exist in the intestinal flora of healthy cattle and sheep in the Burdur province of Turkey. This is the first report showing the emergence of CTX-M type ESBL-producing E. coli in sheep farms in Turkey

Kedi ve Köpeklerden Dermatofitlerin İzolasyonu

Dermatofitozis, fungal etkenler tarafindan olusturulan, kil folikulleri, tirnak ve epidermisin keratin tabakasini etkileyen infeksiyoz ve zoonoz bir hastaliktir. Dermatofitozise neden olan etkenler Microsporum, Trichophyton ve Epidermophyton cinsinde bulunmaktadir. Bu calismada, 2013-2017 yillari arasinda ve farkli mevsimlerde kedi ve kopeklerden alinarak Mehmet Akif Ersoy Universitesi Veteriner Fakultesi Mikrobiyoloji Laboratuvari’na dermatofitozis supheli olarak gonderilen toplam 102 deri kazintisi ve kil ornegi incelendi. Orneklerin mikolojik analizi direkt mikroskopi ve Sabouraud Dekstroz Agara ekim yontemleri ile yapildi. Toplam 102 ornegin 56 (%54.90)’si dermatofitozis yonunden pozitif bulundu. Kedilerden alinan 24 materyalin 5 (%20.83)’inden Microsporum sp. , 3 (%12.5)’unden ise Trichophyton sp. izole edildi. Kopek orijinli 78 materyalde 47 (%60.25) Microsporum sp. ve 1 (%1.28) Trichophyton sp. teshis edildi. Kedi ve kopeklerde dermatofitozis olgularina cogunlukla ilkbaharda % 82.75 oraninda olmak uzere sonbahar (% 64.28) ve yaz (% 38.88) mevsimlerinde de rastlandi.