Donal F. Magee

Attempted Opacification of the Pancreas with the Use of Iodinated Dyes

The recent report by Robert Shapiro (3), of experimental opacification of the pancreas in the rabbit by intravenous injection of a contrast material, has prompted an account of some previous experiments of a similar nature. Approximately three years ago a study was initiated in which attempts were made to opacify the pancreas in the dog following intravenous injection of iodinated dyes. Our initial studies were undertaken following the report of Ingraham and Visscher (1) on the excretion of aniline and other dyes from the pancreatic duct of dogs. They found that within two or three hours of injection the external secretion of the pancreas might contain as much as 5 per cent of the blood concentration of some of these dyes. Wool blue G, fast fuchsin B, rhodamine B, acridine red, indigo carmine, eosin BS, basic fuchsin, fluorescein, and methyl red were excreted from the pancreatic duct in amounts varying from 0.5 to 5 per cent of the blood concentration within a very short period. For our first attempts to ...

Inhibition of Heidenhain Pouch Pepsin Secretion by Commercial Cholecystokinin and Duodenal Fat in Dogs

In unanesthetized dogs with Heidenhain pouches and separated duodenal pouches, intravenous infusion of commercial cholecystokinin (1.0 IDU per min) produced a significant depression of pouch acid and pepsin secretion stimulated by pentagastrin (1.0 microg per min) or by methacholine (2.0 microg per min). Acid response to methacholine was temporarily augmented. Irrigation of the duodenal pouches with emulsified fat produced similar patterns of depression of acid secretion in response to pentagastrin and pepsin secretion in response to pentagastrin or methacholine. Acid secretion stimulated by methacholine was temporarily augmented after the irrigation. It is concluded that fat releases endogenous cholecystokinin from the duodenal mucosa and that cholecystokinin, or duodenal fat, powerfully depresses Heidenhain pouch pepsin secretion in dogs. The involvment of the gastric inhibitory polypeptide (GIP) cannot be assessed from the present experiments.: In unanesthetized dogs with Heidenhain pouches and separated duodenal pouches, intravenous infusion of commercial cholecystokinin (1.0 IDU per min) produced a significant depression of pouch acid and pepsin secretion stimulated by pentagastrin (1.0 microg per min) or by methacholine (2.0 microg per min). Acid response to methacholine was temporarily augmented. Irrigation of the duodenal pouches with emulsified fat produced similar patterns of depression of acid secretion in response to pentagastrin and pepsin secretion in response to pentagastrin or methacholine. Acid secretion stimulated by methacholine was temporarily augmented after the irrigation. It is concluded that fat releases endogenous cholecystokinin from the duodenal mucosa and that cholecystokinin, or duodenal fat, powerfully depresses Heidenhain pouch pepsin secretion in dogs. The involvment of the gastric inhibitory polypeptide (GIP) cannot be assessed from the present experiments.

Gastric Acid and the Gastropancreatic Distension Reflex

Summary Distension of the stomach with 300 cc. of an alkaline solution increased the secretion of both water and protein by the pancreas. In only one instance did the pH of solutions used fall below 6 during the 10 or 20 minutes each solution remained in the stomach.