Donald F. Hunt
Merck KGaA
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Publication
Featured researches published by Donald F. Hunt.
Infection and Immunity | 2002
David C. Flyer; Venkatesh Ramakrishna; Cara Miller; Helen Myers; Melanie McDaniel; Karen Root; Caroline Flournoy; Victor H. Engelhard; David H. Canaday; Jarrod A. Marto; Mark M. Ross; Donald F. Hunt; Jeffrey Shabanowitz; Forest M. White
ABSTRACT Identification of Mycobacterium tuberculosis proteins that can provide immunological protection against tuberculosis is essential for the development of a more effective vaccine. To identify new vaccine targets, we have used immunoaffinity chromatography to isolate class I HLA-A*0201-peptide complexes from M. tuberculosis-infected cells and sequenced the isolated peptides by mass spectrometry. From this material, we have identified three peptides derived from a single M. tuberculosis protein that is encoded by the M. tuberculosis Rv0341 gene. Although no known protein encoded by the Rv0341 gene has been described, it is predicted to give rise to a 479-amino-acid protein with a molecular mass of 43.9 kDa. The three peptides identified are all nested and were found to be antigenic, in that they were capable of inducing peptide-specific, CD8+ T cells from healthy blood donors in vitro and capable of recognizing and lysing M. tuberculosis-infected dendritic cells. This methodology provides a powerful tool for the identification of M. tuberculosis proteins that can be evaluated as potential vaccine candidates.
Archive | 2001
David C. Flyer; Mark M. Ross; Donald F. Hunt; Forest M. White; Victor H. Engelhard; Ramila Philip
Archive | 2004
Els A. J. M. Goulmy; Donald F. Hunt; Victor H. Engelhard
Archive | 2005
Donald F. Hunt; Joshua J. Coon; John E. P. Syka; Jarrod A. Marto
Archive | 2005
Joshua J. Coon; Donald F. Hunt; John E. P. Syka
Archive | 2002
Donald F. Hunt; Forest M. White; Scott B. Ficarro
Archive | 2002
Olivera J. Finn; Henry Kao; Donald F. Hunt; Jarrod A. Marto
Archive | 2002
Donald F. Hunt; Forest M. White; Scott B. Ficarro
Archive | 1998
Victor H. Engelhard; Donald F. Hunt; David Kittlesen; Craig L. Slingluff
Archive | 1998
Craig L. Slingluff; Donald F. Hunt; Victor H. Engelhard; David Kittlesen