Donald Hnatowich
Keio University
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Publication
Featured researches published by Donald Hnatowich.
Molecular Imaging and Biology | 2006
Kayoko Nakamura; Yi Wang; Xinrong Liu; Atsushi Kubo; Donald Hnatowich
PurposeThe aim of the study is to determine whether delivery into tumor cells in vivo of a 99mTc-labeled antisense phosphorothioate DNA targeting the mdr1 mRNA improves after electrostatic complexation with the transmembrane transfector (TF) carriers Neophectin™ or jetPEI™ as was observed by us in vitro.MethodsThe biodistribution of the labeled antisense DNA before and after complexation with either TF was determined in nude mice bearing KB-G2 (Pgp++) tumors.ResultsComplexation with either TF resulted in significantly higher background radioactivity levels in almost all normal tissues and modest improvement in tumor accumulation at best. The tumor accumulation was lower compared to naked at six hours (0.34 and 0.23 vs. 0.40% ID/g) and modestly higher at 24–28 hours (0.15 and 0.15 vs. 0.12% ID/g) for Neophectin and jetPEI, respectively. That blood was less than 0.18% ID/g for both TFs even at six hours suggests that tumor accumulations may have suffered from rapid blood clearance.ConclusionThe results of this investigation show that because of the unfavorable pharmacokinetics of radiolabeled phosphorothioate DNAs when electrostatically complexed to jetPEI or Neophectin, neither TF appears to be useful in vivo despite favorable results in vitro. Future studies will devote greater consideration to the relative rates of tumor accumulation and blood clearance.
Bioconjugate Chemistry | 2004
Kayoko Nakamura; Fan C; Guozheng Liu; Jiang He; Shuping Dou; Atsushi Kubo; Mary Rusckowski; Donald Hnatowich
Society of Nuclear Medicine Annual Meeting Abstracts | 2008
Ling Chen; Yi Wang; Xinrong Liu; Guozheng Liu; Shuping Dou; Nan Xiao; Donald Hnatowich; Mary Rusckowski
Society of Nuclear Medicine Annual Meeting Abstracts | 2012
Yuzhen Wang; Ling Chen; Xinrong Liu; Guozheng Liu; Shuping Dou; Donald Hnatowich; Mary Rusckowski
Society of Nuclear Medicine Annual Meeting Abstracts | 2012
Guozheng Liu; Shuping Dou; Miles Smith; Yuzhen Wang; Mary Rusckowski; Donald Hnatowich
Society of Nuclear Medicine Annual Meeting Abstracts | 2011
Guozheng Liu; Shuping Dou; Mary Rusckowski; Philip R. Streeter; Dale Greiner; Donald Hnatowich
Society of Nuclear Medicine Annual Meeting Abstracts | 2011
Guozheng Liu; Shuping Dou; Yuxia Liu; Yuzhen Wang; Mary Rusckowski; Donald Hnatowich
Society of Nuclear Medicine Annual Meeting Abstracts | 2010
Ling Chen; Dengfeng Cheng; Yi Wang; Guozheng Liu; Shuping Dou; Xinrong Liu; Donald Hnatowich; Mary Rusckowski
Society of Nuclear Medicine Annual Meeting Abstracts | 2010
Nan Xiao; Xinrong Liu; Dengfeng Cheng; Yuxia Liu; Guozheng Liu; Ling Chen; Minmin Liang; Donald Hnatowich; Mary Rusckowski
Society of Nuclear Medicine Annual Meeting Abstracts | 2010
Xinrong Liu; Kayoko Nakamura; Dengfeng Cheng; Minmin Liang; Yuxia Liu; Ling Chen; Mary Rusckowski; Donald Hnatowich