E.F. Soares

Effect of Composite Containing an Iodonium Salt on the Bond Strength of Brackets to Bovine Enamel

This study investigated the effect of the incorporation of an iodonium salt in experimental composites, on the bond strength of metallic brackets bonded to bovine teeth. Two hundred and seventy bovine teeth were embedded in self-curing acrylic resin and divided into 18 groups (n=15), according to the experimental composite with an iodonium salt at molar concentrations 0 (control), 0.5, or 1%; the light-activation times (8, 20 and 40 s); and the storage times (10 min or 24 h). Metallic brackets were fixed on the tooth surface using experimental composites. Photoactivation was performed with a quartz-tungsten-halogen light-curing unit curing unit for 8, 20 and 40 s. The specimens were stored in distilled water at 37 °C for 10 min or 24 h and submitted to bond strength test at 0.5 mm/min. The data were subjected to three-way ANOVA and Tukeys test (α=0.05). The Adhesive Remnant Index (ARI) was used to classify the failure modes. The shear bond strengths (MPa) at 10 min for light-activation times of 8, 20 and 40 s were: G1 - 4.6, 6.9 and 7.1; G2 - 8.1, 9.2 and 9.9; G3 - 9.1, 10.4 and 10.7; and at 24 h were: G1 - 10.9, 11.1 and 11.7; G2 - 11.8, 12.7 and 14.2; G3 - 12.1, 14.4 and 15.8. There was a predominance of ARI score 3 for groups with 10 min storage time, and ARI score 2 for groups with 24 h storage time. In conclusion, the addition of iodonium salt (C05 and C1) to the experimental composite may increase the bond strength of brackets to bovine enamel using reduced light exposure times.

A new arginine-based dental adhesive system: formulation, mechanical and anti-caries properties

Secondary caries at the margins of composite restorations has been attributed to adhesive failure and consequent accumulation of cariogenic biofilms. OBJECTIVES To develop and evaluate an etch-and-rinse adhesive system containing arginine for sustainable release and recharge without affecting its mechanical properties. Arginine metabolism by oral bacteria generates ammonia, which neutralizes glycolytic acids and creates a neutral environmental pH that is less favorable to the growth of caries pathogens, thus reducing the caries risk at the tooth-composite interface. METHODS Experimental adhesives were formulated with methacrylate monomers and arginine at 5%, 7%, and 10% or no arginine (control). Adhesives were tested for: (i) mechanical properties of true stress (FS and UTS), modulus of elasticity (E), degree of conversion (DC), Knoop hardness number (KHN) and dentin microtensile bond strength (μ-TBS), (ii) arginine release and recharge, and (iii) antibacterial activities. Data was analyzed by t-test, one-way ANOVA and Tukeys tests. RESULTS FS and UTS results showed no statistically significant differences between the 7% arginine-adhesive and control, while the results for E, DC, KHN and μ-TBS showed no difference among all groups. The 7% arginine-adhesive showed a high release rate of arginine (75.0μmol/cm2) at 2h, and a more sustainable, controlled release rate (up to 0.2μmol/cm2) at 30days. CONCLUSIONS Incorporation of 7% arginine did not affect the physical and mechanical properties of the adhesive. Arginine was released from the adhesive at a rate and concentration that exhibited antibacterial effects, regardless of shifts in biofilm conditions such as sugar availability and pH. CLINICAL SIGNIFICANCE Secondary caries is recognized as the main reason for failure of dental restorations. The development of an arginine-based adhesive system has the potential to dramatically reduce the incidence and severity of secondary caries in adhesive restorations in a very economical fashion.

Influence of Different Concentration and Ratio of a Photoinitiator System on the Properties of Experimental Resin Composites

The aim in this study was to evaluate the influence of different ratio of camphorquinone/tertiary amine concentration on the flexural strength (FS), elastic modulus (EM), degree of conversion (DC), yellowing (YL), water sorption (WS) and water solubility (WSL) of experimental composites. Thus, acrylate blends were prepared with different camphorquinone (CQ) and amine (DABE) concentrations and ratios by weight: (CQ/DABE%): 0.4/0.4% (C1), 0.4/0.8% (C2), 0.6/0.6% (C3), 0.6/1.2% (C4), 0.8/0.8% (C5), 0.8/1.6% (C6), 1.0/1.0% (C7), 1.0/2.0% (C8), 1.5/1.5% (C9), 1.5/3.0% (C10). For the FS and EM, rectangular specimens (7x2x1 mm, n=10) were photo-activated by single-peak LED for 20 s and tested at Instron (0.5 mm/min). Then, the same specimens were evaluated by FTIR for DC measurement. For YL, disks (5x2 mm, n=10) were prepared, light-cured for 20 s and evaluated in spectrophotometer using the b aspect of the CIEL*a*b* system. For WS and WSL, the volume of the samples was calculated (mm³). For WS and WSL, composites disks (5x0.5 mm, n=5) were prepared. After desiccation, the specimens were stored in distilled water for 7 days and again desiccated, in order to measure the WS and WSL. Data were submitted to one-way ANOVA and Tukeys test (5%). The groups C8, C9 and C10 showed higher DC, EM and YL means, compared to other composites. Therefore, the FS and WS values were similar among all groups. Also, C1, C2 and C3 presented higher WSL in 7 days, compared to other composites. In general, higher concentrations of camphorquinone promoted higher physical-mechanical properties; however, inducing higher yellowing effect for the experimental composites.

Microtensile Bond Strength of Adhesive Systems in Different Dentin Regions on a Class II Cavity Configuration

The aim of this study was to evaluate microtensile bond strength (µTBS) of self-etch and etch-and-rinse adhesives systems compared in different dentin regions (central-CD or proximal-PD) in a class II cavity configuration. A class II (mesial-oclusal-distal) cavity configuration was simulated on 20 extracted human third-molars (4 mm wide/3 mm deep). Etch-and-rinse adhesive (Scotchbond Multi Purpose, n=5, SBMP and Optibond FL, n=5, OPFL) and self-etch adhesives (Clearfil SE Bond, n=5, CSE and Optibond XTR, n=5, OPXTR) were applied. Class II restorations were performed by incremental technique and photo-activated (Bluephase/G2). Samples were sectioned to beam shape (1 mm² cross-section), placed on Geraldelis device for µTBS test (0.5 mm/min cross-head speed). Fracture patterns were analyzed on stereomicroscope and classified as cohesive-resin, adhesive, mixed/resin or mixed/dentin. Samples (n=4) were prepared for scanning electron microscope observation. Data were submitted to one-way ANOVA with Split-Plot arrangement and Tukeys test (α=0.05). There were no statistically significant differences among SBMP, OPFL, CSE and OPXTR on CD (p>0.05). However, on PD for SBMP and OPFL, µTBS values were significantly lower compared to CSE and OPXTR (p<0.05). In all groups, mixed failure pattern was more frequently observed, except for SBMP/CD (adhesive). In class II type cavity configuration, PD location negatively influenced bond strength of etch-and-rinse adhesive systems. Opposite to self-etching adhesives, which presented higher bond strength values compared to etch-and-rinse adhesives in PD.

EFEITO DO USO DE SISTEMAS ADESIVOS, ARMAZENAGEM E CLORIDRATO DE DOXICICLINA NA RESISTÊNCIA DE UNIÃO À MICROTRAÇÃO NA DENTINA

Resumo Este estudo avaliou a influência da aplicação do cloridrato de doxiciclina (DOX) nas concentrações de 3% e 10% na dentina humana através da resistência de união à microtração (RUμT), após 24 horas e 6 meses. A superfície oclusal de 60 terceiros molares humanos foi cortada, desgastada com lixas de granulação 600 e divididas em 6 grupos (n=10): 1 e 4 – Controle (sem aplicação da DOX); 2 e 5 – aplicação da concentração de DOX 3%; e, 3 e 6 – aplicação da concentração de DOX 10%. A superfície da dentina foi condicionada com ácido fosfórico a 35% por 15 s, lavado e seco. Em seguida, foi aplicada sobre a dentina a DOX 3% e 10%, por 5 minutos, o excesso foi removido deixando a dentina úmida e aplicado o adesivo Adper Scotchbond Multi-Purpose. Um bloco da resina composta Filtek Z250 foi construído sobre a superfície da dentina em três incrementos até atingir a altura de 5mm, sendo que cada incremento fotoativado por 20 s com o aparelho LEDBluephase. Todas as amostras foram armazenadas em água deionizada a 37o C por 24 horas e seccionadas perpendicularmente à área de união, de modo a obter palitos com secção transversal de 1 mm2. Os palitos dos grupos 1 a 3 foram submetidos ao ensaio de RUμT à velocidade de 1,0 mm/min., até ocorrer a fratura após 24 horas e os palitos dos grupos 4 a 6, após 6 meses. Os dados foram submetidos à Análise de Variância dois fatores e ao teste de Tukey (α = 0,05). Os valores de RUμT (MPa) da doxiciclina 10% 46,7±2,1 foi significativamente superior ao grupo controle 38,3±2,8 (p<0,05). Nenhuma diferença estatística foi observada entre a DOX 10% e 3% 42,4±2,4, DOX 3% e grupo controle (p>0,05). Os valores de RUμT para 24 horas 27,4±1,9 foi estatisticamente superior a 6 meses 23,1±2,5 (p<0,05). O uso da DOX 10% aumentou significativamente a RUμT e a armazenagem reduziu a RUμT. .