E.G. Gregorich

Soil Sampling and Methods of Analysis

SOIL SAMPLING AND HANDLING, G.T. Patterson and M.R. Carter Soil Sampling Designs, D. Pennock, T. Yates, and J. Braidek Sampling Forest Soils, N. Belanger and K.C.J. Van Rees Measuring Change in Soil Organic Carbon Storage, B.H. Ellert, H.H. Janzen, A.J. VandenBygaart, and E. Bremer Soil Sample Handling and Storage, S.C. Sheppard and J.A. Addison Quality Control in Soil Chemical Analysis, C. Swyngedouw and R. Lessard DIAGNOSTIC METHODS for SOIL and ENVIRONMENTAL MANAGEMENT, J.J. Schoenau and I.P. OHalloran Nitrate and Exchangeable Ammonium Nitrogen, D.G. Maynard, Y.P. Kalra, and J.A. Crumbaugh Mehlich 3 Extractable Elements, N. Ziadi and T. Sen Tran Sodium Bicarbonate Extractable Phosphorus, J.J. Schoenau and I. P. OHalloran Boron, Molybdenum and Selenium, G. M. Hettiarachchi and U. C. Gupta Trace Element Assessment, W.H. Hendershot, H. Lalande, D. Reyes, and D. MacDonald Readily Soluble Aluminum and Manganese in Acid Soils, Y.K. Soon, N. Belanger, and W.H. Hendershot Lime Requirement, N. Ziadi and T. Sen Tran Ion Supply Rates Using Ion Exchange Resins, P. Qian, J.J. Schoenau, and N. Ziadi Environmental Soil Phosphorus Indices, A.N. Sharpley, P.J.A. Kleinman and J.L. Weld Electrical Conductivity and Soluble Ions, J.J. Miller and D. Curtin SOIL CHEMICAL ANALYSES, Y.K. Soon and W.H. Hendershot Soil Reaction and Exchangeable Acidity, W.H. Hendershot, H. Laland,e and M. Duquette Collection and Characterization of Soil Solutions, J.D. MacDonald, N. Belanger, S. Sauve, F. Courchesne, and W.H. Hendershot Ion Exchange and Exchangeable Cations, W.H. Hendershot, H. Lalande, and M. Duquette Non-Exchangeable Ammonium, Y.K. Soon and B.C. Liang Carbonates, T.B. Goh and A.R. Mermut Total and Organic Carbon, J.O. Skjemstad and J.A. Baldock Total Nitrogen, P.M. Rutherford, W.B. McGill, C.T. Figueiredo, and J.M. Arocena Chemical Characterization of Soil Sulphur, C.G. Kowalenko and M. Grimmett Total and Organic Phosphorus, I.P. OHalloran and B.J. Cade-Menum Characterization of Available P by Sequential Extraction, H. Tiessen and J.O. Moir Extractable Al, Fe, Mn, and Si, F. Courchesne and M.C. Turmel Determining Nutrient Availability in Forest Soils, N. Belanger, David Pare, and W.H. Hendershot Chemical Properties of Organic Soils, A. Karam SOIL BIOLOGICAL ANALYSES, E. Topp and C.A. Fox Cultural Methods for Soil and Root Associated Microorganisms, J.J. Germida and J.R. de Freitas Arbuscular Mycorrhiza, Y. Dalpe and C. Hamel Root Nodule Bacteria and Symbiotic Nitrogen Fixation, D. Prevost and H. Antoun Microarthropods, J.P Winter and V.M. Behan-Pelletier Nematodes, T.A. Forge and J. Kimpinski Earthworms, M.J. Clapperton, G.H. Baker and C.A. Fox Enchytraeids, S.M. Adl Protozoa, S.M. Adl, D. Acosta-Mercado, and D.H. Lynn Denitrification Techniques for Soils, C.F. Drury, D.D. Myrold, E.G. Beauchamp, and W.D.Reynolds Nitrification Techniques in Soil Systems, C.F. Drury, S.C. Hart, and X.M. Yang Substrate-Induced Respiration and Selective Inhibition as Measures of Microbial Biomass in Soils, V.L. Bailey, J.L. Smith, and H. Bolton Jr. Assessment of Soil Biological Activity, R.P.Beyaert and C.A. Fox Soil ATP, R.P. Voroney, G. Wen, and R.P. Beyaert Lipid-Based Community Analysis, K.E. Dunfield Bacterial Community Analyses by Denaturing Gradient Gel Electrophoresis (DGGE), E. Topp, Y.-C. Tien, and A. Hartmann Indicators of Soil Food Web Properties, T.A. Forge and M. Tenuta SOIL ORGANIC MATTER ANALYSES, E.G. Gregorich and M.H. Beare Carbon Mineralization, D.W. Hopkins Mineralizable Nitrogen, Denis Curtin and C.A. Campbell Physically Uncomplexed Organic Matter, E.G. Gregorich and M.H. Beare Extraction and Characterization of Dissolved Organic Matter, M.H. Chantigny, D.A. Angers, K. Kaiser, and K. Kalbitz Soil Microbial Biomass C, N, P and S, R.P. Voroney, P.C. Brookes, and R.P. Beyaert Carbohydrates, M.H. Chantigny and D.A. Angers Organic Forms of Nitrogen, D.C. Olk Soil Humus Fractions, D.W. Anderson and J.J Schoenau Soil Organic Matter Analysis by Solid-State 13C Nuclear Magnetic Resonance Spectroscopy, M. J. Simpson and C. M. Preston Stable Isotopes in Soil and Environmental Research, B.H. Ellert and L. Rock SOIL PHYSICAL ANALYSES, D.A. Angers and F.J. Larney Particle Size Distribution, D. Kroetsch and C. Wang Soil Shrinkage, C.D. Grant Soil Density and Porosity, X. Hao, B.C. Ball, J.L.B. Culley, M.R. Carter, and G.W. Parkin Soil Consistency: Upper and Lower Plastic Limits, R.A. McBride Compaction and Compressibility, P. Defossez, T. Keller and G. Richard Field Soil Strength, G.C. Topp and D.R. Lapen Air Permeability, C.D. Grant and P.H. Groenevelt Aggregate Stability to Water, D.A. Angers, M.S. Bullock, and G.R. Mehuys Dry Aggregate Size Distribution, F.J. Larney Soil Air, R.E. Farrell and J.A. Elliott Soil-Surface Gas Emissions, P. Rochette and N. Bertrand Bulk Density Measurement in Forest Soils, D.G. Maynard and M.P. Curran Physical Properties of Organic Soils and Growing Media: Particle Size and Degree of Decomposition, L.E. Parent and J. Caron Physical Properties of Organic Soils and Growing Media: Water and Air Storage and Flow Dynamics, J. Caron, D.E. Elrick, J.C. Michel, and R. Naasz SOIL WATER ANALYSES, W.D. Reynolds and G.C. Topp Soil Water Analyses: Principles and Parameters, W.D. Reynolds and G.C. Topp Soil Water Content, G.C. Topp, G.W. Parkin, and Ty P.A Ferre Soil Water Potential, N.J. Livingston and G.C. Topp Soil Water Desorption and Imbibition: Tension and Pressure Techniques, W.D. Reynolds and G.C. Topp Soil Water Desorption and Imbibition: Long Column, W.D. Reynolds and G.C. Topp Soil Water Desorption and Imbibition: Psychrometry, W.D. Reynolds and G.C. Topp Saturated Hydraulic Properties: Laboratory Methods, W.D. Reynolds Saturated Hydraulic Properties: Well Permeameter, W.D. Reynolds Saturated Hydraulic Properties: Ring Infiltrometer, W.D. Reynolds Saturated Hydraulic Properties: Auger-Hole, G.C. Topp Saturated Hydraulic Properties: Piezometer, G.C. Topp Unsaturated Hydraulic Properties: Laboratory Tension Infiltrometer, F.J. Cook Unsaturated Hydraulic Properties: Laboratory Evaporation, O.O. B. Wendroth and N. Wypler Unsaturated Hydraulic Properties: Field Tension Infiltrometer, W.D. Reynolds Unsaturated Hydraulic Properties: Instantaneous Profile, W.D. Reynolds Estimation of Soil Hydraulic Properties, F.J. Cook and H.P. Cresswell Analysis of Soil Variability, B.C. Si, R.G. Kachanoski, and W.D. Reynolds APPENDIX Site Description, G.T. Patterson and J.A. Brierley General Safe Laboratory Operation Procedures, P. St-Georges INDEX

Impact of tillage practices on organic carbon and nitrogen storage in cool, humid soils of eastern Canada

Abstract Soil organic matter storage capacity in agroecosystems varies with soil type, climate and agricultural management practices. The effects of different tillage systems on organic C and N storage were determined for a range of soils of eastern Canada mainly under continuous corn and small grain cereal production. Soil profiles from eight sites on which comparative tillage experiments had been performed for up to 11 years were sampled to a 60 cm depth in four increments (0–10, 10–20, 20–40 and 40–60cm). Organic C and N contents and dry bulk density were determined for each sampling depth. Bulk density measurements showed that the total soil mass in the soil profiles was not influenced by the tillage systems. No significant differences were found between tillage treatments in the total organic C and N storage down to 60 cm depth; the soil profiles under no-till (NT) and chisel plowing (CP) generally did not contain more C and N than those under conventional moldboard plowing (MP). However, the depth distribution of soil C and N varied with tillage. In the surface 0–10cm, C and N contents were higher under NT than under MP, whereas at deeper levels (20–40cm) the reverse trend was observed. It is concluded than under eastern Canadian conditions, where crop production and residue inputs are not affected by tillage, reduced tillage systems would not result in the storage of more soil organic matter in the entire soil profile at least in a 5–10 year period. Placement of the residues would be a major factor influencing the C and N distribution at specific depths.

Influence of agricultural management on soil organic carbon: A compendium and assessment of Canadian studies

To fulfill commitments under the Kyoto Protocol, Canada is required to provide verifiable estimates and uncertainties for soil organic carbon (SOC) stocks, and for changes in those stocks over time. Estimates and uncertainties for agricultural soils can be derived from long-term studies that have measured differences in SOC between different management practices. We compiled published data from long-term studies in Canada to assess the effect of agricultural management on SOC. A total of 62 studies were compiled, in which the difference in SOC was determined for conversion from native land to cropland, and for different tillage, crop rotation and fertilizer management practices. There was a loss of 24 ± 6% of the SOC after native land was converted to agricultural land. No-till (NT) increased the storage of SOC in western Canada by 2.9 ± 1.3 Mg ha-1; however, in eastern Canada conversion to NT did not increase SOC. In general, the potential to store SOC when NT was adopted decreased with increasing backgr...

Biodegradability of soluble organic matter in maize-cropped soils

Abstract Soluble organic matter and its biodegradability are important in relation to soil nutrient fluxes, carbon (C) sequestration, and water quality. The objective of this study was to evaluate the quantity and biodegradability of soluble organic matter in soil under monoculture maize or maize−soybean rotation, and different amendments: manure, inorganic fertilizer, or no amendment. We characterized organic matter extracted in cold and hot (80 °C) water by using a bioassay involving incubation of the extracts (after inoculation with soil microflora) at 35 °C for 42 days. Soluble organic C and organic and mineral nitrogen (N) were monitored during the incubation. Extracts of whole soil (0–15 cm depth) and three water-stable aggregate size classes (>1000, 1000–250, and 250–50 μm) were subjected to the bioassay in order to evaluate the physical disposition and kinetics of soluble organic matter. Hot water-soluble C accounted for about 70% of the total (cold+hot) water-soluble organic matter. Organic N was the major form of N in the extracts; it comprised 61–83% of the total N extracted with cold water and 87–97% of the total N extracted with hot water. The quantity of soluble organic matter that was biodegradable was related to the extraction procedure and the management history of the soil. The proportion of soluble C and organic N that was biodegradable was greatest in hot water extracts and greater in manured than nonmanured soils. During the bioassay, proportionately greater amounts of organic N were metabolized relative to organic C, so that the C:N ratios of the extracts widened as the incubation progressed, indicating that the soluble organic matter was rich in labile organic N. The rate at which soluble organic matter decomposed (i.e., turnover) was not related to the type of input. Decomposition data fitted a double exponential decay model, suggesting that the soluble organic matter comprised two fractions: a rapidly decomposable fraction (containing 29–36% of the total soluble C) with a turnover time of The concentration of soluble organic matter in soil aggregates was related to aggregate size; the concentration was greatest in the largest aggregates and it decreased with decreasing aggregate size. Soluble organic C extracted from aggregates also comprised two kinetically discrete pools. The turnover time (i.e., mean residence time) of each pool was not affected by amendment, crop rotation, or aggregate size. However, the slowly decomposable organic C pool was larger in microaggregates (

Organic C and N storage, and organic C fractions, in adjacent cultivated and forested soils of eastern Canada

As a major attribute of soil quality, organic matter is responsive to agricultural land use practices including tillage. A study was initiated in eastern Canada to characterize changes in the masses of organic C and total N, and organic matter fractions in forested and adjacent cultivated or forage sites. Generally, the cultivated and forage sites had denser soil profiles than the forest sites. Based on an equivalent soil mass, to accommodate differences in soil bulk density, the paired forest and cultivated sites showed that cultivation decreased the mass of organic C (35%) and total N (10%) in the soil profile of the Podzolic soils, but increased organic C (25%) and total N (37%) in the Brunisolic (Cambisol) and Gleysolic soils. For the Podzolic soils, use of forages increased soil stored organic C and N by 55% and 35%, respectively. Organic C fractions were mainly of significance in the A horizon. Soil microbial biomass C was greater in the forested, compared to the cultivated soil, but the proportion of soil organic C as microbial biomass C (1.3% to 1.6%) was similar. The proportion, however, was greater (2.1%) for the forage soil, compared to the corresponding cultivated (1.3%) soil, suggesting that organic C was continuing to increase under the former. The relatively large proportion (19%) of organic C found in the light fraction of forest soils in the A horizon was decreased (up to 70%) by cultivation. In contrast, the proportion of macro-organic C present in the soil sand fraction was not greatly influenced by cultivation. Overall, soils in eastern Canada have a relatively large potential to store organic matter. The study illustrates the importance of soil type and cultivation interactions for maintenance of soil organic matter storage, and the positive influence of forages in this regard in agroecosystems.

Controls on the distribution of productivity and organic resources in Antarctic Dry Valley soils

The Antarctic Dry Valleys are regarded as one of the harshest terrestrial habitats on Earth because of the extremely cold and dry conditions. Despite the extreme environment and scarcity of conspicuous primary producers, the soils contain organic carbon and heterotrophic micro-organisms and invertebrates. Potential sources of organic compounds to sustain soil organisms include in situ primary production by micro-organisms and mosses, spatial subsidies from lacustrine and marine-derived detritus, and temporal subsidies (‘legacies’) from ancient lake deposits. The contributions from these sources at different sites are likely to be influenced by local environmental conditions, especially soil moisture content, position in the landscape in relation to lake level oscillations and legacies from previous geomorphic processes. Here we review the abiotic factors that influence biological activity in Dry Valley soils and present a conceptual model that summarizes mechanisms leading to organic resources therein.

Characterisation of water transmission properties in tilled and untilled soils using tension infiltrometers

Tension infiltrometer (TI) measurements from a silty clay loam soil (Winchester, Ont.), a sandy soil (Hancock, WI), and a silt loam soil (Rosemount, MN) were used to: (i) characterise near-saturated hydraulic conductivity (K0) and flow-weighted mean radius of soil macropores (R0); (ii) distinguish differences in these water transmission properties between no-till (NT) and mouldboard plough (MP) continuous maize (Zea mays L.) production systems. n nThe K0 values increased by about two orders of magnitude as the pressure heads (P0) set on the TI membranes were increased incrementally from the minimum values (P0=−10 cm or −15 cm) to the maximum value (P0=0 cm). This indicates that substantial networks of water-conducting soil macropores exist in continuous maize production systems, regardless of soil texture or tillage treatment. For each P0 value, the MP treatment had a consistently higher K0 than NT at the Winchester and Hancock fields sites, and a consistently lower K0 than NT at the Rosemount field site. n nRegardless of soil type, most R0 pores occurred in the 0.1–0.3 mm size range for both NT and MP soils, but NT had two to three times more of these R0 pore sizes, as well as smaller and larger R0 pores, than MP. This probably reflects a more consolidated soil matrix (enrichment of smaller R0 pores) and a greater number of large cracks and biopores (larger R0 pores) in NT soils, owing to the absence of annual loosening of the soil matrix and disruption of macropores that occurs with MP tillage. n nRelationships between K0 and R0 were complex but consistent within and between tillage treatments. A physical interpretation for this behaviour is given which employs capillary theory for water entry, and interactions among the size, number and morphology of water-conducting macropores during the infiltration process. n nIt was concluded that the TI technique is effective for characterising differences in K0 and R0 between NT and MP continuous maize production systems.

Changes in soil physical properties with depth in a conventionally tilled soil after no-tillage

A no-tillage system was imposed on a structurally degraded fine-textured soil (Humic Gleysol) that had been under continuous corn with moldboard tillage for more than 20 years. After 3 years of no-tillage, several soil structural properties were compared with the conventional tillage treatment to assess whether the soil structure had improved. n nNo significant difference (P<0.05) was found between tillage treatments for the saturated hydraulic conductivity, porosity and penetration resistance in the surface 5 cm. Measurements of soil penetration resistance and in situ saturated hydraulic conductivity (Kwp) using the well permeameter method were sensitive to structural changes that had occurred at 5–20 cm depth. The Kwp at this depth was significantly greater in the moldboard treatment than in the no-tillage treatment. Resistance measurements indicated significantly greater soil strengths at 10–20 cm under no-tillage. Aggregate stabilities were assessed by wet sieving twice during the growing season. No-tillage resulted in larger soil aggregates, especially at the surface, compared with the moldboard tillage. n nThese data suggest that degraded soils with low structural stability may initially suffer further deterioration with the elimination of tillage, owing to the loss or reduction of mechanically formed pores.

Decomposition of residues and loss of the δ-endotoxin from transgenic (Bt) corn (Zea mays L.) in soil

Corn and other crops genetically modified to express the insecticidal δ-endotoxin from Bacillus thuringiensis (Bt) are grown widely across north America. Studies have shown that the δ -endotoxin can be stabilised on soil colloids where its activity is retained, but reports of direct ecological effects of the δ-endotoxin on soil processes are limited. We have determined the concentrations of the δ-endotoxin in organic residues fro m Bt-corn plants at increasing stages of ageing and decay, and the subsequent decomposition in soil of these residues and the δ-endotoxin in them. The δ-endotoxin concentrations declined from 6.8 μg g-1 in the fresh plant material, to 0.82 μg g-1 in the post-harvest residues collected in the fall, and to 0.026 μg g-1 in the residues collected from soil surface the following spring. The concentration of δ -endotoxin in buried residues collected in the spring was not significantly different from zero. When incubated in soil in the laboratory over 84 d, the δ-endotoxin decomposed mo...