E. Patrick Fuerst

Effect of tillage, fungicide seed treatment, and soil fumigation on seed bank dynamics of wild oat (Avena fatua)

Abstract No-tillage offers potential for improved soil quality, reduced erosion, and equal or increased crop yields. We hypothesized that, compared with conservation tillage (CT), no-tillage (NT) offers conditions more conducive to microbial decay of weed seed. In NT systems seed remain at or near the soil surface where crop residues, moisture, and lack of disturbance create an environment with greater soil microbial diversity. In late fall of 1998 and 1999, dormant seed of wild oat, either individually glued to plastic toothpicks or mixed with soil and placed in mesh bags, were buried (mean seed depth of 2.5 cm) in replicated field plots managed by NT or CT since 1982. Treatments including fungicide seed treatment (thiram + metalaxyl + captan) and soil fumigation (propylene oxide) provided estimates of the contribution of microorganisms to observed mortality. Seed were retrieved in May and August, 1999 and 2000. Contrary to our original hypothesis, the proportion of dead seed was generally similar in NT and CT systems. Lack of tillage system by seed or soil treatments affecting the proportion of dead or decayed seed suggests that the contribution of microorganisms to seed fate is similar in these tillage environments. However, the proportion of dormant seed was consistently lower in the NT compared with CT treatments; there was a corresponding increase in the proportion of germinated seed. Overall, more than half of the wild oat seed bank losses could be directly attributed to germination whereas losses due to decay were relatively minor by comparison. Despite favorable distribution of seed and improved quality of the surface-strata of soil in NT systems, this study fails to provide evidence that enhanced microbial decay will contribute to a “weed-suppressive” capacity in such cropping systems. Nomenclature: Captan, N-trichloromethylthio-4-cyclohexene-1,2-dicarboximide, metalaxyl, N-(2,6-dimethylphenyl)-N-(methoxyacetyl)alanine methyl ester; propylene oxide; thiram, tetramethylthiuram disulfide; wild oat, Avena fatua L. AVEFA; wheat, Triticum aestivum L. ‘Madsen’.

Polyphenol Oxidase in Wheat Grain: Whole Kernel and Bran Assays for Total and Soluble Activity

ABSTRACT Color is a key quality trait of wheat products, and polyphenol oxidase (PPO) is implicated as playing a significant role in darkening and discoloration. In this study, total and soluble PPO activities were characterized in whole kernel assays and bran extracts. In whole kernel assays similar to AACC Approved Method 22–85, four wheat cultivars were ranked the same for both total and soluble (leached) PPO activity with L-DOPA (diphenol) as the substrate. Total kernel PPO activity was much greater than soluble PPO activity in three hexaploid wheat cultivars, indicating that insoluble PPO was the major contributor to kernel PPO measurements. Tyrosine (monophenol) was an excellent PPO substrate in kernel assays as expected but had no activity as a substrate for soluble PPO. However, soluble PPO activity with tyrosine was activated by the addition of the diphenols chlorogenic acid and caffeic acid. When PPO was assayed in homogenized bran, 89–95% of total PPO activity remained insoluble, associated wit...

Effect of processing on phenolic composition of dough and bread fractions made from refined and whole wheat flour of three wheat varieties.

This study investigated the effect of breadmaking on the assay of phenolic acids from flour, dough, and bread fractions of three whole and refined wheat varieties. Comparison of the efficacy of two commonly used methods for hydrolysis and extraction of phenoilc acids showed that yields of total phenolic acids (TPA) were 5-17% higher among all varieties and flour types when samples were directly hydrolyzed in the presence of ascorbate and EDTA as compared to the method separating free, soluble conjugates and bound, insoluble phenolic acids. Ferulic acid (FA) was the predominant phenolic acid, accounting for means of 59 and 81% of TPA among all refined and whole wheat fractions, respectively. All phenolic acids measured were more abundant in whole wheat than in refined samples. Results indicated that the total quantified phenolic acids did not change significantly when breads were prepared from refined and whole wheat flour. Thus, the potential phytochemical health benefits of total phenolic acids appear to be preserved during bread baking.

Polyphenol oxidase as a biochemical seed defense mechanism

Seed dormancy and resistance to decay are fundamental survival strategies, which allow a population of seeds to germinate over long periods of time. Seeds have physical, chemical, and biological defense mechanisms that protect their food reserves from decay-inducing organisms and herbivores. Here, we hypothesize that seeds also possess enzyme-based biochemical defenses, based on induction of the plant defense enzyme, polyphenol oxidase (PPO), when wild oat (Avena fatua L.) caryopses and seeds were challenged with seed-decaying Fusarium fungi. These studies suggest that dormant seeds are capable of mounting a defense response to pathogens. The pathogen-induced PPO activity from wild oat was attributed to a soluble isoform of the enzyme that appeared to result, at least in part, from proteolytic activation of a latent PPO isoform. PPO activity was also induced in wild oat hulls (lemma and palea), non-living tissues that cover and protect the caryopsis. These results are consistent with the hypothesis that seeds possess inducible enzyme-based biochemical defenses arrayed on the exterior of seeds and these defenses represent a fundamental mechanism of seed survival and longevity in the soil. Enzyme-based biochemical defenses may have broader implications since they may apply to other defense enzymes as well as to a diversity of plant species and ecosystems.

Modeling site-specific wild oat (Avena fatua) emergence across a variable landscape

Abstract The spatial and temporal pattern of wild oat emergence in eastern Washington is affected by the steep, rolling hills that dominate this landscape. The objective of this study was to assess the impact of landscape position and crop residue on the emergence phenology of wild oat. Emergence of a natural wild oat infestation was characterized over two growing seasons (2003 and 2004), at two wheat residue levels (0 and 500 g m−2), and at five landscape positions differing in slope, aspect, and elevation in a no-till winter wheat field. Wild oat emerged 1 to 2 wk earlier at south-facing landscape positions than at north-facing landscape positions. Crop residue delayed wild oat emergence by 7 to 13 d relative to bare soil at south-facing positions in 2003 and had a reduced effect on emergence at north-facing landscape positions. Therefore, preserving surface residues tended to synchronize emergence across the landscape and may facilitate better timing of weed control where residue is present. Emergence of wild oat was modeled as a function of thermal time adjusted by water potential using a Weibull function. Temperature explained more variation in the model than water potential. This model explained much of the variability in wild oat emergence among landscape positions over these 2 yr and may be useful as a tool to predict the timing of wild oat emergence. Results also indicate that site-specific modeling is a plausible approach to improving prediction of weed seedling emergence. Nomenclature: Wild oat, Avena fatua L., AVEFA; winter wheat, Triticum aestivum L.

Wheat Arabinoxylan Structure Provides Insight into Function

ABSTRACT Recent attention to dietary fiber in wheat (Triticum aestivum L.) has invigorated research in the nonstarch carbohydrate arabinoxylan (AX). AX molecules are composed of a linear xylose backbone with arabinose substitutions along the backbone. These arabinose substituents can also carry a ferulic acid moiety. AX molecules can be fractionated into two categories based on extraction properties that have a structural and conformational basis: water-extractable (WEAX) and water-unextractable (WUAX) molecules. The ferulic acid moieties also allow for oxidative cross-linking between AX molecules or the tyrosine residues of proteins. The contents of total AX and WEAX molecules are primarily influenced by genetic differences; however, there is also evidence of environmental influence on content. There are several useful methods for quantifying AX molecules, providing varying levels of structural information as well as accuracy and precision. The high water-absorption capacity of AX molecules results in a ...

Some observations on the granivorous feeding behavior preferences of the house mouse (Mus musculus L.)

Abstract The house mouse (Mus musculus) is a highly successful mammal worldwide, in part due to its adaptive consumption of a wide range of seeds, especially those of the agricultural cereal crops. The present study examined the granivorous feeding behavior of mice in relation to wheat kernel texture (hardness), bran color, and the presence/absence of hulls. Three wheat taxa were examined: common bread wheat, spelt, and emmer. There was a particularly strong (5-fold) preference for soft white wheat kernels over hard red kernels. Using near-isogenic wheat lines, the preference was most highly associated with softer texture. This preference was also evidenced in spelt (soft) vs. emmer (very hard) comparisons. For spelt, hulls presented no deterrent to consumption, whereas in emmer, the hulls significantly reduced consumption. In a number of trials, a short lag in consumption patterns of new foods suggested that learning was involved. Regardless of the factor evaluated, all grains were consumed at some level. Soft white wheat was preferred over laboratory pellets by about 4 to 1. The results raise further questions about the ways mice evaluate individual grains and make consumption choices.

Half-Embryo Cocultivation Technique for Estimating the Susceptibility of Pea (Pisum sativum L.) and Lentil (Lens culinaris Medik.) Cultivars to Agrobacterium tumefaciens

Langitudinally sliced embryonic axes from pea and lentil mature seeds cocultivated withA. tumefaciens carrying agus reporter gene in its T-DNA provided a convenient means to evaluate the efficiency of gene transfer to tissues in different cultivars and cocultivation conditions. Use of this technique demonstrated wide variation in susceptibility toAgrobacterium among several pea and lentil commercial genotypes.

Alternative Strategies for Transitioning to Organic Production in Direct-Seeded Grain Systems in Eastern Washington I: Crop Agronomy

Organic grain production is not common to eastern Washington, but offers attractive market opportunities if systems can be developed that are compatible with regional production constraints. Erodible soils make tillage-intensive systems unsustainable, and there are limited alternative fertility options. Given these constraints, the objective of this study was to evaluate nine cropping systems during the transition to organic grain production under reduced tillage and relying on legumes as the primary source of crop nitrogen. The experimental transition systems ranged in the degree in which legume grain crops or legume green manures/forages were present in the rotation. We found that spring pea grown for grain to be a poor choice for organic production due to the susceptibility of this crop to insect pests and weeds, which generally resulted in crop failure. Likewise, bell bean was poorly suited as a legume green manure crop due to its low productivity in our region. In contrast, alfalfa and winter pea-based systems achieved the best legume biomass production, weed suppression, and crop yields in the subsequent crops. Winter wheat substantially out-yielded spring wheat, but the yields of both of these crops were well below the yield potential typically achieved under conventional production. Competition from annual weeds, such as wild oat and prickly lettuce, and the perennial weed field bindweed was difficult to manage under the reduced tillage regimes employed in this study.

A Comprehensive Survey of Soft Wheat Grain Quality in U.S. Germplasm

ABSTRACT Wheat (Triticum aestivum L.) quality is dependent upon both genetic and environmental factors, which work in concert to produce specific grain, milling, flour, and baking characteristics. This study surveyed all of the 132 soft wheat varieties (cultivars and advanced breeding lines) grown in the U.S. regional nursery system, which encompassed the three main soft wheat producing regions of the United States (eastern and southern soft red winter and western soft white). The quality parameters included test weight, kernel hardness, weight, and diameter, wheat and flour protein, polyphenol oxidase, break flour yield, flour yield, flour ash, milling score, flour swelling volume, flour SDS sedimentation volume, solvent retention capacity (SRC) for water, sodium carbonate, sucrose, and lactic acid, Rapid Visco Analyzer peak pasting viscosity, and cookie diameter. High levels of variation were observed among varieties, regions, and specific environments, with environment being in general a much greater s...