Ian C. Burke

CGA-362622 Antagonizes Annual Grass Control with Clethodim1

Abstract: Field and greenhouse experiments were conducted to evaluate clethodim, CGA-362622, mixtures thereof, and sequential treatments for control of broadleaf signalgrass, fall panicum, goosegrass, and large crabgrass. In greenhouse experiments, clethodim alone provided 93 and 100% control of three- to four-leaf goosegrass at the low (105 g ai/ha) and high (140 g/ha) rates, respectively, whereas CGA-362622 did not control grasses in greenhouse or field experiments. Control of six- to eight-leaf goosegrass in the greenhouse with clethodim was 75% for the low rate and 89% for the high rate. Control of goosegrass in greenhouse studies was reduced at least 43 percentage points with CGA-362622 and clethodim at the high rate in mixture compared with control provided by clethodim at the high rate alone. When CGA-362622 and clethodim were applied in mixture in field studies, the effectiveness of the graminicide was decreased from > 97 to < 57% control for all annual grasses. Antagonism of clethodim activity was greater than that of the tank mixture when clethodim was applied 1 d after CGA-362622 on large crabgrass, goosegrass, and fall panicum. Clethodim applied 7 d before or after CGA-362622 controlled the four grass species as well as did clethodim applied alone. When CGA-362622 was applied to goosegrass alone, fresh weight accumulation stopped for a period of 4 d compared with untreated plants. Normal growth resumed after 4 d. Nomenclature: CGA-362622, N-[(4,6-dimethoxy-2-pyrimidinyl)carbamoyl]-3-(2,2,2-trifluoroethoxy)-pyridin-2-sulfonamide sodium salt; clethodim; broadleaf signalgrass, Brachiaria platyphylla (Griseb.) Nash #3 BRAPP; fall panicum, Panicum dichotomiflorum (L.) # PANDI; goosegrass, Eleusine indica (L.) Gaertn. # ELEIN; large crabgrass, Digitaria sanguinalis (L.) Scop. # DIGSA. Additional index words: Antagonism, growth analysis, orthogonal contrasts. Abbreviations: ALS, acetolactase synthase (EC 4.1.3.18); DAT, days after treatment; POST, postemergence.

Palmer Amaranth Interference and Seed Production in Peanut

Studies were conducted to evaluate density-dependent effects of Palmer amaranth on weed and peanut growth and peanut yield. Palmer amaranth remained taller than peanut throughout the growing season and decreased peanut canopy diameter, although Palmer amaranth density did not affect peanut height. The rapid increase in Palmer amaranth height at Goldsboro correspondingly reduced the maximum peanut canopy diameter at that location, although the growth trends for peanut canopy diameter were similar for both locations. Palmer amaranth biomass was affected by weed density when grown with peanut. Peanut pod weight decreased linearly 2.89 kg/ha with each gram of increase in Palmer amaranth biomass per meter of crop row. Predicted peanut yield loss from season-long interference of one Palmer amaranth plant per meter of crop row was 28%. Palmer amaranth seed production was also described by the rectangular hyperbola model. At the highest density of 5.2 Palmer amaranth plants/m crop row, 1.2 billion Palmer amaranth seed/ha were produced. Nomenclature: Palmer amaranth, Amaranthus palmeri S. Wats. AMAPA; peanut, Arachis hypogaea L. ‘Perry’.

Annual Grass Control in Peanut (Arachis hypogaea) with Clethodim and Imazapic

Field experiments were conducted to evaluate possible interactions of clethodim with imazapic applied as mixtures or sequentially for control of broadleaf signalgrass, fall panicum, goosegrass, and large crabgrass. Imazapic at 70 g ai/ha alone controlled grass weeds inconsistently, whereas clethodim at 140 g ai/ha alone controlled the same weeds at least 99%. Imazapic did not affect broadleaf signalgrass control by clethodim. Reduced control of fall panicum, goosegrass, and large crabgrass was observed when clethodim and imazapic were applied in mixture. Antagonism of clethodim occurred when clethodim was applied 1 d before or up to 3 d after application of imazapic (fall panicum and large crabgrass). Antagonism of goosegrass control was noted when imazapic was applied 3 d before or up to 7 d after application of clethodim. In other experiments, large crabgrass and Texas panicum control by clethodim (70 and 140 g/ha) applied alone or with imazapic (70 g/ ha) or bentazon (1.1 kg ai/ha) plus 2,4-DB (0.28 kg ai/ha) either with or without ammonium sulfate (2.8 kg/ha) was evaluated. Texas panicum control by clethodim was reduced by imazapic regardless of the ammonium sulfate rate. However, large crabgrass control by imazapic was not affected in these experiments. Control of both grasses by clethodim was reduced substantially by bentazon plus 2,4-DB, although in some instances ammonium sulfate improved control when in mixture. Ammonium sulfate improved control by clethodim in some instances irrespective of the broadleaf–sedge herbicide treatments. Nomenclature: Bentazon; clethodim; 2,4-DB; imazapic; broadleaf signalgrass, Brachiaria platyphylla (Griseb) Nash #3 BRAPP; fall panicum, Panicum dichotomiflorum L. # PANDI; goosegrass, Eleusine indica L. Gaertn. # ELEIN; large crabgrass, Digitaria sanguinalis L. Scop. # DIGSA; Texas panicum, Panicum texanum Buckl. # PANTE. Additional index words: Ammonium sulfate, antagonism, herbicide compatibility, herbicide interaction, sequential application.

Weed Management in Cotton with CGA-362622, Fluometuron, and Pyrithiobac1

An experiment was conducted at five locations in North Carolina during 2000 and 2001 to evaluate weed control, crop injury, and cotton yield. Weed management systems included different combinations of pyrithiobac preemergence (PRE), fluometuron PRE, CGA-362622 postemergence (POST), pyrithiobac POST, and monosodium salt of methylarsonic acid (MSMA) plus prometryn applied late POST-directed (LAYBY). At Goldsboro in 2000, cotton was injured 74 to 78% by CGA-362622 POST when evaluated 4 to 7 d after treatment (DAT). Injury at Clayton, Goldsboro, and Lewiston in 2001 and Rocky Mount in 2000 was less than 16% 4 to 7 DAT with the same treatment and was not apparent by 62 DAT. CGA-362622 controlled common lambsquarters, common ragweed, Palmer amaranth, sicklepod, smooth pigweed, and Ipomoea species including entireleaf, ivyleaf, and pitted morningglory, and the addition of pyrithiobac to the herbicide system, either PRE or POST, increased control of Amaranthus species, jimsonweed, and prickly sida. CGA-362622 did not control jimsonweed or prickly sida. Fluometuron PRE, pyrithiobac PRE, and MSMA plus prometryn LAYBY were beneficial for increasing weed control and cotton lint yields. Prometryn plus MSMA LAYBY increased control of common ragweed, entireleaf morningglory, jimsonweed, pitted morningglory, and smooth pigweed and provided higher cotton yields than similar systems without a LAYBY. The greatest weed control and greatest cotton lint yields required complete weed management systems that included a combination of PRE, POST, and LAYBY treatments. Nomenclature: CGA-362622, N-[(4,6-dimethoxy-2-pyrimidinyl)carbamoyl]-3-(2,2,2-trifluoroethoxy)-pyridin-2-sulfonamide sodium salt; fluometuron; monosodium salt of methylarsonic acid; prometryn; pyrithiobac; common lambsquarters, Chenopodium album L. #3 CHEAL; common ragweed, Ambrosia artemisiifolia L. # AMBEL; entireleaf morningglory, Ipomoea hederacea var. integriuscula Gray # IPOHG; ivyleaf morningglory, Ipomoea hederacea (L.) Jacq. # IPOHE; jimsonweed, Datura stramonium L. # DATST; Palmer amaranth, Amaranthus palmerii L. # AMAPA; pitted morningglory, Ipomoea lacunosa L. # IPOLA; prickly sida, Sida spinosa L. # SIDSP; sicklepod, Senna obtusifolia (L.) Irwin and Barneby # CASOB; smooth pigweed, Amaranthus hybridus L. # AMACH; cotton, Gossypium hirsutum L. ‘Paymaster 1218 BG/RR’, ‘Fibermax 989’, ‘Stoneville 474’. Additional index words: Crop injury, crop yield. Abbreviations: DAT, days after treatment; EPOST, early postemergence; fb, followed by; LAYBY, late postemergence directed; MSMA, monosodium salt of methylarsonic acid; POST, postemergence; PRE, preemergence.

Absorption, translocation, and metabolism of foliar-applied CGA-362622 in purple and yellow nutsedge (Cyperus rotundus and C. esculentus)

Abstract Studies were conducted to evaluate the absorption, translocation, and metabolism of 14C–CGA-362622 when foliar-applied to purple and yellow nutsedge. Less than 53% of the herbicide was absorbed after 96 h. Both nutsedge species translocated appreciable amounts of herbicide (30%) out of treated leaves. Translocation was both acropetal and basipetal, with at least 25% transported basipetally. Neither nutsedge species translocated more than 4% of applied radioactivity to the tubers and roots. Most of the metabolites formed by the nutsedge species were more polar than 14C–CGA-362622 and averaged 69 and 61% of the radioactivity in purple and yellow nutsedge, respectively. The half-life of CGA-362622 was estimated at 4 h in both purple and yellow nutsedge. Nomenclature: CGA-362622, N-([4,6-dimethoxy-2-pyrimidinyl]carbamoyl)-3-(2,2,2,-trifluoroethoxy)-pyridin-2-sulfonamide sodium salt; purple nutsedge, Cyperus rotundus L. CYPRO; yellow nutsedge, Cyperus esculentus L. CYPES.

Single nucleotide mutation in the barley acetohydroxy acid synthase (AHAS) gene confers resistance to imidazolinone herbicides

Induced mutagenesis can be an effective way to increase variability in self-pollinated crops for a wide variety of agronomically important traits. Crop resistance to a given herbicide can be of practical value to control weeds with efficient chemical use. In some crops (for example, wheat, maize, and canola), resistance to imidazolinone herbicides (IMIs) has been introduced through mutation breeding and is extensively used commercially. However, this production system imposes plant-back restrictions on rotational crops because of herbicide residuals in the soil. In the case of barley, a preferred rotational crop after wheat, a period of 9–18 mo is required. Thus, introduction of barley varieties showing resistance to IMIs will provide greater flexibility as a rotational crop. The objective of the research reported was to identify resistance in barley for IMIs through induced mutagenesis. To achieve this objective, a sodium azide-treated M2/M3 population of barley cultivar Bob was screened for resistance against acetohydroxy acid synthase (AHAS)-inhibiting herbicides. The phenotypic screening allowed identification of a mutant line showing resistance against IMIs. Molecular analysis identified a single-point mutation leading to a serine 653 to asparagine amino acid substitution in the herbicide-binding site of the barley AHAS gene. The transcription pattern of the AHAS gene in the mutant (Ser653Asn) and WT has been analyzed, and greater than fourfold difference in transcript abundance was observed. Phenotypic characteristics of the mutant line are promising and provide the base for the release of IMI-resistant barley cultivar(s).

Weed Management in Glyphosate-Resistant Corn with Glyphosate, Halosulfuron, and Mesotrione1

Four field studies were conducted at the Peanut Belt Research Station near Lewiston Woodville, NC, in 2000, 2001, and 2002 to evaluate crop tolerance, weed control, grain yield, and net returns in glyphosate-resistant corn with various herbicide systems. Preemergence (PRE) treatment options included no herbicide, atrazine at 1.12 kg ai/ha, or atrazine plus metolachlor at 1.68 kg ai/ha. Postemergence (POST) treatment options included glyphosate at 1.12 kg ai/ha as either the isopropylamine salt or the diammonium salt, either alone or in mixtures with mesotrione at 105 g ai/ha plus crop oil concentrate at 1% (v/v) or halosulfuron at 53 g ai/ha plus 0.25% (v/v) nonionic surfactant. All response variables were independent of glyphosate formulation. Addition of metolachlor to atrazine PRE improved large crabgrass and goosegrass control but did not always improve Texas panicum control. POST control of these annual grasses was similar with glyphosate alone or in mixture with halosulfuron or mesotrione. Glyphosate POST controlled common lambsquarters and common ragweed 89 and 93%, respectively. Glyphosate plus halosulfuron POST provided more effective yellow nutsedge control than glyphosate POST. Atrazine PRE or atrazine plus metolachlor PRE followed by any glyphosate POST treatment controlled Ipomoea spp. at least 93%. Glyphosate plus mesotrione in total POST systems always provided greater control of Ipomoea spp. than glyphosate alone. The highest yielding treatments always included glyphosate POST, either with or without a PRE herbicide treatment. Similarly, systems that included any glyphosate POST treatment had the highest net returns. Nomenclature: Atrazine; glyphosate; halosulfuron; mesotrione; metolachlor; common lambsquarters, Chenopodium album L. #3 CHEAL; common ragweed, Ambrosia artemisiifolia L. # AMBEL; goosegrass, Eleusine indica (L.) Gaertn. # ELEIN; large crabgrass, Digitaria sanguinalis (L.) Scop. # DIGSA; Texas panicum, Panicum texanum Buckl. # PANTE; yellow nutsedge, Cyperus esculentus L.; corn, Zea mays L. # ZEAMX. Additional index words: Diammonium salt, isopropylamine salt, net returns. Abbreviations: ALS, acetolactate synthase; DAT, days after early postemergence treatment; fb, followed by; GR, glyphosate-resistant; POST, postemergence; PRE, preemergence.

Influence of environmental factors on slender amaranth (Amaranthus viridis) germination

Abstract Germination response of slender amaranth to temperature, solution pH, moisture stress, and depth of emergence was evaluated under controlled environmental conditions. Results indicated that 30 C was the optimum constant temperature for germination. Germination of slender amaranth seed at 21 d was similar, with 35/25, 35/20, 30/25, and 30/20 alternating temperature regimes. As temperatures in alternating regimes increased, time to onset of germination decreased and rate of germination increased. Slender amaranth germination was greater with acidic than with basic pH conditions. Germination declined with increasing water stress and was completely inhibited at water potentials below −0.6 MPa. Slender amaranth emergence was greatest at depths of 0.5 to 2 cm, but some seeds emerged from as deep as 6 cm. Information gained in this study will contribute to an integrated control program for slender amaranth. Nomenclature: Slender amaranth, Amaranthus viridis L. AMAVI.

Enhanced Atrazine Degradation: Evidence for Reduced Residual Weed Control and a Method for Identifying Adapted Soils and Predicting Herbicide Persistence

Abstract Soilborne bacteria with novel metabolic abilities have been linked with enhanced atrazine degradation and complaints of reduced residual weed control in soils with an s-triazine use history. However, no field study has verified that enhanced degradation reduces atrazines residual weed control. The objectives of this study were to (1) compare atrazine persistence and prickly sida density in s-triazine-adapted and nonadapted field sites at two planting dates; (2) utilize original and published data to construct a diagnostic test for identifying s-triazine-adapted soils; and (3) develop and validate an s-triazine persistence model based on data generated from the diagnostic test, i.e., mineralization of ring-labeled 14C-s-triazine. Atrazine half-life values in s-triazine-adapted soil were at least 1.4-fold lower than nonadapted soil and 5-fold lower than historic estimates (60 d). At both planting dates atrazine reduced prickly sida density in the nonadapted soils (P ≤ 0.0091). Conversely, in the s-triazine-adapted soil, prickly sida density was not different between no atrazine PRE and atrazine PRE at the March 15 planting date (P  =  0.1397). A lack of significance in this contrast signifies that enhanced degradation can reduce atrazines residual control of sensitive weed species. Analyses of published data indicate that cumulative mineralization in excess of 50% of C0 after 30 d of incubation is diagnostic for enhanced s-triazine degradation. An s-triazine persistence model was developed and validated; model predictions for atrazine persistence under field conditions were within the 95% confidence intervals of observed values. Results indicate that enhanced atrazine degradation can decrease the herbicides persistence and residual activity; however, coupling the diagnostic test with the persistence model could enable weed scientists to identify s-triazine-adapted soils, predict herbicide persistence under field conditions, and implement alternative weed control strategies in affected areas if warranted. Nomenclature: Atrazine; prickly sida, Sida spinosa L.

Influence of environmental factors on broadleaf signalgrass (Brachiaria platyphylla) germination

Abstract Laboratory and greenhouse studies were conducted to determine the effect of temperature, solution pH, water stress, and planting depth on broadleaf signalgrass germination. Broadleaf signalgrass seed required removal of the husk for germination. When treated with constant temperature, broadleaf signalgrass germinated over a range of 20 to 35 C, with optimum germination occurring at 30 and 35 C. Onset, rate, and total germination (87%) was greatest in an alternating 20/30 C temperature regime. Germination decreased as solution pH increased, with greatest germination occurring at pH values of 4 and 5. Germination decreased with increasing water potential, and no germination occurred below − 0.8 mPa. Emergence was above 42% when seed were placed on the soil surface or buried 0.5 cm deep. Germination decreased with burial depth, but 10% of broadleaf signalgrass seed emerged from 6.0-cm depth. No seed emerged from 10-cm depth. These data suggest that broadleaf signalgrass may emerge later in the season, after rains, and could germinate rapidly and in high numbers. These attributes could contribute to poor control later in the season by soil-applied herbicides or allow broadleaf signalgrass to emerge after final postemergence treatments were made. Nomenclature: Broadleaf signalgrass, Brachiaria platyphylla (Griseb.) Nash BRAPP.