Eko Suhartono

Advance Oxidation Protein Products (AOPPs) of Newborn at Risk of Sepsis as Novel Parameter for Early-Onset Neonatal Sepsis

years , and this study proposed another parameter to detect early-onset neonatal sepsis with the use of advance oxidation protein products (AOPPs). This study was conducted in April - June 2012, saliva and serum specimens were taken from 81 newborns, in which 39 infants were at risk of sepsis and 42 infants were healthy and served as a control group. Data was analyzed by using Mann-Whitney test and Spearman correlation test. This study suggested that there is significant difference between AOPPs level on the case group compare to the control group. This study also found that there is significant difference between salivary AOPPs level compare to serum AOPPs level. As the conclusion, this study proposed that the AOPPs parameter may be used as another marker to detect early-onset neonatal sepsis.

Toll-like Receptor 2 , Toll-like Receptor 4 Expression and Phagocytic Activity of Neutrophil in Saliva of New Born Babies With Sepsis Risk, An Early Detection Of Neonatal Sepsis

Neonatal sepsis remains a leading cause of neonatal morbidity and mortality in infant, especially preterm and low birth weight baby. Sepsis is a clinical manifestation of a dysregulated immune response to invasive pathogen.There is increasing evidence that Toll-like receptors (TLRs) play a key role in the mediation of systemic responses to invading pathogens during sepsis. TLRs are essential for triggering the hosts immune response, acting as a sensor against invading pathogens. TLR2 was identified as a receptor for gram-positive peptidoglycan and bacterial lipopeptides, whereas TLR4 is part of a receptor complex recognizing gram- negative bacterial. Saliva is an important body fluid for detecting the physiological and pathological situations of the human body. Neutrophils are considered to participate in the acute response against pathogens in many tissues, and influx into the oral cavity occurs at any time. The expression and function of TLRs greatly influence the quality and control of innate immune response in patients with infectious disease. The high expression of TLRs and phagocytic activity of neutrophil saliva upon presentation of early onset neonatal sepsis patients, suggests

PENGARUH LANSOPRAZOL DAN OMEPRAZOL TERHADAP AKTIVITAS ENZIM KATALASE HEPAR TIKUS

Abstract: Lansoprazole and omeprazole is a Proton Pump Inhibitor class of drugs that are often used for the treatment of peptic ulcers. Lansoprazole and omeprazole have an influence on the various target organs exposed. Mechanism of lansoprazole and omeprazole in influencing the activity of the enzyme catalase is competing with absolute catalase enzyme substrate (H 2 O 2 ) in the binding of the enzyme active site. This study aims to determine the effect of lansoprazole and omeprazole against liver catalase enzyme activity. This study was a laboratory experimental study conducted in three groups, namely the control group (P0), the treatment group (P1) is given lansoprazole 30 mg and a treatment group (P2) given omeprazole 20 mg. The result showed the value of Km of the control group (P0) of 13.482 mmol/dm 3 , the treatment group (P1) of 11,227 mmol/dm 3 and the treatment group (P2) of 6,327 mmol/dm 3 . Analysis of statistical data shows the regression correlation value of p for P1 was 0,01 adn for P2 was 0,02 (p <0.05) and the R value approaching 1 with a linear graph Lineweaver Burk meaningful. Concluded that lansoprazole and omeprazole may affect the activity of the liver enzyme catalase. Keywords: lansoprazole, omeprazole, enzyme catalase, rat liver Abstrak: Lansoprazol dan omeprazol merupakan obat golongan proton pump inhibitor yang sering digunakan untuk pengobatan tukak lambung. Lansoprazol dan omeprazol memiliki berbagai pengaruh terhadap organ target yang terpajan. Mekanisme lansoprazol dan omeprazol dalam mempengaruhi aktivitas enzim katalase adalah berkompetisi dengan substrat absolut enzim katalase (H 2 O 2 ) dalam mengikat sisi aktif enzim. Penelitian ini bertujuan untuk mengetahui pengaruh lansoprazol dan omeprazol terhadap aktivitas enzim katalase hepar. Penelitian ini merupakan penelitian quasi eksperimental yang dilakukan pada 3 kelompok, yakni kelompok kontrol (P0), kelompok perlakuan (P1) diberikan lansoprazol 30 mg dan kelompok perlakuan (P2) diberikan omeprazol 20 mg. Hasil penelitian didapatkan nilai Km pada kelompok kontrol (P0) sebesar 13,482 mmol/dm 3 , pada kelompok perlakuan (P1) sebesar 11,227mmol/dm 3 dan pada kelompok perlakuan (P2) sebesar 6,327mmol/dm 3 . Analisis data statistik korelasi regresi menunjukkan nilai p pada P1 0,01 dan pada P2 0,02 (p<0,05) serta nilai R mendekati 1 dengan grafik linear Lineweaver Burk yang menanjak. Disimpulkan bahwa lansoprazol dan omeprazol dapat mempengaruhi aktivitas enzim katalase hepar mencit. Kata-kata kunci : lansoprazol, omeprazol, enzim katalase, hepar mencit