Eleazar M. Escamilla-Silva

Selective production of bikaverin in a fluidized bioreactor with immobilized Gibberella fujikuroi

The best culture medium composition for the production of bikaverin by Gibberella fujikuroi in shake-flasks, i.e. 100 g glucose l−1; 1 g NH4Cl l−1; 2 g rice flour l−1; 5 g KH2PO4 l−1 and 2.5 g MgSO4 l−1, was obtained through a fractional factorial design and then scaled-up to a fluidized bioreactor. The effects of carbon and nitrogen concentrations, inoculum size, aeration, flow rate and bead sizes on batch bikaverin production using immobilized G. fujikuroi in a fluidized bioreactor were determined by an orthogonal experimental design. Concentrations of up to 6.83 g bikaverin l−1 were obtained when the medium contained 100 g glucose l−1 and 1 g NH4Cl l−1 with an inoculum ratio of 10% v/v, an aeration rate of 3 volumes of air per volume of medium min−1, and a bead size of 3 mm. Based on dry weight, the bikaverin production was 30–100 times larger than found in submerged culture and approximately three times larger than reported for solid substrate fermentation.

A possible mechanism of metabolic regulation in Gibberella fujikuroi using a mixed carbon source of glucose and corn oil inferred from analysis of the kinetics data obtained in a stirrer tank bioreactor

A nonstructured model was used to study the dynamics of gibberellic acid production in a stirred tank bioreactor. Experimental data were obtained from submerged batch cultures of Gibberella fujikuroi (CDBB H‐984) grown in varying ratios of glucose‐corn oil as the carbon source. The nitrogen depletion effect was included in mathematical model by considering the specific kinetic constants as a linear function of the normalized nitrogen consumption rate. The kinetics of biomass growth and consumption of phosphate and nitrogen were based on the logistic model. The traditional first‐order kinetic model was used to describe the specific consumption of glucose and corn oil. The nitrogen effect was solely included in the phosphate and corn oil consumption and biomass growth. The model fit was satisfactory, revealing the dependence of the kinetics with respect to the nitrogen assimilation rate. Through simulations, it was possible to make diagrams of specific growth rate and specific rate of substrate consumptions, which was a powerful tool for understanding the metabolic interactions that occurred during the various stages of fermentation process. This kinetic analysis provided the proposal of a possible mechanism of regulation on growth, substrate consumptions, and production of gibberellic acid (GA3) in G. fujikuroi.

Hydrodynamics and Mass Transfer Simulation in Airlift Bioreactor with Settler using Computational Fluid Dynamics

Abstract In this work, the effect of inlet-gas superficial velocity over the circulation liquid velocity, gas holdup and mass transfer, from an airlift bioreactor with settler were studied by Computational Fluid Dynamics (CFD) modeling and contrasted with experimental results. Multiphase mixture model and κ-ε turbulence model were used to describe the two phases gas-liquid flow pattern in airlift bioreactor. The hydrodynamic parameters such as liquid circulation velocity and gas holdup were computed by solving the governing equations of continuity, moment and turbulence transport using the finite volume method. Global mass transfer coefficient was evaluated through the Higbie’s penetration theory and the two-phase fluid dynamic theory. Comparison between our numerical data and experimental data previously reported in the literature was done. Numerical and experimental data were very close, and the differences found were discussed in terms of the limitations of this study.