Elena A. Ignatova

Support from DNA data for a narrow species concept in Schistidium (Grimmiaceae, Musci)

Abstract A study of ITS1 of 28 specimens of eight species of Schistidium from well-separated populations in Russia and northwest Europe revealed that there are very big differences between species (up to 16 substitutions and 256 indels), whereas within the species differences in DNA there are very few (none to four substitutions and none to six indels). These results strongly support the narrow species concept in Schistidium. Schistidium papillosum is represented by two quite distinct genotypes and probably needs further splitting.

New national and regional bryophyte records, 46

L. T. Ellis1, A. K. Asthana2, P. Srivastava2, I. Omar2, K. K. Rawat2, V. Sahu2, M. J. Cano3, D. P. Costa4, E. M. Dias5,8, N. Dias dos Santos5,6, J. B. Silva5, V. E. Fedosov7, M. N. Kozhin7, E. A. Ignatova7, S. R. Germano8, E. O. Golovina9, N. J. M. Gremmen10, R. Ion11, S. Ştefǎnuţ11, M. von Konrat12, M. S. Jimenez13,14, G. M. Suárez13,15, T. Kiebacher16, M. Lebouvier17, D. G. Long18, D. Maity19, R. Ochyra20, I. Parnikoza21, V. Plášek22, L. Fialová22, Z. Skoupá22, S. Poponessi23, M. Aleffi23, M. S. Sabovljević24, A. D. Sabovljević24, P. Saha25, M. N. Aziz25, J. Sawicki26,22, M. Suleiman27, B.-Y. Sun28, J. Váňa29, T. Wójcik30, Y.-J. Yoon31, J. Żarnowiec32, J. Larraín33

The phylogeny of Schistidium (Bryophyta, Grimmiaceae) based on the primary and secondary structure of nuclear rDNA internal transcribed spacers

Phylogeny of Schistidium (Bryophyta, Grimmiaceae) was studied by comparing the nucleotide sequences of internal transcribed spacers ITS1-2 of nuclear rDNA and the trnT-trnD region of chloroplast DNA. Phylogenetic trees constructed based on nuclear and chloroplast sequences were consistent, comprising a basal grade and two large clades. Morphological characteristics specific for these clades were described. Secondary structures of ITS1 and ITS2 Schistidium species were modeled using thermodynamic criteria. Four different structures of the longest ITS1 hairpin were identified. These results were used to analyze possible paths of Schistidium evolution. Characteristics of the ITS2 secondary structure support the two major clades recognized in the phylogenetic trees.

Bryophyte flora of the Volzhsko-Kamskiy Nature Reserve (Tatarstan, European Russia)

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New national and regional bryophyte records, 51

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RPS4 and NAD5 sequences evidenced of polyphyly of ditrichaceae and parallelisms in the evolution of haplolepidous mosses

L. T. Ellis1, M. Aleffi2, H. Bednarek-Ochyra3, V. A. Bakalin4, M. Boiko5, J. A. Calleja6,7, V. E. Fedosov8, M. S. Ignatov8, E. A. Ignatova8, R. Garilleti9, T. Hallingbäck10, N. Lönnell10, N. Hodgetts11, T. Kiebacher12, J. Larraín13, M. Lebouvier14, M. Lüth15, V. Mazimpaka16, B. Vigalondo16, F. Lara16, R. Natcheva17, M. Nobis18, A. Nowak19, J. D. Orgaz20, J. Guerra20, J. Pantović21, N. Nikolić21, M. S. Sabovljević21, A. D. Sabovljević21, O. Yu. Pisarenko22, V. Plášek23, Z. Skoupá23, S. Poponessi24, M. Privitera25, M. Puglisi25, M. Skudnik26, Q. H. Wang27

Structure of Intergenic Spacer IGS1 of Ribosomal Operon from Schistidium Mosses

Molecular phylogenetic analysis of 66 representatives of haplolepidous mosses showed polyphylia of Ditrichaceae. According to the data obtained, the structure of the peristome, as well as features of the gametophyte on which a family traditionally allocated, arose independently in different groups of haplolepideous mosses. At least six genera (Distichium, Saelania, Eccremidium, Garckea, Rhamphidium, and Wilsoniella) should be excluded from the Ditrichaceae family, while Saelania and Distichium should be assigned even to another order. The loss of the peristome and forming of cleistocarpous capsules also occurs independently in at least two lineages of Ditrichaceae s. str., and in representatives of several lineages of Pottiaceae, a family derived from this group. Ditrichum, the type genus of Ditrichaceae, is also polyphyletic, species of this genus belong to two clades. It was concluded that parallel lines of the morphological variability in this group of mosses occur and its phylogeny need to be resolved based on molecular data.

New national and regional bryophyte records, 55

The structure of the intergenic spacer 1 (IGS1) of the ribosomal operon from 12 species of Schistidium mosses was studied. In the IGS1 sequences of these species, three conserved regions and two areas of GCand A-enriched repeats were identified. All of the studied mosses have a conserved pyrimidine-enriched motif at the 5′-end of IGS1. Species-specific nucleotide substitutions and insertions were found in the conserved areas. The repeated units contain single nucleotide substitutions that make unique the majority of repeated units. The positions of such repeats in IGS1 are species-specific, but their number can vary within the species and among operons of the same specimen. The comparison of IGS1 sequences from the Schistidium species and from representatives of ten other moss genera revealed the presence of common conserved motifs with similar localization. Presumably, these motifs are elements of termination of the pre-rRNA transcription and processing of rRNA.

New national and regional bryophyte records, 56

L. T. Ellis, O. M. Afonina, M. Aleffi, R. L. Andriamiarisoa, M. Bačkor, M. Goga, H. Bednarek-Ochyra, D. A. Callaghan, P. Campisi, M. G. Dia, M. L. Marino, J. Enroth, P. Erzberger, V. Hugonnot, E. A. Ignatova, T. Kiebacher, J. Kučera, M. Lebouvier, G. M. Maria, S. Ştefănuţ, J. Nagy, T. Pócs, S. Poponessi, R. Venanzoni, D. Gigante, F. Prosser, C. Reeb, M. S. Sabovljević, J. R. Shevock, S. Shirzadian, S. Akhoondi Darzikolaei, E. R. F. Souza, A. Silva Pinto, J. B. Silva, S. F. Lopes, K. Torzewski, A. Kazienko

Coscinodon monchiquensis R.D.Porley, Ochyra & Ignatova (Grimmiaceae), a new species from the Algarve, southern Portugal

Systematische und Evolutionäre Botanik, Universität Zürich, Zürich, Switzerland, California Academy of Sciences, San Francisco, CA, USA, Institute of Biology Bucharest of Romanian Academy, Bucharest, Romania, Xinjiang University, Urumqi, People’s Republic of China, Chonbuk National University, Jeonju, Korea, Institute of General and Experimental Biology SD RAS, Ulan-Ude, Russia, Department of Botany, Charles University, Praha, Czech Republic, Department of Geobotany and Plant Ecology, University of Łódź, Łódź, Poland, Endemic Species Research Institute, Nantou, Taiwan, Korea Polar Research Institute, Incheon,