Elena B. Kiseleva
Nizhny Novgorod State Medical Academy
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Publication
Featured researches published by Elena B. Kiseleva.
Journal of Biophotonics | 2011
Natalia D. Gladkova; Olga Streltsova; Elena V. Zagaynova; Elena B. Kiseleva; Valentin M. Gelikonov; Grigory V. Gelikonov; Maria Karabut; Katerina Yunusova; Olga Evdokimova
The capabilities of cross-polarization optical coherence tomography (CP OCT) for early bladder-cancer detection are assessed in statistical study and compared with the traditional OCT. Unlike the traditional OCT that demonstrates images only in copolarization, CP OCT acquires images in cross-polarization and copolarization simultaneously. 116 patients with localized flat suspicious lesions in the bladder were enrolled, 360 CP OCT images were obtained and analyzed. CP OCT demonstrated sensitivity 93.7% (vs. 81.2%, <0.0001), specificity 84% (vs. 70.0%, <0.001) and accuracy 85.3% (vs. 71.5%, <0.001) in detecting flat malignant bladder lesions, which is significantly better than with the traditional OCT. Higher diagnostic efficacy of CP OCT in detecting early bladder cancer is associated with the ability to detect changes in epithelium and connective tissues.
Biomedical Optics Express | 2015
Elena B. Kiseleva; Mikhail Yu. Kirillin; Felix I. Feldchtein; Alex Vitkin; Ekaterina A. Sergeeva; Elena V. Zagaynova; Olga Streltzova; Boris E. Shakhov; Ekaterina V. Gubarkova; Natalia D. Gladkova
Quantitative image analysis and parameter extraction using a specific implementation of polarization-sensitive optical coherence tomography (OCT) provides differential diagnosis of mucosal pathologies in in-vivo human bladders. We introduce a cross-polarization (CP) OCT image metric called Integral Depolarization Factor (IDF) to enable automatic diagnosis of bladder conditions (assessment the functional state of collagen fibers). IDF-based diagnostic accuracy of identification of the severe fibrosis of normal bladder mucosa is 79%; recurrence of carcinoma on the post-operative scar is 97%; and differentiation between neoplasia and acute inflammation is 75%. The promising potential of CP OCT combined with image analysis in human urology is thus demonstrated in vivo.
Journal of Biophotonics | 2013
Natalia D. Gladkova; Elena B. Kiseleva; Natalia Robakidze; Irina V. Balalaeva; Maria Karabut; Ekaterina V. Gubarkova; Felix I. Feldchtein
The goal of the research was analysis of the effect of collagen condition in formation of cross-polarized CP OCT images. We used of the CP OCT technique for studying collagen condition on an example of oral mucosa. Special histologic picrosirius red (PSR) staining of cheek mucosa specimens was used with subsequent assessing of the result of collagen staining in polarized light. High correlation (r = 0.692, p = 0.0001) between OCT signal standard deviation (SD) in cross-polarized images and brightness of PSR stained collagen fibers in cheek mucosa specimens was demonstrated in patients with inflammatory intestine and oral mucosa diseases. We have found that the OCT signal SD in cross-polarized images reflects two boundary conditions of collagen disorganization, namely, loss of fiber properties at active inflammation which attenuates the signal and fibrosis that occurs due to synthesis of a new remodeled collagen which amplifies the OCT signal.
Journal of Biophotonics | 2013
Natalia D. Gladkova; Elena B. Kiseleva; Olga Streltsova; Natalia Prodanets; Ludmila B. Snopova; Maria Karabut; Ekaterina V. Gubarkova; Elena V. Zagaynova
The combined use of fluorescence cystoscopy and cross-polarization optical coherence tomography (CP OCT) with quantitative estimation of the OCT signal was assessed in 92 bladder zones. It demonstrated the diagnostic accuracy in detecting superficial bladder cancer of 93.6%, sensitivity 96.4%, specificity 92.1%, positive predictive value 87% and negative predictive value 97.9%. Quantitative estimation of OCT signal standard deviation in cross-polarization (CP OCT SD index) makes the visual criteria of CP OCT image assessment more objective. The level of CP OCT SD index for diagnosing superficial bladder cancer, including cancer in situ, was 4.32 dB and lower. When tumor is located on a postoperative scar, CP OCT SD index may be higher than the threshold level of 4.32 dB due to strong scattering and depolarization in scar fibrous tissue. A high inverse correlation was found between CP OCT SD index and the level expressed by p63, Ki-67, p53, CD44v6 markers.
Journal of Biophotonics | 2016
Ekaterina V. Gubarkova; Varvara V. Dudenkova; Felix I. Feldchtein; Lidia B. Timofeeva; Elena B. Kiseleva; Sergei S. Kuznetsov; Boris E. Shakhov; Alexander A. Moiseev; Valentin M. Gelikonov; Gregory V. Gelikonov; Alex Vitkin; Natalia D. Gladkova
We combined cross-polarization optical coherence tomography (CP OCT) and non-linear microscopy based on second harmonic generation (SHG) and two-photon-excited fluorescence (2PEF) to assess collagen and elastin fibers and other vascular structures in the development of atherosclerosis, including identification of vulnerable plaques, which remains an important clinical problem and imaging application. CP OCTs ability to visualize tissue birefringence and cross-scattering adds new information about the microstructure and composition of the plaque. However its interpretation can be ambiguous, because backscattering contrast may have a similar appearance to the birefringence related fringes. Our results represent a step towards minimally invasive characterization and monitoring of different stages of atherosclerosis, including vulnerable plaques. CP OCT image of intimal thickening in the human coronary artery. The dark stripe in the cross-polarization channel (arrow) is a polarization fringe related to the phase retardation between two eigen polarization states. It is histologically located in the area of the lipid pool, however this stripe is a polarization artifact, rather than direct visualization of the lipid pool.
International Journal of Radiation Biology | 2015
Anna Maslennikova; M. Kochueva; Natalia Yu. Ignatieva; Alex Vitkin; O. L. Zakharkina; Vladislav A. Kamensky; Ekaterina A. Sergeeva; Elena B. Kiseleva; V. N. Bagratashvili
Abstract Purpose: To evaluate the dose-time dependences of structural changes occurring in collagen within 24 hours to three months after gamma-irradiation at doses from 2–40 Gy in vivo. Materials and methods: Rats tail tendon was chosen as in vivo model, with its highly ordered collagen structure allowing the changes to be interpreted unambiguously. Macromolecular level (I) was investigated by differential scanning calorimetry (DSC); fibers and bundles level (II) by laser scanning microscopy (LSM), and bulk tissue microstructural level (III) by cross-polarization optical coherence tomography (CP-OCT). Results: For (I), the formation of molecular cross-links and breaks appeared to be a principal mechanism of collagen remodeling, with the cross-links number dependent on radiation dose. Changes on level (II) involved primary, secondary and tertiary bundles splitting in a day and a week after irradiation. Bulk collagen microstructure (III) demonstrated early widening of the interference fringes on CP-OCT images observed to occur in the tendon as result of this splitting. At all three levels, the observed collagen changes demonstrated complete remodeling within ∼ a month following irradiation. Conclusion: The time course and dose dependencies of the observed collagen changes at different levels of its hierarchy further contribute to elucidating the role of connective tissue in the radiotherapy process.
Scientific Reports | 2017
Marina A. Sirotkina; L. A. Matveev; Marina V. Shirmanova; Vladimir Yu. Zaitsev; N. L. Buyanova; V. V. Elagin; Grigory V. Gelikonov; S. S. Kuznetsov; Elena B. Kiseleva; Alexander A. Moiseev; S. V. Gamayunov; Elena V. Zagaynova; Felix I. Feldchtein; Alex Vitkin; Natalia D. Gladkova
Photodynamic therapy (PDT) is a promising modern approach for cancer therapy with low normal tissue toxicity. This study was focused on a vascular-targeting Chlorine E6 mediated PDT. A new angiographic imaging approach known as M-mode-like optical coherence angiography (MML-OCA) was able to sensitively detect PDT-induced microvascular alterations in the mouse ear tumour model CT26. Histological analysis showed that the main mechanisms of vascular PDT was thrombosis of blood vessels and hemorrhage, which agrees with angiographic imaging by MML-OCA. Relationship between MML-OCA-detected early microvascular damage post PDT (within 24 hours) and tumour regression/regrowth was confirmed by histology. The advantages of MML-OCA such as direct image acquisition, fast processing, robust and affordable system opto-electronics, and label-free high contrast 3D visualization of the microvasculature suggest attractive possibilities of this method in practical clinical monitoring of cancer therapies with microvascular involvement.
Journal of Biomedical Optics | 2016
Ekaterina V. Gubarkova; Mikhail Kirillin; Varvara V. Dudenkova; P. S. Timashev; Svetlana L. Kotova; Elena B. Kiseleva; Lidia B. Timofeeva; Galina V. Belkova; Anna B. Solovieva; Alexander A. Moiseev; Gregory V. Gelikonov; Ilya I. Fiks; Felix I. Feldchtein; Natalia D. Gladkova
Abstract. A combination of approaches to the image analysis in cross-polarization optical coherence tomography (CP OCT) and high-resolution imaging by nonlinear microscopy and atomic force microscopy (AFM) at the different stages of atherosclerotic plaque development is studied. This combination allowed us to qualitatively and quantitatively assess the disorganization of collagen in the atherosclerotic arterial tissue (reduction and increase of CP backscatter), at the fiber (change of the geometric distribution of fibers in the second-harmonic generation microscopy images) and fibrillar (violation of packing and different nature of a basket-weave network of fibrils in the AFM images) organization levels. The calculated CP channel-related parameters are shown to have a statistically significant difference between stable and unstable (also called vulnerable) plaques, and hence, CP OCT could be a potentially powerful, minimally invasive method for vulnerable plaques detection.
Scientific Reports | 2017
Anna Maslennikova; Marina A. Sirotkina; Alexander A. Moiseev; E. S. Finagina; Sergey Yu. Ksenofontov; Grigory V. Gelikonov; L. A. Matveev; Elena B. Kiseleva; Vladimir Yu. Zaitsev; Elena V. Zagaynova; Felix I. Feldchtein; Natalia D. Gladkova; Alex Vitkin
Mucositis is the limiting toxicity of radio(chemo)therapy of head and neck cancer. Diagnostics, prophylaxis and correction of this condition demand new accurate and objective approaches. Here we report on an in vivo longitudinal monitoring of the oral mucosa dynamics in 25 patients during the course of radiotherapy of oropharyngeal and nasopharyngeal cancer using multifunctional optical coherence tomography (OCT). A spectral domain OCT system with a specially-designed oral imaging probe was used. Microvasculature visualization was based on temporal speckle variations of the full complex signal evaluated by high-pass filtering of 3D data along the slow scan axis. Angiographic image quantification demonstrated an increase of the vascular density and total length of capillary-like-vessels before visual signs or clinical symptoms of mucositis occur. Especially significant microvascular changes compared to their initial levels occurred when grade two and three mucositis developed. Further, microvascular reaction was seen to be dose-level dependent. OCT monitoring in radiotherapy offers a non-invasive, convenient, label-free quantifiable structural and functional volumetric imaging method suitable for longitudinal human patient studies, furnishing fundamental radiobiological insights and potentially providing useful feedback data to enable adaptive radiotherapy (ART).
Journal of Biophotonics | 2018
Alexander A. Moiseev; Sergey Yu. Ksenofontov; Marina A. Sirotkina; Elena B. Kiseleva; Maria Gorozhantseva; Natalia M. Shakhova; L. A. Matveev; Vladimir Yu. Zaitsev; Alexander L. Matveyev; Elena V. Zagaynova; Valentin M. Gelikonov; Natalia D. Gladkova; Alex Vitkin; Grigory V. Gelikonov
This work is dedicated to the development of the OCT system with angiography for everyday clinical use. Two major problems were solved during the development: compensation of specific natural tissue displacements, induced by contact scanning mode and physiological motion of patients (eg, respiratory and cardiac motions) and online visualization of vessel cross-sections to provide feedback for the system operator.