Elisabetta Campeol

In vitro antimicrobial activity of extracts and isolated constituents of Geum rivale.

The antimicrobial activity of extracts of Geum rivale (Rosaceae) and that of some isolated constituents, on bacteria and fungi, was evaluated. The activity was concentrated in the triterpenes fraction and, for gram+ and gram− bacteria, also in the flavonoids fraction. Copyright

A New HPLC‐ELSD Method To Quantify Indican in Polygonum tinctorium L. and To Evaluate β‐Glucosidase Hydrolysis of Indican for Indigo Production

A method to quantify the indigo precursor indican (indoxyl‐β‐d‐glucoside) in Polygonum tinctoriumL. has been developed. Plant material was extracted in deionized water, and indican was identified and quantified using high performance liquid chromatography (HPLC) coupled to an evaporative light scattering detector (ELSD). Results confirmed that with this method it is possible to measure indican content in a short time, obtaining reliable and reproducible data. Using this method, leaf indican content was quantified every 15 days during the growing season (from May to October) in P. tinctorium crops grown in a field experiment in Central Italy. Results showed that indican increased along the growing season until flowering and was positively affected by photosynthetic active radiation (PAR). Indican is naturally hydrolyzed by native β‐glucosidase to indoxyl and glucose, the indoxyl yielding indigo. The activity of two enzymes, sweet almond β‐glucosidase and Novarom G preparation, were compared with P. tinctorium native β‐glucosidase to evaluate indigo production. Results showed that the ability to promote indigo formation increased as follows: almond β‐glucosidase ≤ Novarom G.

Flavonoids analysis of four Vicia species of Narbonensis complex in two different vegetative phases

Abstract A qualitative analysis of flavonoids has been carried out for Vicia eristalioides Maxt., V. kalakhensis Khatt., Maxt. and Bisby, V. narbonensis L. and V. galilaea Plitm. et Zoh in two different vegetative phases. Free aglycones were consistently absent and there was no evidence of flavone glycosides, whereas kaempferol and quercetin glycosides were present in different amounts, according to the species. The flavonoid patterns of the four species were compared and differences were present among them and also within the same species during the two vegetative phases. The results indicate that flavonoid data may be used in the study of the organisation and evolution in Narbonensis complex.

Flavonoids analysis of Vicia species of Narbonensis complex: V. kalakhensis Khatt., Maxt. & Bisby and V. eristalioides Maxt

Abstract A qualitative and quantitative analysis of flavonoids has been carried out for first time in Vicia eristalioides Maxt. and in Vicia kalakhensis Khatt., Maxt. Bisby. Free aglycones were consistently absent from both species while kaempferol derivatives were pre dominant in V. kalakhensis; a more complex mixture of flavonoid glycosides,(kaempferoland quercetin glycosides) was present in V. eristalioides. Therer was no evidence of flavones glycosides.The flavonoid patterns of V. kalakhensis and V. eristalioides were compared with that of V. narbonensis which is considered to be the ancestor of the Narbonensis complex. The results indicate that qualitative and quantitative flavonoid data may be used in the study of the organization and evolution of the Narbonensis complex.