Elizabeth A. Widjaja
Indonesian Institute of Sciences
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Featured researches published by Elizabeth A. Widjaja.
Bioorganic & Medicinal Chemistry | 2013
Tyler A. Johnson; Johann Sohn; Aidan E. Ward; Tanya L. Cohen; Nicholas Lorig-Roach; Haixia Chen; Ronaldo Aloise Pilli; Elizabeth A. Widjaja; Muhammad Hanafi; Leonardus B S Kardono; Puspa D. Lotulung; Kyria Boundy-Mills; Leonard F. Bjeldanes
An evaluation of Indonesian plants to identify compounds with immune modulating activity revealed that the methanolic extract of an Alphonsea javanica Scheff specimen possessed selective anti-inflammatory activity in a nuclear factor-kappa B (NF-κB) luciferase and MTT assay using transfected macrophage immune (Raw264.7) cells. A high-throughput LC/MS-ELSD based library approach of the extract in combination with the NF-κB and MTT assays revealed the styryl lactone (+)-altholactone (2) was responsible for the activity. Compound 2, its acetylated derivate (+)-3-O-acetylaltholactone (3), and the major compound of this class, (+)-goniothalmin (1), were further evaluated to determine their anti-inflammatory potential in the NF-κB assay. Concentration-response studies of 1-3 indicated that only 2 possessed NF-κB based anti-inflammatory activity. Compound 2 reduced the LPS-induced NO production, phosphorylation of IκBα, and the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) using Western blot analysis. Further studies using qPCR indicated 2 reduced the expression of eight pro-inflammatory cytokines/enzymes (0.8-5.0μM) which included: COX-2, iNOS, IP-10, IL-1β, MCP-1, GCS-F, IL-6 and IFN-β. These results indicated that 2 displays broad spectrum immune modulating activity by functioning as an anti-inflammatory agent against LPS-induced NF-κB signaling. Conversely the selective cytotoxicity and in vivo anti-tumor and anti-inflammatory activity previously reported for 1 do not appear to arise from a mechanism that is linked to the NF-κB immune mediated pathway.
Reinwardtia | 2014
Elizabeth A. Widjaja; R.N. Lester
Gigantochloa pseudoarundinacea, G. robusta, G. atter, G. atroviola-cea and 14 other related Malesian species of bamboos were studied from preserved and living materials. Characters discerned by morphology, anatomy, chromatography and electrophoresis were analysed by numerical taxonomy. Most of the data indicated that the four species named above were distinct although closely related. The relationships of the remaining species were more complex, but the integrity of each species was established.
Reinwardtia | 1997
Elizabeth A. Widjaja
Reinwardtia | 2014
Elizabeth A. Widjaja
Reinwardtia | 2014
Elizabeth A. Widjaja; R.N. Lester
Reinwardtia | 2003
Elizabeth A. Widjaja; Inggit Pudji Astuti; Ida Bagus Ketut Arinasa
Reinwardtia | 2009
Elizabeth A. Widjaja; Nursahara Pasaribu; Arief Hidayat
BERITA BIOLOGI | 2006
Elizabeth A. Widjaja
Reinwardtia | 2018
Yeni Rahayu; Tatik Chikamawati; Elizabeth A. Widjaja
The Gardens' Bulletin, Singapore | 2017
I Putu Gede P. Damayanto; Elizabeth A. Widjaja; Present address Rt; Rw