Elvira Rea

Yield, fruit quality and mineral composition of grafted melon plants grown under saline conditions

Summary In many irrigated areas of the Mediterranean region, farmers are forced to use saline water to irrigate their crops due to an inadequate supply of fresh water. Grafting may represent an effective tool to improve crop tolerance to salinity. Two greenhouse experiments were carried out to determine yield, fruit quality and mineral composition of melon plants (Cucumis melo L. cv. ‘Cyrano’), either ungrafted or grafted onto the commercial rootstock ‘P360’ (Cucurbita maxima C. moschata) and cultured in pumice. Plants were supplied with nutrient solutions having electrical conductivities (EC) of 2.0, 4.0, 5.9, 7.8 or 9.7 dS m–1. The saline nutrient solutions (those > 2.0 dS m–1) had the same basic composition, plus an additional 20, 40, 60 or 80 mM NaCl, respectively. In both years (2003 and 2004), increased salinity in the nutrient solution resulted in a linear decrease in marketable yield compared to controls that was due to reductions in the size and number of marketable fruits. Averaged across years and nutrient solution concentrations, marketable fruit yield was 44% higher in grafted than in ungrafted plants. The lowest marketable yield recorded in ungrafted plants was associated with a reduction in both mean fruit weight and in the number of fruits per plant compared to grafted plants. Salinity improved fruit quality in both grafting combinations by increasing firmness, dry matter (DM), acidity and total soluble solids (TSS) contents. The nutritional qualities of grafted melons such as fruit DM, titratable acidity and TSS content were slightly inferior compared to those of ungrafted plants, whereas physical qualities such as fruit firmness and Hunter colour values (L* and a*/b*) were superior compared to those of plants grown with their own roots. Grafted plants could reduce leaf Na+ ion, but not Cl– ion, concentrations. However, the sensitivity to salinity was similar between grafted and ungrafted plants, and the higher marketable yield from grafted plants was mainly due to grafting per se.

Increased antioxidative capacity in maize calli during and after oxidative stress induced by a long lead treatment

Maize (Zea mays L., cv. Samodek) callus cultures were exposed for long period (22 months) to lead (0.5 mM lead chloride) and lead content, oxidative damage and antioxidative response were evaluated at different steps. Inductively coupled plasma (ICP) emission spectroscopy analysis showed that lead entered the cells and it accumulated, but its internal concentration was maintained 10-fold less than the external one. Increase of both polyamine and lipid peroxide content indicated that cells underwent a stress condition due to an oxidative attack, counteracted by an increase of antioxidative defence enzyme activities, ascorbate peroxidase (APX, EC 1.11.1.11) and glutathione reductase (GR, EC 1.6.4.2). After 10 months, from the start of the lead treatment, a stock of calli was transferred for 6 months in a lead-deprived medium and then re-exposed to lead for a further 6 months. Analysis indicated that lead-deprived calli maintained high levels of APX and GR activities, suggesting that, over the experimental time–course, cells with high APX and GR activity were selected and allowed to enrich the cultures. These cultures, after a new lead treatment, showed a lower oxidative damage compared to continuously lead-treated calli.

Improving melon and cucumber photosynthetic activity, mineral composition, and growth performance under salinity stress by grafting onto Cucurbita hybrid rootstocks

The aim of the current work was to determine whether grafting could improve salinity tolerance of melon and cucumber, and whether possible induction of tolerance to salt stress was associated with the protection of the photosynthetic apparatus. Two greenhouse experiments were carried out to determine gas exchange, mineral composition, growth and yield of melon (Cucumis melo L. cv. Cyrano) and cucumber (Cucumis sativus L. cv. Akito) plants, either ungrafted or grafted onto the Cucurbita hybrid rootstocks (Cucurbita maxima Duch. × Cucurbita moschata Duch.), ‘P360’, and ‘PS1313’, respectively. Plants were grown hydroponically and supplied with two nutrient solutions — a nonsalinized control and a salinized solution which contained 40 mmol L−1 of NaCl. Salinity induced a smaller decrease in leaf area index (LAI), in grafted-compared to ungrafted plants. Similarly, the PN and gs reduction in NaCl treatment compared to control were significantly lower in grafted plants (34% and 34%, respectively, for melon and 14% and 15.5%, respectively, for cucumber) compared to ungrafted plants (42% and 40%, respectively, for melon and 30% and 21%, respectively, for cucumber). In all grafting combinations, negative correlations were recorded between Na+ and Cl− in the leaf tissue and PN. Grafting reduced concentrations of sodium, but not chloride, in leaves. Under saline conditions a smaller reduction in melon and cucumber shoot biomass dry mass and fruit yield were recorded, with positive correlations between shoot biomass, yield and PN. These results suggest that the use of salt tolerant Cucurbita rootstock can improve melon and cucumber photosynthetic capacity under salt stress and consequently crop performance.

Yield, water requirement, nutrient uptake and fruit quality of zucchini squash grown in soil and closed soilless culture

Summary The effects of soil and closed soilless systems (cocofibre, perlite and pumice culture) on precocity, productivity, water use, plant mineral composition and fruit quality were studied on zucchini plants (Cucurbita pepo L. ‘Afrodite’ grown in a greenhouse at Viterbo, central Italy. The plants grown in a soilless system exhibited higher yield (total, marketable and fruit number), harvest index, and water-use efficiency compared with those grown in soil. Among soilless treatments, pumice had the lowest water requirement and hence the highest water use efficiency. Use of cocofibre led to the earliest yield compared with the other substrates due probably to the higher minimal temperatures recorded on the organic substrate. Plants grown in soilless culture had a higher uptake of N, Mg, Na, Fe, Cu, Mn, Zn than those grown in soil. After 73 d of solution recycling in soilless treatments, N-NO3, K, P, Fe and Mn were depleted by 26, 16, 40, 92, and 25% respectively, while Ca, Mg, Na, Cu and Zn increased by 6, 69, 113, 360, and 981 % respectively. Carbohydrate concentration (glucose, fructose, sucrose and starch) increased in soilless over soil culture. No significant differences were observed in dry matter or total protein content. Nitrate concentration of fruits was lower in soil than in soilless treatments. The results demonstrated that the growers may improve water and nutrient control, yield and fruit quality by switching from soil to closed soilless culture.

Nursery growing media: agronomic and environmental quality assessment of sewage sludge-based compost

There is a stringent need to reduce the environmental impact of peat in the plant nursery production chain. In this experiment, the use of different rates of sewage sludge compost in the preparation of growing media for potted Bougainvillea was evaluated to assess its efficiency for the replacement of peat and to quantify the environmental impact of such alternative substrates by the life cycle assessment (LCA) method. Five substrates containing increasing proportion of composted sewage sludge to peat (0%, 25%, 40%, 55%, and 70% v/v) were used, and their physicochemical properties were measured. Bougainvillea plant growth, biomass production, and macro- and micronutrient absorption were also determined. The main results were that compost addition improved the plant nutrient and increased the substrate pH, electrical conductivity (EC), and dry bulk density values. Globally, the results showed that compost could be used at up to 55% by volume with no negative effects on plant growth. The LCA showed that use of compost reduced the environmental loads of the growth media, except the Global Warming Potential value (GWP100). Environmental implications of the use of compost in the plant nursery chain are discussed.

compost-based nursery substrates: effect of peat substitution on organic melon seedlings

A bovine manure compost (BMC) and a green compost (GC) were evaluated as components of substrates in partial substitution of peat for organic melon seedlings production. Treatments, differentiated on the basis of the volume percentage of the two analysed composts, were compared to the control (a mixture of peat, coconut fibre and perlite). In order to respect the guidelines of organic production, all the treatments were fertilised, at the beginning of the trial, with guano. Compost-based substrates were analysed for physical, physicochemical and chemical parameters (bulk density, porosity, pH, EC, nutrients content etc.). Even though significant differences were observed, recorded values were in the range of acceptability for growing media. In terms of performance, seedling growth in treatments containing 30% and 50% of composts was significantly higher than in control.

EFFECT OF PEAT-REDUCED AND PEAT-FREE SUBSTRATES ON ROSEMARY GROWTH

The objective of this work was to study the use of four composts, obtained by agro-industrial, urban and green wastes, as growing media components on Rosmarinus officinalis L. Substrates were obtained by mixing each compost with peat in different proportions. Main physical and chemical characteristics of prepared substrates have been compared and, at the end of growing cycle, the biometric survey on main growing parameters and plant nutritional status was performed. The obtained results showed that substrates with 30% compost have main physical and chemical parameters comparable with those of the control. Best quality plants have been obtained substituting peat with 30% of compost, except with the olive mill compost. At the end, the green pruning compost can be recommended as growing media component (up to 50%) for the growth of Rosmarinus officinalis L., being able to determine high quality plants, together with an implemented plant nutrient efficiency.

Inhibition of maize primary root elongation by spermidine: Effect on cell shape and mitotic index

Spermidine applied for 18 h to intact maize seedlings through their roots reduces root growth 70%, and the effect is reversible. Histological observations of longitudinal sections of 0.4-cm root apical segments from 2-day-old maize seedlings grown for 18 h in 0.5 m CaSO4 solution with or without 1 mm spermidine contribute to the explanation of spermidine-dependent slow root growth. In the meristematic zone a strong reduction of the mitotic index and in the elongation zone an inhibition of cell elongation occur simultaneously. Cell shape analysis along the growth axis of the maize root apex expressed in terms of form factor (FCircle) values substantiates the dual effect of spermidine on mitotic activity and cell elongation.

Salinity source-induced changes in yield, mineral composition, phenolic acids and flavonoids in leaves of artichoke and cardoon grown in floating system

BACKGROUND Leaves of artichoke (Cynara cardunculus L. subsp. scolymus (L.) Hegi) and cardoon (Cynara cardunculus L. var. altilis DC) are traditionally used as herbal medicine. Moderate salt stress could enhance antioxidant activity and phytochemicals in leaves. The aim of this study was to evaluate the effect of chloride salts (NaCl, KCl and CaCl2) on biomass production, mineral composition, phenolic and flavonoid contents and antioxidant activity in leaves of artichoke and cardoon grown in a floating system. RESULTS In both crops, NaCl and KCl treatments reduced biomass production, while similar values were recorded in CaCl2 and control treatments. In both crops, KCl treatment enhanced total phenolic and flavonoid contents, antioxidant activity and target polyphenols in leaves harvested at 48, 82 and 105 days after sowing (DAS), while leaf quality was improved by NaCl and CaCl2 treatments only at 82 and 105 DAS. Irrespective of salinity, leaves of cardoon had higher total phenolic and flavonoid contents, antioxidant activity and target polyphenols than those of artichoke. CONCLUSION The results showed that application of KCl can be considered an effective way to produce high-quality leaves of artichoke and cardoon during the whole cropping cycle, although resulting in a 30% reduction in plant biomass.

Graft union formation in artichoke grafting onto wild and cultivated cardoon: An anatomical study

In order to develop a non-chemical method such as grafting effective against well-known artichoke soil borne diseases, an anatomical study of union formation in artichoke grafted onto selected wild and cultivated cardoon rootstocks, both resistant to Verticillium wilt, was performed. The cardoon accessions Belgio (cultivated cardoon) and Sardo (wild cardoon) were selected as rootstocks for grafting combinations with the artichoke cv. Romolo. Grafting experiments were carried out in the autumn and spring. The anatomical investigation of grafting union formation was conducted by scanning electron microscopy (SEM) on the grafting portions at the 3rd, 6th, 10th, 12th day after grafting. For the autumn experiment only, SEM analysis was also performed at 30 d after grafting. A high affinity between artichoke scion and cardoon rootstocks was observed, with some genotype differences in healing time between the two bionts. SEM images of scion/rootstock longitudinal sections revealed the appearance of many interconnecting structures between the two grafting components just 3d after grafting, followed by a vascular rearrangement and a callus development during graft union formation. De novo formation of many plasmodesmata between scion and rootstock confirmed their high compatibility, particularly in the globe artichoke/wild cardoon combination. Moreover, the duration of the early-stage grafting process could be influenced not only by the scion/rootstock compatibility, but also by the seasonal conditions, being favored by lower temperatures and a reduced light/dark photoperiod.