Alessandra Trinchera

Humic substances along the profile of two Typic Haploxerert

Abstract In Vertisols, organic matter contributes to soil colour by formation of organo-mineral complexes and affects morphological, physico-chemical, biological and biochemical properties. Turbation may affect the chemical and structural composition of the most stabilised fractions of soil organic matter (SOM), i.e., humic substances (HS). The objectives of this study were to: (1) characterise SOM in two Vertisols (V1 and V2) developed under Mediterranean climate in Italy, using some HS characteristics as indicators of SOM turnover in Vertisols, and (2) explore possible differences related to the pedomorphologic conditions of the area under which two soils have formed. SOM evolution along the soil profile was investigated by applying different techniques to chemically extracted HS. Humification parameters, thermal methods, such as Differential Scanning Calorimetry (DSC) and Thermogravimetry (TG) together with isoelectric focusing (IEF) were used. The results showed a significant difference in the chemical characteristics of SOM in the considered Vertisols. A higher amount of total and humified carbon was found in pedon V2. In particular, the results indicated an accumulation of humified compounds in deeper horizons of V2: this effect is particularly significant since both soils were under the same agricultural management and the same climatic conditions, but were different in physiografic position, slope and colour. The higher amount of not humified SOM in the pedon V1 could explain the differences found in the shape and size of soil structure between the two pedons. The different SOM evolution occurring in the two Vertisols apparently influenced the structure and composition of the HS fraction. Thermal analysis and isoelectric focusing data provided evidence of a different level of stabilisation of HS from the two Vertisols. The HS from pedon V2 have a higher molecular weight structure, which would imply a higher level of SOM stabilisation. Conversely, HS in the pedon V1 appeared to be richer in aliphatic and phenolic structures, which may imply the presence of less stabilised HS fractions. These findings agree well with the different water dynamics occurring in the two sites, which confirmed the influence exerted by water on chemical and structural composition of HS and, consequently, their effect in determining Vertisols colour. In conclusion, thermal and isoelectric focusing characterisation of HS appear to shed some light in understanding the structural complexity of Vertisols. The results obtained in the present study, if confirmed on a larger number of Vertisols, suggest the possibility of using HS as indicators of SOM turnover in relation to pedomorphology, use and management of soils.

Thermal analysis in the evaluation of compost stability: a comparison with humification parameters

Characterization of organic matter of six composts from agroindustrial wastes was carried out by both chemical analysis and thermal analysis in order to assess their level of stability. Degree of humification (DH%) and index of humification (HI) were calculated after extraction, fractionation and analysis of the organic carbon from composts. Differential Scanning Calorimetry (DSC) and thermogravimetry (TG) were simultaneously performed in oxidizing conditions on whole ground composts. Thermoanalitical data resulted to be useful in integrating quantitative information coming from chemical analysis of humified fraction of compost organic matter. Particularly, DSC curves allow to distinguish between well and poor stabilized composts, and information deriving from weight losses, registered by TG curves, permits to individuate a thermoanalytical parameter (R1) that resulted to be well correlated to humification parameters DH% and HI.

Effect of long term addition of composts and poultry manure on soil quality of citrus orchards in Southern Italy

A 6-year study was conducted in an organically managed orange orchard located in Sicily (Southern Italy) to assess the effect of compost and organic fertiliser utilisation on soil quality. Adopting a randomised-block experimental design with three replicates, four treatments were carried out. In treatments 1 and 2, two different composts (C1 from distillery by-products and C2 from livestock waste) were applied. The plots of treatment 3 were fertilised using dried poultry manure. The control treatment was fertilised by mineral/synthetic fertilisers. In order to verify the hypothesis that composts and organic fertilisers improve soil fertility, soil quality was evaluated by selecting dynamic soil parameters, as indicators linked to C and N cycles. Total organic C, total N, C/N ratio, humified fraction, isoelectric focussing (IEF) of extracted organic matter, microbial biomass C, potentially mineralisable N under anaerobic conditions, potentially mineralisable C, C mineralisation quotient and metabolic quotient were determined for each sample. Moreover, the Community Level Physiological Profile (by Biolog technique) was defined, calculating derived functional biodiversity and versatility indexes. Parameters related to IEF and potentially mineralisable C showed significant differences among the treatments. Moreover, total C, total N and humification parameters tended to increase, while no differences were observed in biodiversity indexes. On these findings, it was concluded that composts and poultry manure only weakly affected soil properties, though they increased soil potentially available nutritive elements to crops.

compost-based nursery substrates: effect of peat substitution on organic melon seedlings

A bovine manure compost (BMC) and a green compost (GC) were evaluated as components of substrates in partial substitution of peat for organic melon seedlings production. Treatments, differentiated on the basis of the volume percentage of the two analysed composts, were compared to the control (a mixture of peat, coconut fibre and perlite). In order to respect the guidelines of organic production, all the treatments were fertilised, at the beginning of the trial, with guano. Compost-based substrates were analysed for physical, physicochemical and chemical parameters (bulk density, porosity, pH, EC, nutrients content etc.). Even though significant differences were observed, recorded values were in the range of acceptability for growing media. In terms of performance, seedling growth in treatments containing 30% and 50% of composts was significantly higher than in control.

EFFECT OF PEAT-REDUCED AND PEAT-FREE SUBSTRATES ON ROSEMARY GROWTH

The objective of this work was to study the use of four composts, obtained by agro-industrial, urban and green wastes, as growing media components on Rosmarinus officinalis L. Substrates were obtained by mixing each compost with peat in different proportions. Main physical and chemical characteristics of prepared substrates have been compared and, at the end of growing cycle, the biometric survey on main growing parameters and plant nutritional status was performed. The obtained results showed that substrates with 30% compost have main physical and chemical parameters comparable with those of the control. Best quality plants have been obtained substituting peat with 30% of compost, except with the olive mill compost. At the end, the green pruning compost can be recommended as growing media component (up to 50%) for the growth of Rosmarinus officinalis L., being able to determine high quality plants, together with an implemented plant nutrient efficiency.

Use of biochemical indexes and changes in organic matter dynamics in a Mediterranean environment: a comparison between soils under arable and set-aside managements

Abstract The aims of this study were to evaluate the influence of different agronomic practices on organic matter turnover and on microbiological activity of two soils of the Castelporziano Tenuta Reserve. The first of the two soils is arable, currently cultivated with oats, while the second is a set-aside soil which has been semi-reforested in the last ten years. Results revealed that set-aside soil was characterised by slower organic carbon turnover and better humification efficiency with respect to the arable soil. This study confirms that the restoration practice can establish a self-sustaining plant and microrganisms community, which is naturally evolving in the direction of the original, stable ecosystem.

Graft union formation in artichoke grafting onto wild and cultivated cardoon: An anatomical study

In order to develop a non-chemical method such as grafting effective against well-known artichoke soil borne diseases, an anatomical study of union formation in artichoke grafted onto selected wild and cultivated cardoon rootstocks, both resistant to Verticillium wilt, was performed. The cardoon accessions Belgio (cultivated cardoon) and Sardo (wild cardoon) were selected as rootstocks for grafting combinations with the artichoke cv. Romolo. Grafting experiments were carried out in the autumn and spring. The anatomical investigation of grafting union formation was conducted by scanning electron microscopy (SEM) on the grafting portions at the 3rd, 6th, 10th, 12th day after grafting. For the autumn experiment only, SEM analysis was also performed at 30 d after grafting. A high affinity between artichoke scion and cardoon rootstocks was observed, with some genotype differences in healing time between the two bionts. SEM images of scion/rootstock longitudinal sections revealed the appearance of many interconnecting structures between the two grafting components just 3d after grafting, followed by a vascular rearrangement and a callus development during graft union formation. De novo formation of many plasmodesmata between scion and rootstock confirmed their high compatibility, particularly in the globe artichoke/wild cardoon combination. Moreover, the duration of the early-stage grafting process could be influenced not only by the scion/rootstock compatibility, but also by the seasonal conditions, being favored by lower temperatures and a reduced light/dark photoperiod.

Study of Organic Matter Evolution in Citrus Compost by Isoelectrofocusing Technique

Three composts from citrus-processing industry wastes, sampled at prefixed times during the composting process, were studied with the aim to follow the organic matter evolution by using the isoelectrofocusing (IEF) technique. Results indicated that IEF qualitative analyses allowed evaluation of the organic matter transformation during composting process, showing a decrease of IEF peaks focused at lower values of pH (less stabilized organic matter) and a corresponding increase of peaks focused at higher value of pH (more humified material). The parameter A %, defined as the areas sum of IEF peaks focused at pH>4.7, could be considered particularly effective as a “threshold value” to evaluate the level of organic matter evolution for the considered composts.

Ecological service providing crops effect on melon-weed competition and allelopathic interactions

Cover crops provide a wide range of ecological benefits, so to be defined as Ecological Service providing Crops (ESCs). Accordingly, different ESCs could influence both weed density and biomass due to: (i) allelopathic effects, (ii) increased cash crop-weed competitiveness. In order to test this hypothesis, a field experiment was carried out in Central Italy, comparing different autumn-winter cereal ESCs (wheat, barley, spelt, rye, and their mixture), preceding melon crop, with a control (no ESC). Weed performances were evaluated during both the ESC and melon cropping cycles. At flowering stage, the ESCs were flattened by a roller crimper, obtaining a mulch layer in which the melon was transplanted. The competitive weed-crop relationship was assessed by means of indices of competition. Bioassay tests were performed to evaluate the allelopathic potential of the ESCs on target weed. The results obtained from both the open field and the laboratory tests demonstrated that the different ESC species significantly affected weed density before and after flattening, although no difference on ESC biomasses was recorded at termination. At melon harvest, weed biomass was significantly lower in ESC treatments than in control one, and crop biomasses did not show any difference among all treatments. Nevertheless, the melon competitive ability was significantly higher in the rye and barley mulch compared to control. The bioassay test showed lower root germination and growth in the tested ESC extracts with respect to the control. By this, the ESC species showed a role in weed management both during their cycle and after termination, giving a competitive advantage to the cash crop, probably due also to active allelopathic compounds released by the ESCs.

Nutritional response of Zea mays L. seedlings to “on plant demand” fertilizer: A vector analysis approach

ABSTRACT Glass matrix based fertilizers (GMF) are new mineral fertilizers, able to release nutrients on the basis of plant demand: they are soluble only in metal complexing solutions, such as those exuded by plant roots. Moreover, nutrient release could be increased by mixing the glass-matrix fertilizer to different organic biomasses, such as leather meal, digested vine vinasse, etc. A greenhouse pot experiment was conducted for assessing the effect of an organo-mineral fertilizer, obtained by mixing GMF with increasing percentages of digested vine vinasse [DVV, at 5% (GMF95), 20% (GMF80) and 50% (GMF50) w/w], on the root and shoot biomass and nutritional status of Zea mays L. seedlings. Among tested fertilizers, the formulations GMF80 and GMF50 gave the best results as shoot/root ratio. The treatments with GMF, GMF95 and GMF80 gave rise to a significant increase of both shoot and root biomass and a tendency to increase the macro and micronutrients availability.