Emanuele Del Guacchio

New alien vascular species for the flora of southern Italy

Abstract In the present work new or confirmed alien not-cultivated vascular plants are reported for the flora of the southern Italy regions: Acalypha virginica, Alcea biennis subsp. biennis, Bidens subalternans, Cardamine occulta, Casuarina equisetifolia, Cedrus atlantica, Cedrus deodara, Chamaeiris orientalis, Cotula coronopifolia, Elodea canadensis, Eragrostis mexicana subsp. virescens, Euphorbia nutans, Fagopyrum esculentum, Ficus microcarpa, Hesperocyparis arizonica, Hesperocyparis glabra, Kalanchoë × houghtonii, Lantana camara subsp. aculeata, Lemna minuta, Ligustrum sinense, Lobelia erinus, Lonicera japonica, Oenothera lindheimeri, Panicum capillare, Persicaria capitata, Phyla nodiflora, Schinus molle, Sedum palmeri, Sesbania punicea, Solanum elaeagnifolium, Solanum sisymbriifolium and Veronica peregrina. Furthermore, Lantana montevidensis is new for the Italian alien flora, Asparagus asparagoides and Tradescantia pallida are reported for the first time in continental Italy, whereas Cucurbita moschata, Nandina domestica and Taxodium distichum are new for the Italian peninsula. Moreover, field surveys in Campania allow us to update at regional level the naturalization status of Euphorbia nutans (naturalized) and Ligustrum sinense (casual). The new data confirm that new non-native species are continuously introduced into the flora of southern Italy and it is noteworthy that several species, widely used in the reforestation since the middle of the last century, have undergone naturalization in recent years.

What is in your cup of tea? DNA Verity Test to characterize black and green commercial teas

In this study, we used several molecular techniques to develop a fast and reliable protocol (DNA Verity Test, DVT) for the characterization and confirmation of the species or taxa present in herbal infusions. As a model plant for this protocol, Camellia sinensis, a traditional tea plant, was selected due to the following reasons: its historical popularity as a (healthy) beverage, its high selling value, the importation of barely recognizable raw product (i.e., crushed), and the scarcity of studies concerning adulterants or contamination. The DNA Verity Test includes both the sequencing of DNA barcoding markers and genotyping of labeled-PCR DNA barcoding fragments for each sample analyzed. This protocol (DVT) was successively applied to verify the authenticity of 32 commercial teas (simple or admixture), and the main results can be summarized as follows: (1) the DVT protocol is suitable to detect adulteration in tea matrices (contaminations or absence of certified ingredients), and the method can be exported for the study of other similar systems; (2) based on the BLAST analysis of the sequences of rbcL+matK±rps7-trnV(GAC) chloroplast markers, C. sinensis can be taxonomically characterized; (3) rps7-trnV(GAC) can be employed to discriminate C. sinensis from C. pubicosta; (4) ITS2 is not an ideal DNA barcode for tea samples, reflecting potential incomplete lineage sorting and hybridization/introgression phenomena in C. sinensis taxa; (5) the genotyping approach is an easy, inexpensive and rapid pre-screening method to detect anomalies in the tea templates using the trnH(GUG)-psbA barcoding marker; (6) two herbal companies provided no authentic products with a contaminant or without some of the listed ingredients; and (7) the leaf matrices present in some teabags could be constituted using an admixture of different C. sinensis haplotypes and/or allied species (C. pubicosta).

Transfer of Four Species of Scabiosa to Lomelosia (Dipsacaceae)

Abstract.  Four species of Scabiosa L. are transferred to Lomelosia Raf. (Dipsacaceae) to complete the relocation of all scabious taxa with a pitted epicalyx into Lomelosia. The new combinations are: L. deserticola (Rech. f.) P. Caputo & Del Guacchio, L. poecilocarpa (Rech. f.) P. Caputo & Del Guacchio, L. schimperiana (Boiss. & Buhse) P. Caputo & Del Guacchio, and L. transcaspica (Rech. f.) P. Caputo & Del Guacchio.

The Linnaean names in Statice (Plumbaginaceae)

The Linnaean names Statice aurea, S. cordata, S. flexuosa, S. incana, S. pruinosa, and S. speciosa (Plumbaginaceae) are investigated. Specimens from the Linnaean Herbarium (LINN) are designated as lectotypes of S. aurea, S. flexuosa, S. pruinosa and S. speciosa, and an illustration is designated as lectotype for S. cordata. Finally, the typification of the remaining Linnaean names in Statice is briefly reviewed and discussed.

DNA Barcoding to Confirm the Morphological Identification of the Coral Trees (Erythrina spp., Fabaceae) in the Ancient Gardens of Naples (Campania, Italy)

The coral trees (genus Erythrina) have been fostering great interest among the botanists and gardeners of Naples, since their arrival in Europe in the second half of the 18th century. Numerous species were present in the royal and private botanical gardens of the region, but their number has decreased today. The purpose of this work was to verify which species occur nowadays in the public areas of Naples and associate them with the historical information about their introduction. The identification was carried out also by molecular methods, by means of sequencing nuclear and chloroplast DNA markers. The comparison of the sequences obtained for the specimens present in Naples with those present in the literature, together with a morphological examination, allowed us to identify with accuracy the species anciently introduced or nowadays cultivated in Naples.

Correction: What is in your cup of tea? DNA Verity Test to characterize black and green commercial teas

[This corrects the article DOI: 10.1371/journal.pone.0178262.].