Emanuele Sanna

Blood and hair lead levels in boys and girls living in two Sardinian towns at different risks of lead pollution.

This study reports blood and hair lead levels measured in 1998 in 222 children from two Sardinian towns: Portoscuso and Sestu. Portoscuso is in a polluted area of Sardinia due to its vicinity to the industrial zone of Portovesme. As a consequence of its economy and location, Sestu is not exposed to lead pollution. Blood lead (PbB) concentration was determined in heparinized venous blood samples by graphite furnace atomic absorption spectrophotometry (AAS). Hair lead (PbH) concentration was determined by inductively coupled plasma atomic emission spectrometry (ICP-AES). With respect to blood lead levels, the boys of Portoscuso have the highest arithmetic mean value (11.30 microg/dL), followed by the Portoscuso girls (7.39 microg/dL); they are followed, but with much lower values, by the boys (4.09 microg/dL) and girls (3.34 microg/dL) of Setsu. For hair lead levels, the Portoscuso boys have the highest arithmetic mean value (15.51 microg/g), followed by the Portoscuso girls (8.82 microg/g) and the Sestu boys (4.03 microg/g) and girls (2.83 microg/g). Therefore the mean values of PbB and PbH follow similar patterns in the boys and girls of the two Sardinian towns. Two-way ANOVA reveals a significant effect of sex and town on log PbB and log PbH. Moreover, values of the Bravais-Pearson coefficients of correlation between log PbB and log PbH are statistically significant for the total sample (r=0.5086; P<0.001), for males (r=0.4275; P<0.01), and for females (r=0.4859, P<0.001). The sensitivity of the hair lead analysis in identifying lead concentrations above 10 microg/g is 49%. The results support the hypothesis that hair lead levels can be considered an indicator of different relative exposure of populations to lead pollution.

Anthropometric changes in urban Sardinian children 7 to 10 years between 1975-1976 and 1996.

This study is a comparison of several anthropometric variables in school children of the same age (7–10 years) and sex measured in two primary schools in Cagliari (Sardinia, Italy) at an interval of 20 years (1996 vs. 1975–1976). For both males and females of each age‐class, there are significant differences in the following dimensions: weight, stature, estimated lower limb length, biacromial breadth, xiphoid chest circumference, xiphoid chest depth, and bicristal breadth, and also in relative sitting height, cephalic index, and facial index. The means of the relative sitting height, cephalic, and facial indexes are higher in the children measured in 1975–1976 than in those measured in 1996. There are no significant differences in sitting height and arm length. In addition, the estimated BMIs of the 1975–1976 sample are, on average, lower than those of the 1996 sample. The results suggest that urban Sardinian children sampled in 1996 are generally taller, heavier, and larger and have a longer head and wider face than their peers of 20 years earlier. The increase in stature is due more to an increase in lower limb length than in sitting height. Am J. Hum. Biol. 12:782–791, 2000.

Mine, Yours, Ours? Sharing Data on Human Genetic Variation

The achievement of a robust, effective and responsible form of data sharing is currently regarded as a priority for biological and bio-medical research. Empirical evaluations of data sharing may be regarded as an indispensable first step in the identification of critical aspects and the development of strategies aimed at increasing availability of research data for the scientific community as a whole. Research concerning human genetic variation represents a potential forerunner in the establishment of widespread sharing of primary datasets. However, no specific analysis has been conducted to date in order to ascertain whether the sharing of primary datasets is common-practice in this research field. To this aim, we analyzed a total of 543 mitochondrial and Y chromosomal datasets reported in 508 papers indexed in the Pubmed database from 2008 to 2011. A substantial portion of datasets (21.9%) was found to have been withheld, while neither strong editorial policies nor high impact factor proved to be effective in increasing the sharing rate beyond the current figure of 80.5%. Disaggregating datasets for research fields, we could observe a substantially lower sharing in medical than evolutionary and forensic genetics, more evident for whole mtDNA sequences (15.0% vs 99.6%). The low rate of positive responses to e-mail requests sent to corresponding authors of withheld datasets (28.6%) suggests that sharing should be regarded as a prerequisite for final paper acceptance, while making authors deposit their results in open online databases which provide data quality control seems to provide the best-practice standard. Finally, we estimated that 29.8% to 32.9% of total resources are used to generate withheld datasets, implying that an important portion of research funding does not produce shared knowledge. By making the scientific community and the public aware of this important aspect, we may help popularize a more effective culture of data sharing.

Craniofacial morphometric variation and the biological history of the peopling of Sardinia

The aim of this work is to explore the pattern of craniofacial morphometric variation and the relationships among five prehistoric Sardinian groups dated from Late Neolithic to the Nuragic Period (Middle and Late Bronze Age), in order to formulate hypotheses on the peopling history of Sardinia. Biological relationships with coeval populations of central peninsular Italy were also analysed to detect influences from and towards extra-Sardinian sources. Furthermore, comparison with samples of contemporary populations from Sardinia and from continental Italy provided an indication of the trend leading to the final part of the peopling history. Finally, Upper Palaeolithic and Mesolithic samples were included in the analyses to compare the prehistoric Sardinians with some of their potential continental ancestors. The analysis is based on multivariate techniques including Mahalanobis D(2) distance, non-parametric multidimensional scaling (MDS) and principal component analysis (PCA). The results showed the tendency to progressive differentiation between Sardinian groups and peninsular Italian groups, with the possible exception of a discontinuity showed by the Bonnànaro (Early Bronze Age) Sardinian sample. Several aspects of the morphological results were found to agree with the current genetic evidence available for the present-day Sardinian population and a Nuragic sample: (1) biological divergence between the Sardinian and peninsular Italian populations; (2) similarity/continuity among Neolithic, Bronze Age and recent Sardinians; (3) biological separation between the Nuragic and Etruscan populations; (4) contribution of a Palaeo-Mesolithic gene pool to the genetic structure of current Sardinians.

When data sharing gets close to 100%: what human paleogenetics can teach the Open Science movement

This study analyzes data sharing regarding mitochondrial, Y chromosomal and autosomal polymorphisms in a total of 162 papers on ancient human DNA published between 1988 and 2013. The estimated sharing rate was not far from totality (97.6% ± 2.1%) and substantially higher than observed in other fields of genetic research (evolutionary, medical and forensic genetics). Both a questionnaire-based survey and the examination of Journals’ editorial policies suggest that this high sharing rate cannot be simply explained by the need to comply with stakeholders requests. Most data were made available through body text, but the use of primary databases increased in coincidence with the introduction of complete mitochondrial and next-generation sequencing methods. Our study highlights three important aspects. First, our results imply that researchers’ awareness of the importance of openness and transparency for scientific progress may complement stakeholders’ policies in achieving very high sharing rates. Second, widespread data sharing does not necessarily coincide with a prevalent use of practices which maximize data findability, accessibility, useability and preservation. A detailed look at the different ways in which data are released can be very useful to detect failures to adopt the best sharing modalities and understand how to correct them. Third and finally, the case of human paleogenetics tells us that a widespread awareness of the importance of Open Science may be important to build reliable scientific practices even in the presence of complex experimental challenges.

Adipose tissue: Another target organ for lead accumulation? A study on sardinian children (Italy)

This study analyzes the relationship between lead levels and adipose tissue in Sardinian children. As an environmental pollutant, lead occurs in two different chemical forms: inorganic and organic lead; organic lead is present in the environment mainly as lead‐alkyls (tetramethyl and tetraethyl lead). The lead‐alkyls are characterized by their liposolubility; because of this characteristic, the behavior of organic Pb in the organism is similar to that of persistent organic pollutants (POPs). In fact, the high liposolubility of POPs, synthetic chemical substances highly resistant to biodegradation in both the environment and the human body, gives them a preferential tendency to accumulate in adipose tissue.

Are changes in body dimensions of adult females from Italy (Sardinia and Latium) related to secular trend

This paper presents secular changes in height, weight, sitting height, relative sitting height, BMI and estimated lower limb length in two samples of Italian adult females from Sardinia (Cagliari) and Latium (Rieti). The samples consist of 579 healthy women from the province of Cagliari and 138 from the town of Rieti, aged 20.0-39.9 years, measured in the period 2003-2006. The women were divided into four 5-year age groups. The anthropometric variables were considered according to different socioeconomic status (SES) in the Cagliari sample, while the Rieti sample was considered as a whole, as the SES was homogeneous. ANOVA results suggest that the secular trend was very slow or had come to a halt in the Rieti sample but continues in the Cagliari sample, as shown by the statistically significant differences for estimated lower limb length (p<or=0.02), and relative sitting height (p<or=0.05). However, these differences disappear after ANCOVA with sibship size and socioeconomic status controlled for, suggesting that they depend mainly on the composition of the Cagliari sample in terms of SES. Therefore, it can be hypothesised that the secular trend might be very slow or has stopped in the Cagliari subsamples homogeneous for SES.

Changes in body and head dimensions in urban Sardinian children (3-5 years) from 1986 to 2001.

Primary objectives : The study describes and discusses the changes in body and head dimensions of urban pre-school children from Sardinia in a 15-year period. Methodology : The mean values of 11 anthropometric variables measured in 2001 in 414 children (209 males, 205 females) from 3 to 5 years old were compared with those measured in 1986 in 262 coeval children (131 males, 131 females). The t -test was used to evaluate whether the differences between the two samples, divided by sex, were statistically significant. Results : For each age class and sex, the differences between the means were significant for the following anthropometric variables: weight, stature, estimated lower limb length, biacromial breadth, bicristal breadth, body mass index, relative sitting height index, head length, and cephalic index. In contrast, the differences between the means were not significant for sitting height and head breadth. Conclusions : The pre-school children of Cagliari measured in 2001 were on average taller and especially heavier than their same-sex peers measured in 1986; the increase in height was clearly due to the increased length of the lower limbs. The transverse dimensions of the trunk also increased, while its length remained largely the same. Head length also increased, while the relative sitting height and cephalic indexes decreased.

Palmar pattern frequencies as indicators of relationships among Sardinian linguistic groups of males

AbstractPalmar pattern frequencies were used to calculate distance coefficients between Sardinian linguistic groups of males for the purpose of verifying, by means of correlation matrix analyses, whether or not the dermatoglyphic traits considered lead to a reliable identification of the biological relationships on the basis of the linguistic backgrounds of these groups. With Sanghvis