Fabrizio Rufo

Samples and data accessibility in research biobanks: an explorative survey

Biobanks, which contain human biological samples and/or data, provide a crucial contribution to the progress of biomedical research. However, the effective and efficient use of biobank resources depends on their accessibility. In fact, making bio-resources promptly accessible to everybody may increase the benefits for society. Furthermore, optimizing their use and ensuring their quality will promote scientific creativity and, in general, contribute to the progress of bio-medical research. Although this has become a rather common belief, several laboratories are still secretive and continue to withhold samples and data. In this study, we conducted a questionnaire-based survey in order to investigate sample and data accessibility in research biobanks operating all over the world. The survey involved a total of 46 biobanks. Most of them gave permission to access their samples (95.7%) and data (85.4%), but free and unconditioned accessibility seemed not to be common practice. The analysis of the guidelines regarding the accessibility to resources of the biobanks that responded to the survey highlights three issues: (i) the request for applicants to explain what they would like to do with the resources requested; (ii) the role of funding, public or private, in the establishment of fruitful collaborations between biobanks and research labs; (iii) the request of co-authorship in order to give access to their data. These results suggest that economic and academic aspects are involved in determining the extent of sample and data sharing stored in biobanks. As a second step of this study, we investigated the reasons behind the high diversity of requirements to access biobank resources. The analysis of informative answers suggested that the different modalities of resource accessibility seem to be largely influenced by both social context and legislation of the countries where the biobanks operate.

Morphometric distances among five ethnic groups and evaluation of the secular trend in historical Libya.

This study analysed the variations, both in space and time, of 10 body dimensions and 2 anthropometric indexes of 745 adult males belonging to 5 ethnic groups of historical Lybia (el-Haràbi, el-Baraghìts, Marabtìn, Oases inhabitants and Tuareg). The data were collected in the years 1928 and 1932 by Puccioni and Cipriani, two Italian anthropologists. The aim was to reconstruct the biological history of Libya at the time, and thus contribute to the ongoing debate on the evolution of the biological standard of living in developing Countries. The subjects were analysed by ethnicity and by 10-year age groups, after adjusting for age. The results of ANCOVA and Tukeys post-hoc test show that among and between groups there are statistical significant differences overall for armspan, height, head breadth, bizygomatic breadth, biiliac breadth/height and head breadth/head length indexes. By means of the cluster analysis, the el-Haràbi, el-Baraghìts and Marabtìn groups cluster together, whereas the Tuareg and Oases inhabitants cluster separately one from the other and both from the other three ethnic groups. Within-group variations are not very marked in all ethnicities. In general, there is the tendency, not statistically significant, to the reduction and/or stasis of body dimensions from the older to the younger, and the differences are greater among the older than the younger age classes. In conclusion, it can be argued that these groups, all different culturally and geographically, were following the same tendency of stasis of the secular trend of the body dimensions considered in this study, and such stasis persisted since, at least, the last twenty years of the 19th century, when the older were born.

Knowledge of evolution and human diversity: a study among high school students of Rome, Italy

BackgroundThis research investigated the knowledge of the complex concept of evolution in a sample (n=1108) of final-year high school students of Rome. Particular attention was given to the evolution of Homo sapiens and to human diversity at the biological and cultural level. Obtained results were analysed in relation to the socio-cultural context of the students. The final objective of the research is to provide teachers, curriculum developers and policy makers with results on basic knowledge on evolution and human diversity of students who are to face the University.MethodsThe research was conducted using an ad hoc questionnaire in five scientific (Liceo scientifico) and four humanistic (Liceo classico) high schools of Rome. The research involved the final-year students, those who are supposed to have a global basic knowledge of cultural and biological aspects of the evolutionary theory. The research project, its aims and modes of realisation were presented and discussed in detail with Deans, teachers and students of the Institutions that volunteered to participate.ResultsThe results show: (1) good knowledge and substantial acceptance of the evolutionary perspective; (2) that cultural and biological diversity are considered as decisive factors in modelling the present-day differences between human groups; (3) that, nonetheless, more than half the students still hold to a classificatory conception of human populations; (4) that the family cultural background is significantly relevant in the education of children.ConclusionsResults of the research highlight some useful recommendations that should contribute to the work of teachers, curriculum developers and policy makers as they refer to what students have learned about evolution and human diversity. These results confirm the fundamental importance of investment in education.

Microgeographic Differentiation in Historical Yemen Inferred by Morphometric Distances

Abstract This study analyzed the variations in space of 8 body dimensions and 11 measures of the head of 1,244 adult Yemenite males, collected in 1933/34 by Coon in Yemen and in Hadhramawt. The aim was to evaluate the presence of geographic microdifferentiation of the populations settled in the different regions of Yemen at the time. Coon sub-divided the sample into six geographical areas according to birthplace and ethnicity of the individuals: Tihamah, the Western Mountains, the Central Plateau, the South Coast, the Eastern Mountains, and Hadhramawt. The results of ANCOVA (age as covariate) show that the observed differences of all variables among the six groups were highly statistically significant. Tukeys post-hoc test reveals higher statistically significant differences among four main groups: (1) Tihamah; (2) the Western Mountains and Central Plateau; (3) the Eastern Mountains; and (4) the Southern Coast and Hadhramawt. Multiple discriminant analysis carried out using only the data of the 11 measures of the head, the more “genetically” determined variables, confirmed these differences. Indeed, the first canonical variate well separates the groups with the Tihamah, Southern Coast and Hadhramawt on the one side and the Eastern Mountains, Western Mountains and Central Plateau on the other. The second canonical variate separates the Tihamah, Western Mountains and Central Plateau from the Eastern Mountains, Southern Coast and Hadhramawt. In conclusion, the Yemenite population seems to be composed of three morphologically distinct groups and an Eastern Mountains group which is positioned between the group formed by the Southern Coast and Hadhramawt and the Western Mountains and Central Plateau group. The Tihamah is the most distant from all the other groups. These differences are probably due to the presence/absence of geographical and cultural barriers that have favored/blocked the gene flow over the years. Indeed, the entire coastal bell, through the centuries, has constituted one of the principal commercial routes between the East, Africa, and the eastern Mediterranean, while the high inland mountains have isolated the remaining communities. This data is also confirmed by genetic studies. Finally, the average height (162.6 cm) of the global Yemenite population, compared to data from the other six middle-eastern Arab countries and Egypt, was found to be 3–6 cm less. This characteristic will be further studied, analyzing variations in average height according to the different age classes in order to evaluate any possible secular changes.

Individual Risk and Collective Fears

If we consider the general concept of risk as a specific cultural modality, we must hold to the view that, as far as medicine is concerned, the concept of risk has followed the historical vicissitudes of those who made its history. In this era of great scientific transformation which has accompanied us over the last few decades, the risk factor has contributed to the greater awareness people have of the limits of modern science. Although medicine has made rapid progress, it has not been able to balance effectively the likelihood of a risk perceived event and the factors that determine the risk itself. Elaborate statistical studies have produced risk estimateswhich do not change the way people perceive the possibility of falling ill. Although society may well ascribe an important role to technology, it has not been able to avoid associating it, in symbolic terms, with the individual and collective risks it has constructed.