Emmanuelle Trevisiol

Synthesis of Nucleoside Triphosphates that Contain an Aminooxy Function for “Post-Amplification Labelling”

Preparation of the uridine and adenosine triphosphates 1 and 2 bearing a linker with a terminal aminooxy group is described. Both 1 and 2 react readily with the aldehydic fluorescein derivative 15. They could each be incorporated into a 330-mer fragment with T7 RNA polymerase.

The oxyamino-aldehyde coupling reaction: An efficient method for the derivatization of oligonucleotides

Abstract Oligonucleotides containing an aldehydic function at any preselected position in the sequence have been prepared using post-synthetic oxidation of an alkene as the key reaction. These were efficiently coupled to a reporter molecule tethered to an oxyamino linker.

Synthesis of Methylketone Containing Nucleoside Triphosphates for RNA Labelling

Abstract The three nucleoside triphosphates 1, 2 and 3 bearing a linker with a terminal methylketone group were prepared for incorporation into RNA fragments and post-labelling by the fluorescein derivative 4 containing an aminooxy group.

Fluorescent Labelling of Oligodeoxyribonucleotides by the Oxyamino-Aldehyde Coupling Reaction

Abstract We describe the reaction of oligonucleotides containing an aldehydic group at the 5′-end or inside the sequence with an oxyamino label. The reaction was found to be highly selective and represents an efficient method for derivatization of oligonucleotides.

Aryldiazomethane derivatives as reagents for site specific labeling of nucleic acids at phosphate

Abstract An efficient and direct labeling method based on direct alkylation of nucleic acids at phosphates by aryldiazomethane derivatives is described.

Labeling During Cleavage of Nucleic Acids for Their Detection on DNA Chips

Abstract A new and efficient strategy for labeling of nucleic acids prior to their hybridization on high density DNA chip has been developed. Our approach which combines the fragmentation and the labeling is based on the reactivity of the terminal phosphates of cleaved DNA and RNA fragments with a reporter molecule bearing aryldiazomethane group.

Synthesis and Biological Evaluation of Nucleoside Triphosphates Incorporating an Oxyamino Function for “Post-amplification Labelling”

Abstract We report a novel strategy for Post-Amplification Labelling based upon coupling nucleotide incorporating an oxyamino function with a fluorophore bearing an aldehydic group.