Ali Laayoun

Synthesis of Methylketone Containing Nucleoside Triphosphates for RNA Labelling

Abstract The three nucleoside triphosphates 1, 2 and 3 bearing a linker with a terminal methylketone group were prepared for incorporation into RNA fragments and post-labelling by the fluorescein derivative 4 containing an aminooxy group.

Universal labeling chemistry for nucleic acid detection on DNA-arrays.

Abstract We show here a new and efficient aqueous chemistry for labeling of any class of nucleic acids for their detection on DNA chip. The labels contain a diazo function as reactive moiety and biotin as detectable unit. The highly selective reaction of diazo group on the phosphate does not disrupt base pairing recognition and hybridization specificity.

Labeling during cleavage (LDC), a new labeling approach for RNA.

A new and efficient strategy for labeling of RNA sequences prior to their hybridization on high density DNA chip has been developed. Our approach which combines the fragmentation and the labeling is based on the reactivity of the 3′-phosphate of cleaved RNA fragments with a fluorescent molecule bearing aromatic bromomethyl function.

Aryldiazomethane derivatives as reagents for site specific labeling of nucleic acids at phosphate

Abstract An efficient and direct labeling method based on direct alkylation of nucleic acids at phosphates by aryldiazomethane derivatives is described.

Clip-Phen Conjugates for the Specific Cleavage of Nucleic Acids

For the first time Clip-Phen ( 1 ) was conjugated to oligonucleotides to provide very efficient tools for the cleavage of nucleic acids at specific positions. The synthesis of the conjugates as well as the cleavage experiments are reported.

Labeling During Cleavage of Nucleic Acids for Their Detection on DNA Chips

Abstract A new and efficient strategy for labeling of nucleic acids prior to their hybridization on high density DNA chip has been developed. Our approach which combines the fragmentation and the labeling is based on the reactivity of the terminal phosphates of cleaved DNA and RNA fragments with a reporter molecule bearing aryldiazomethane group.

New substituted aryldiazomethyl labels for high density DNA Chipbased nucleic acid testing

DNA and RNA labeling and detection are key steps in nucleic acid testing, particularly for molecular diagnostics applications. Here, we report a new class of aryldiazomethyl labels that include in their structure a biotin moiety as a detectable unit and a nitro substituted phenyl diazomethyl as a reactive group. The greatest reactivity towards phosphates of nucleic acids, the water solubility and the stability of these new molecules were demonstrated. These very important properties, which are the main requirements for nucleic acid labeling in aqueous conditions using automated protocols within integrated diagnostic devices, make them the perfect labeling tools for hybridization-based analysis, especially for high-density DNA chips.

Universal Labeling Chemistry for Nucleic Acid Detection on DNA Chips

An efficient strategy for nucleic acid labeling and analysis on deoxyribonucleic acid (DNA) chips has been developed. This approach, which combines the fragmentation and the labeling steps, is based on the reactivity of the phosphates of DNA and ribonucleic acid (RNA) fragments and is using reporter molecules bearing a bromomethyl- or aryldiazomethane-reactive group. In this chapter, we describe the preparation of the reactive label and protocols for efficient labeling of any nucleic acid sequence, DNA or RNA, prior to their hybridization, detection, and analysis on DNA chips.

Synthesis and Biological Evaluation of Nucleoside Triphosphates Incorporating an Oxyamino Function for “Post-amplification Labelling”

Abstract We report a novel strategy for Post-Amplification Labelling based upon coupling nucleotide incorporating an oxyamino function with a fluorophore bearing an aldehydic group.