Enjun Cheng

A pH‐Triggered, Fast‐Responding DNA Hydrogel

A fast, pH-responsive DNA hydrogel (see picture; right) was prepared by a three-armed DNA nanostructure (left) assembling together through the formation of intermolecular i-motif structures (middle). The hydrogel can be switched to the non-gel state in minutes by simply using environmental pH changes.

Self-assembled DNA hydrogels with designable thermal and enzymatic responsiveness.

or used as a programmable template to direct the assembly of nanoparticles. [ 14–17 ] Recently, the concept of DNA assembly has been expanded to construct “DNA hydrogels”, which are crosslinked networks swollen in an aqueous phase. [ 18–31 ] Though hydrogels have great potential in biological and medical applications, [ 32–36 ] such as drug and gene delivery, biosensing, and tissue engineering, studying the preparation of DNA hydrogels with designable properties is still in its early stages. In the past, several methods have been reported to prepare DNA hydrogels, for example, DNA directly extracted from the nucleus in nature, behaves like a long linear polymer and forms a hydrogel via physical entanglement or by chemical crosslinking of small molecules. [ 18 − 20] Similarly, DNA can be used as a negatively charged polymer and form a complex with cationic (poly)electrolytes through electrostatic interactions. [ 21 , 22 ] However, both methods treated DNA as a polymer and did not take advantage of the self-assembly of DNA into ordered structures, therefore, the resulting hydrogels lacked precise structural control and specifi c responses. Instead of using physical interactions, DNA can be covalently grafted onto synthetic polymers and serve as a cross-linker, the recognition of complementary DNA strands leads to crosslinking of polymer chains and causes hydrogel formation. [ 23 − 28] In general, the preparation of a DNA-polymer hybrid requires laborious modifi cation steps, and an easy and fast strategy to build tailored DNA hydrogels is desired. Luo and his coworkers have developed a new approach to construct

Improving the yield of mono-DNA-functionalized gold nanoparticles through dual steric hindrance.

A novel strategy of dual steric hindrance, which was obtained by Janus modification of gold nanoparticles (Au NPs) and volume exclusion of DNA, was adopted to prepare mono-DNA-modified Au NPs. The yield of mono-DNA-functionalized Au NPs significantly improved from 44 to 70% in the reaction between Au NPs and thiolated DNA. Furthermore, the specificity of mono-DNA-functionalized Au NPs was enhanced from 57 to 95%. The as-prepared Au NPs without postsynthetic treatment showed good controllability in self-assembly fabrication of complex nanostructures.

DNA pillars constructed from an i-motif stem and duplex branches.

At an acidic pH, cytosine-rich DNA strands can form i-motif tetramers. Pillar-like DNA structures are self-assembled with such i-motifs as the central stems. The central stem has some overhanging structures that can enable hybridization with complementary units by Watson-Crick pairing and, thus, multiple i-motifs can join to form the pillar.

DNA-templated CMV viral capsid proteins assemble into nanotubes

This communication describes the in vitro assembly of genetically recombinant Cucumber Mosaic Virus (CMV) viral capsid proteins (CPs) into biological nanotubes, several micrometres long yet with a diameter of only approximately 17 nm, triggered by double-stranded DNAs of different lengths.