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Featured researches published by Eon Seon Jin.


Biotechnology and Bioprocess Engineering | 2003

Microalgal biotechnology: Carotenoid production by the green algaeDunaliella salina

Eon Seon Jin; Anastasios Melis

Unicellular green algae of the genusDunaliella thrive in extreme environmental conditions such as high salinity, low pH, high irradiance and subzero temperatures. Species ofDunaliella are well known in the alga biotechnological industry and are employed widely for the production of valuable biochemicals, such as carotenoids. Some strains ofDunaliella are cultivated commercially in large outdoor ponds and are harvested to produce dry algal meals, such as polyunsaturated fatty acids and oils for the health food industry, and coloring agents for the food and cosmetic industries. During the past decade, the advances in molecular biology and biochemistry of microalgae, along with the advances in biotechnology of microalgal mass cultivation, enabled this microalga to become a staple of commercial exploitation. In particular, the advent of molecular biology and mutagenesis inDunaliella has permitted enhancements in the carotenoids content of this green alga, making it more attractive for biotechnological applications. Accordingly, the present review summarizes the recent developments and advances in biotechnology of carotenoid production inDunaliella.


Biotechnology and Bioprocess Engineering | 2013

Combination of 1,4-naphthoquinone with benzothiazoles had selective algicidal effects against harmful algae

Hoi Lym Kwon; Jae-Ho Kim; Dong Hee Na; Dae Heung Byeun; Ying Wu; Si Wouk Kim; Eon Seon Jin; Hoon Cho

A series of naphthoquinone-benzothiazole conjugates were synthesized as algicides, and their efficacies against harmful algal blooming species, such as Chattonella marina, Heterosigma akashiwo and Cochlodinium polykrikoides, were examined. The introduction of substituted benzothiazole at the C2 position of 1,4-naphthoquinone (compounds 1–9) resulted in higher algicidal activity against C. polykrikoides than the C6 conjugates (compounds 10–20). On the other hand, of the C6 conjugates, compounds 11 and 12 exhibited better algicidal activity against H. akashiwo, C. marina, and C. polykrikoides than the C2 conjugates. Further structure-activity analysis indicated that a replacement of the methoxy groups with hydroxyl groups (compounds 21–26) decreased the algicidal activity significantly. Among the various synthetic naphthoquinonebezothiazole conjugates tested, compound 12 was found to affect the most significant decrease in the level of C. polykrikoides growth, with an IC50 of 0.19 μM. Compound 11 was found to be the most potent inhibitor against H. akashiwo and C. polykrikoides, with IC50 values of 0.32 and 0.12 μM, respectively. Overall, these results highlight a possible method for controlling and inhibiting red tide forming algae using NQ derivatives.


Bioresource Technology | 2018

Improvement in modular scalability of polymeric thin-film photobioreactor for autotrophic culturing of Haematococcus pluvialis using industrial flue gas

Yoon Young Choi; Min Eui Hong; Eon Seon Jin; Han Min Woo; Sang Jun Sim

The aim of this study is investigate the effect of column diameter (D), height/diameter (H/D) ratio, and gas flow rate on microalgae cultivation, Haematococcus pluvialis. Bubble column reactors with various D and H/D ratio were tested to assess the hydrodynamic properties and biomass production performance. Then, H. pluvialis was cultured under outdoor autotrophic conditions using industrial flue gas. By optimizing the reactor module, reactor volume increased to 354% with minimized biomass loss. Compared to the control, developed module showed biomass and astaxanthin productivity of 0.052 versus 0.053 g/L/day, and 1.48 versus 1.47 mg/L/day, respectively. Consequently, biomass productivity was maintained with increased reactor scale, and the result is applicable to the scale up of overall microalgae cultivation process.


Biotechnology and Bioengineering | 2018

Photoautotrophic production of macular pigment in a Chlamydomonas reinhardtii strain generated by using DNA-free CRISPR-Cas9 RNP-mediated mutagenesis

Kwangryul Baek; Jihyeon Yu; Jooyeon Jeong; Sang Jun Sim; Sangsu Bae; Eon Seon Jin

Lutein and zeaxanthin are dietary carotenoids reported to be protective against age‐related macular degeneration. Recently, the green alga Chlamydomonas reinhardtii has received attention as a photosynthetic cell factory, but the potential of this alga for carotenoid production has not yet been evaluated. In this study, we selected the C. reinhardtii CC‐4349 strain as the best candidate among seven laboratory strains tested for carotenoid production. A knock‐out mutant of the zeaxanthin epoxidase gene induced by preassembled DNA‐free CRISPR‐Cas9 ribonucleoproteins in the CC‐4349 strain had a significantly higher zeaxanthin content (56‐fold) and productivity (47‐fold) than the wild type without the reduction in lutein level. Furthermore, we produced eggs fortified with lutein (2‐fold) and zeaxanthin (2.2‐fold) by feeding hens a diet containing the mutant. Our results clearly demonstrate the possibility of cost‐effective commercial use of microalgal mutants induced by DNA‐free CRISPR‐Cas9 ribonucleoproteins in algal biotechnology for the production of high‐value products.


FEBS Letters | 2016

NMR study of the antifreeze activities of active and inactive isoforms of a type III antifreeze protein

Seo-Ree Choi; Yeo-Jin Seo; Minjae Kim; Yumi Eo; Hee-Chul Ahn; Ae-Ree Lee; Chin-Ju Park; Kyoung-Seok Ryu; Hae-Kap Cheong; Shim Sung Lee; Eon Seon Jin; Joon-Hwa Lee

The quaternary‐amino‐ethyl 1 (QAE1) isoforms of type III antifreeze proteins (AFPs) prevent the growth of ice crystals within organisms living in polar regions. We determined the antifreeze activity of wild‐type and mutant constructs of the Japanese notched‐fin eelpout (Zoarces elongates Kner) AFP8 (nfeAFP8) and characterized the structural and dynamics properties of their ice‐binding surface using NMR. We found that the three constructs containing the V20G mutation were incapable of stopping the growth of ice crystals and exhibited structural changes, as well as increased conformational flexibility, in the first 310 helix (residues 18–22) of the sequence. Our results suggest that the inactive nfeAFP8s are incapable of anchoring water molecules due to the unusual and flexible backbone conformation of their primary prism plane‐binding surface.


Biosensors and Bioelectronics | 2019

A new coccolith modified electrode-based biosensor using a cognate pair of aptamers with sandwich-type binding

Sang Hoon Kim; Onyou Nam; Eon Seon Jin; Man Bock Gu

In this study, we report a cognate pair of the aptamer-based sandwich-type electrochemical biosensor for type 2 diabetes biomarker (Vaspin) using coccolith modified electrodeposited on the screen-printed gold electrode (CME-SPGE). The coccolith derived from E. huxleyi used in this study were known to be highly-structured microparticles with many nano-sized pores. The CME-SPGE was successfully fabricated by drop-casting coccoliths, followed by Au sputtering and electrodeposition of Au. On this CME-SPGE electrode, the sandwich-type electrochemical aptasensor was fabricated by using a cognate pair of aptamers. The morphological, electrochemical characteristics and the performances of both the CME-SPGE and the completely fabricated sandwich-type aptasensor were investigated by SEM, EDAX, cyclic voltammetry, and chronoamperometry. Due to the synergic effect of a cognate pair of aptamers on CME-SPGE, this newly developed sandwich-type electrochemical biosensor for Vaspin showed high specificity, and good sensitivity with a limit of detection (LOD) of 298 pM, along with more widen the linear range. To the best of our knowledge, this is the first report about the use of a coccolith modified electrode with a cognate pair aptamer resulting in sandwich-type binding in an electrochemical biosensor. With the advantages of using highly-structured biomineral microparticles and a cognate pair of aptamers, this new study may pave the innovative way to design a novel sandwich-type electrochemical aptasensor platform.


Applied Biochemistry and Biotechnology | 2012

Expression and Characterization of Codon-Optimized Carbonic Anhydrase from Dunaliella Species for CO2 Sequestration Application

Bashistha Kumar Kanth; Kiha Min; Shipra Kumari; Hancheol Jeon; Eon Seon Jin; Jinwon Lee; Seung Pil Pack


Algal Research-Biomass Biofuels and Bioproducts | 2016

Synergistic effect of multiple stress conditions for improving microalgal lipid production

Ho Seok Kwak; Jaoon Young Hwan Kim; Han Min Woo; Eon Seon Jin; Byoung Koun Min; Sang Jun Sim


Archive | 2013

광 유도성 프로모터 및 이를 포함하는 유전자 발현 시스템

Eon Seon Jin; 진언선; Jae-Hyeok Lee; 이재혁; Seung Hye Park; 박승혜; Kwang Ryul Baek; 백광열


Archive | 2013

LIGHT-INDUCIBLE PROMOTER AND GENE EXPRESSION SYSTEM CONTAINING SAME

Eon Seon Jin; Jae Hyeok Lee; Seung Hye Park; Kwang Ryul Baek

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Han Min Woo

Sungkyunkwan University

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Ae-Ree Lee

Gyeongsang National University

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Byoung Koun Min

Korea Institute of Science and Technology

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Dong Hee Na

Kyungpook National University

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